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Chaand M, Miller KA, Sofia MK, et al. Type 3 Secretion System Island Encoded Proteins Required for Colonization by Non-O1/non-O139 Serogroup . Infect Immun. 2015;83(7):2862-2869doi: 10.1128/IAI.03020-14.
Chaand, M., Miller, K. A., Sofia, M. K., Schlesener, C., Weaver, J. W., Sood, V., & Dziejman, M. (2015). Type 3 Secretion System Island Encoded Proteins Required for Colonization by Non-O1/non-O139 Serogroup . Infection and immunity, 83(7), 2862-2869. https://doi.org/10.1128/IAI.03020-14
Chaand, Mudit, et al. "Type 3 Secretion System Island Encoded Proteins Required for Colonization by Non-O1/non-O139 Serogroup ." Infection and immunity vol. 83,7 (2015): 2862-2869. doi: https://doi.org/10.1128/IAI.03020-14
Chaand M, Miller KA, Sofia MK, Schlesener C, Weaver JW, Sood V, Dziejman M. Type 3 Secretion System Island Encoded Proteins Required for Colonization by Non-O1/non-O139 Serogroup . Infect Immun. 2015 Jul;83(7):2862-2869. doi: 10.1128/IAI.03020-14. Epub 2015 May 04. PMID: 25939511; PMCID: PMC4468554.
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Infect Immun. 2015 Jul;83(7):2862-2869. doi: 10.1128/IAI.03020-14. Epub 2015 May 04.

Type 3 Secretion System Island Encoded Proteins Required for Colonization by Non-O1/non-O139 Serogroup .

Infection and immunity

Mudit Chaand, Kelly A Miller, Madeline K Sofia, Cory Schlesener, Jacob W A Weaver, Vibha Sood, Michelle Dziejman

Affiliations

  1. Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, Rochester, NY.
  2. Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, Rochester, NY [email protected].

PMID: 25939511 PMCID: PMC4468554 DOI: 10.1128/IAI.03020-14

Abstract

Vibrio cholerae is a genetically diverse species, and pathogenic strains can encode different virulence factors that mediate colonization and secretory diarrhea. Although the toxin co-regulated pilus (TCP) is the primary colonization factor in epidemic causing V. cholerae strains, other strains do not encode TCP and instead promote colonization via the activity of a type three secretion system (T3SS). Using the infant mouse model and T3SS-positive O39 serogroup strain AM-19226, we sought to determine which of 12 previously identified, T3SS translocated proteins (Vops) are important for host colonization. We constructed in frame deletions in each of the 12 loci in strain AM-19226, and identified five Vop deletion strains, including ΔVopM, which were severely attenuated for colonization. Interestingly, a subset of deletion strains was also incompetent for effector protein transport. Our collective data therefore suggest that several translocated proteins may also function as components of the structural apparatus or translocation machinery, and indicate that while VopM is critical for establishing an infection, the combined activities of other effectors may also contribute to the ability of T3SS-positive strains to colonize host epithelial cell surfaces.

Copyright © 2015, American Society for Microbiology. All Rights Reserved.

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