Display options
Cite
Shin Y, Hyeon J, Lee B, et al. PHH3 as an Ancillary Mitotic Marker in Gastrointestinal Stromal Tumors. J Pathol Transl Med. 2015;49(1):23-9doi: 10.4132/jptm.2014.10.08.
Shin, Y., Hyeon, J., Lee, B., Ha, S. Y., Hong, M. E., Do, I. G., & Kim, K. M. (2015). PHH3 as an Ancillary Mitotic Marker in Gastrointestinal Stromal Tumors. Journal of pathology and translational medicine, 49(1), 23-9. https://doi.org/10.4132/jptm.2014.10.08
Shin, Yooju, et al. "PHH3 as an Ancillary Mitotic Marker in Gastrointestinal Stromal Tumors." Journal of pathology and translational medicine vol. 49,1 (2015): 23-9. doi: https://doi.org/10.4132/jptm.2014.10.08
Shin Y, Hyeon J, Lee B, Ha SY, Hong ME, Do IG, Kim KM. PHH3 as an Ancillary Mitotic Marker in Gastrointestinal Stromal Tumors. J Pathol Transl Med. 2015 Jan;49(1):23-9. doi: 10.4132/jptm.2014.10.08. Epub 2015 Jan 15. PMID: 25812654; PMCID: PMC4357403.
Copy Download .nbib Format: NLM
Share it on

J Pathol Transl Med. 2015 Jan;49(1):23-9. doi: 10.4132/jptm.2014.10.08. Epub 2015 Jan 15.

PHH3 as an Ancillary Mitotic Marker in Gastrointestinal Stromal Tumors.

Journal of pathology and translational medicine

Yooju Shin, Jiyeon Hyeon, Boram Lee, Sang Yun Ha, Min Eui Hong, In Gu Do, Kyoung-Mee Kim

Affiliations

  1. Department of Pathology and Translational Genomics, Samsung Medical Center, Sungkyunkwan University College of Medicine, Seoul, Korea.

PMID: 25812654 PMCID: PMC4357403 DOI: 10.4132/jptm.2014.10.08

Abstract

BACKGROUND: Counting mitoses is subjective and time-consuming. The adjunctive diagnostic utility of a recently reported mitotic marker, phosphohistone H3 (PHH3), was investigated in gastrointestinal stromal tumors (GISTs).

METHODS: We reviewed 77 GISTs for several proliferative indices. These included the mitotic count per 50 high power fields (HPFs), the immunohistochemical Ki- 67 labeling index and the immunohistochemical PHH3 mitotic index (MI). For comparison, Spearman's rank correlation and interclass correlation coefficient were used.

RESULTS: Mitotic counts ranged from 0-138 (mean, 7.57±2.34) and the PHH3 MI ranged from 0-126 per 50 HPFs (mean, 9.61±2.27). We found a positive correlation between mitotic counts and PHH3 MI (r=0.810, p<.001). The inter-observer correlation coefficient for three participants was 0.975 for mitotic counts and 0.940 for the PHH3 MI. When using the PHH3 MI instead of mitotic counts in the Armed Forces Institute of Pathology (AFIP) stratification criteria, 10 cases were reclassified. In one patient with a mitotic count of 2 and a PHH3 MI of 6 per 50 HPFs, distant metastasis occurred.

CONCLUSIONS: In GISTs, the PHH3 MI correlated adequately with mitotic counts and can be used as a useful adjunctive to count mitotic figures efficiently.

Keywords: Biological marker; Gastrointestinal stromal tumors; Ki-67; Mitosis

References

  1. Cytometry. 1998 Jun 1;32(2):71-7 - PubMed
  2. Am J Clin Pathol. 2007 Jul;128(1):118-25 - PubMed
  3. Am J Surg Pathol. 2006 Apr;30(4):477-89 - PubMed
  4. Int J Gynecol Pathol. 2009 Jul;28(4):316-21 - PubMed
  5. Am J Surg Pathol. 2006 Jan;30(1):83-9 - PubMed
  6. Am J Surg Pathol. 2005 Jan;29(1):52-68 - PubMed
  7. J Clin Oncol. 2004 Sep 15;22(18):3813-25 - PubMed
  8. Hum Pathol. 2002 May;33(5):459-65 - PubMed
  9. Dig Dis Sci. 2002 Oct;47(10):2247-53 - PubMed
  10. Hum Reprod. 2003 Jun;18(6):1185-93 - PubMed
  11. Appl Immunohistochem Mol Morphol. 2006 Sep;14(3):296-302 - PubMed
  12. Hepatogastroenterology. 2007 Mar;54(74):438-41 - PubMed
  13. Arch Pathol Lab Med. 2006 Oct;130(10):1466-78 - PubMed
  14. Am J Clin Pathol. 1995 Jan;103(1):41-7 - PubMed
  15. Am J Clin Pathol. 2011 Aug;136(2):252-9 - PubMed
  16. J Immunol. 1984 Oct;133(4):1710-5 - PubMed
  17. Brain Tumor Pathol. 2009;26(2):51-7 - PubMed
  18. Hum Pathol. 1997 Feb;28(2):160-5 - PubMed
  19. Chromosoma. 1997 Nov;106(6):348-60 - PubMed
  20. J Clin Pathol. 2006 Jul;59(7):706-10 - PubMed
  21. Am J Dermatopathol. 2008 Apr;30(2):117-22 - PubMed
  22. Anticancer Res. 2013 Feb;33(2):461-7 - PubMed
  23. Am J Dermatopathol. 2012 May;34(3):266-9 - PubMed
  24. Am J Surg Pathol. 2004 Nov;28(11):1532-6 - PubMed
  25. Int J Clin Exp Pathol. 2010 May 05;3(5):461-71 - PubMed

Publication Types