Display options
Cite
Jupa R, Didi V, Hejátko J, et al. An improved method for the visualization of conductive vessels in Arabidopsis thaliana inflorescence stems. Front Plant Sci. 2015;6:211doi: 10.3389/fpls.2015.00211.
Jupa, R., Didi, V., Hejátko, J., & Gloser, V. (2015). An improved method for the visualization of conductive vessels in Arabidopsis thaliana inflorescence stems. Frontiers in plant science, 6211. https://doi.org/10.3389/fpls.2015.00211
Jupa, Radek, et al. "An improved method for the visualization of conductive vessels in Arabidopsis thaliana inflorescence stems." Frontiers in plant science vol. 6 (2015): 211. doi: https://doi.org/10.3389/fpls.2015.00211
Jupa R, Didi V, Hejátko J, Gloser V. An improved method for the visualization of conductive vessels in Arabidopsis thaliana inflorescence stems. Front Plant Sci. 2015 Apr 09;6:211. doi: 10.3389/fpls.2015.00211. eCollection 2015. PMID: 25914701; PMCID: PMC4391271.
Copy Download .nbib Format: NLM
Share it on

Front Plant Sci. 2015 Apr 09;6:211. doi: 10.3389/fpls.2015.00211. eCollection 2015.

An improved method for the visualization of conductive vessels in Arabidopsis thaliana inflorescence stems.

Frontiers in plant science

Radek Jupa, Vojtěch Didi, Jan Hejátko, Vít Gloser

Affiliations

  1. Department of Experimental Biology, Faculty of Science, Masaryk University Brno, Czech Republic.
  2. Functional Genomics and Proteomics of Plants, Central European Institute of Technology, Masaryk University Brno, Czech Republic.

PMID: 25914701 PMCID: PMC4391271 DOI: 10.3389/fpls.2015.00211

Abstract

Dye perfusion is commonly used for the identification of conductive elements important for the study of xylem development as well as precise hydraulic estimations. The tiny size of inflorescence stems, the small amount of vessels in close arrangement, and high hydraulic resistivity delimit the use of the method for quantification of the water conductivity of Arabidopsis thaliana, one of the recently most extensively used plant models. Here, we present an extensive adjustment to the method in order to reliably identify individual functional (conductive) vessels. Segments of inflorescence stems were sealed in silicone tubes to prevent damage and perfused with a dye solution. Our results showed that dyes often used for staining functional xylem elements (safranin, fuchsine, toluidine blue) failed with Arabidopsis. In contrast, Fluorescent Brightener 28 dye solution perfused through segments stained secondary cell walls of functional vessels, which were clearly distinguishable in native cross sections. When compared to identification based on the degree of development of secondary cell walls, identification with the help of dye perfusion revealed a significantly lower number of functional vessels and values of theoretical hydraulic conductivity. We found that lignified but not yet functional vessels form a substantial portion of the xylem in apical and basal segments of Arabidopsis and, thus, significantly affect the analyzed functional parameters of xylem. The presented methodology enables reliable identification of individual functional vessels, allowing thus estimations of hydraulic conductivities to be improved, size distributions and vessel diameters to be refined, and data variability generally to be reduced.

Keywords: Fluorescent Brightener 28; conductive elements; dye perfusion; fluorescence; hydraulic conductivity; vessel; xylem

References

  1. J Appl Bacteriol. 1995 Mar;78(3):309-15 - PubMed
  2. Tree Physiol. 2006 Jun;26(6):689-701 - PubMed
  3. Plant Cell. 2009 Jul;21(7):2008-21 - PubMed
  4. Plant Physiol. 2004 Jun;135(2):653-9 - PubMed
  5. Nature. 2012 Nov 29;491(7426):752-5 - PubMed
  6. J Microsc. 2008 Dec;232(3):618-22 - PubMed
  7. Plant Cell Environ. 2010 Jul;33(7):1059-69 - PubMed
  8. Plant Cell Environ. 2011 Apr;34(4):655-68 - PubMed
  9. J Exp Bot. 2012 Feb;63(3):1081-94 - PubMed
  10. Am J Bot. 2006 Nov;93(11):1567-76 - PubMed
  11. J Clin Microbiol. 2002 Jun;40(6):1892-901 - PubMed
  12. Plant Cell Environ. 2010 Mar;33(3):431-43 - PubMed
  13. Tree Physiol. 2007 Jul;27(7):993-9 - PubMed
  14. J Exp Bot. 2002 Mar;53(368):489-503 - PubMed
  15. Am J Bot. 2013 Feb;100(2):322-31 - PubMed
  16. Physiol Plant. 2002 Apr;114(4):594-600 - PubMed
  17. Tree Physiol. 2005 Mar;25(3):269-75 - PubMed
  18. J Exp Bot. 2013 May;64(8):2295-305 - PubMed
  19. Curr Opin Biotechnol. 2011 Apr;22(2):293-9 - PubMed
  20. Nature. 2008 Sep 11;455(7210):208-12 - PubMed
  21. J Plant Res. 2013 Mar;126(2):223-31 - PubMed
  22. Planta. 2013 Aug;238(2):307-15 - PubMed

Publication Types