Display options
Cite
Zhand S, Karami C, Hosseinzadeh Adli A, et al. Correlation Between Hepatitis B G1896A Precore Mutations and HBeAg in Chronic HBV Patients. Jundishapur J Microbiol. 2015;8(2):e17126doi: 10.5812/jjm.17126.
Zhand, S., Karami, C., Hosseinzadeh Adli, A., Tabarraei, A., Khodabakhshi, B., & Moradi, A. (2015). Correlation Between Hepatitis B G1896A Precore Mutations and HBeAg in Chronic HBV Patients. Jundishapur journal of microbiology, 8(2), e17126. https://doi.org/10.5812/jjm.17126
Zhand, Sareh, et al. "Correlation Between Hepatitis B G1896A Precore Mutations and HBeAg in Chronic HBV Patients." Jundishapur journal of microbiology vol. 8,2 (2015): e17126. doi: https://doi.org/10.5812/jjm.17126
Zhand S, Karami C, Hosseinzadeh Adli A, Tabarraei A, Khodabakhshi B, Moradi A. Correlation Between Hepatitis B G1896A Precore Mutations and HBeAg in Chronic HBV Patients. Jundishapur J Microbiol. 2015 Feb 01;8(2):e17126. doi: 10.5812/jjm.17126. eCollection 2015 Feb. PMID: 25825644; PMCID: PMC4376977.
Copy Download .nbib Format: NLM
Share it on

Jundishapur J Microbiol. 2015 Feb 01;8(2):e17126. doi: 10.5812/jjm.17126. eCollection 2015 Feb.

Correlation Between Hepatitis B G1896A Precore Mutations and HBeAg in Chronic HBV Patients.

Jundishapur journal of microbiology

Sareh Zhand, Chiman Karami, Ahmad Hosseinzadeh Adli, Alijan Tabarraei, Behnaz Khodabakhshi, Abdolvahab Moradi

Affiliations

  1. Department of Microbiology, School of Medicine, Golestan University of Medical Sciences, Gorgan, IR Iran.
  2. Infectious Diseases Research Centre, Golestan University of Medical Sciences, Gorgan, IR Iran.

PMID: 25825644 PMCID: PMC4376977 DOI: 10.5812/jjm.17126

Abstract

BACKGROUND: Hepatitis B virus (HBV) infection is an important health concern worldwide, with critical outcomes. Hepatitis B e antigen (HBeAg) negative chronic hepatitis B is frequently caused by a mutation (G1896A) in the hepatitis B virus (HBV) precore (PC) reading frame, which creates a stop codon, causing premature termination of the HBe protein.

OBJECTIVES: This study aimed to investigate the G1896A PC mutation and its effect on HBeAg detection in chronic HBV patients.

PATIENTS AND METHODS: In this study, 120 chronic HBV patients neither vaccinated or who had benefited from immunoglobulin therapy, were recruited. The HBV-DNA was extracted from plasma and polymerase chain reaction (PCR) was performed. Positive PCR products were subjected to automated sequencing. The HBV serological markers [hepatitis B s antigen (HBsAg), HBeAg] were tested.

RESULTS: One hundred out of 120 (83.3%) patients were HBeAg negative and 100% were HBsAg positive. The comparison of nucleotide sequences with the reference sequence (Accession number: AB033559) in HBeAg negative patients showed that there was a high rate of mutations in G1896A (93.18%).

CONCLUSIONS: This study indicates that the rate of G1896A mutation at the PC region among HBeAg negative patients, in the Golestan province of Iran, was similar to the average rate encountered in other parts of Iran. The PC stop codon mutation was detected in 93.18% of HBeAg negative patients. Further studies with larger sample sizes are required to elucidate the exact role of these mutations in the clinical course of chronic HBV infection.

Keywords: Hepatitis B e Antigens; Hepatitis B, Chronic; Iran; Mutation

References

  1. Indian J Med Sci. 2007 May;61(5):263-8 - PubMed
  2. Korean J Intern Med. 1997 Jun;12(2):201-7 - PubMed
  3. Hepatology. 1983 Sep-Oct;3(5):656-62 - PubMed
  4. Hepatology. 2001 Oct;34(4 Pt 1):617-24 - PubMed
  5. J Med Virol. 1999 Apr;57(4):337-44 - PubMed
  6. Infection. 1999 Nov-Dec;27(6):357-60 - PubMed
  7. J Infect. 2007 Mar;54(3):291-7 - PubMed
  8. J Med Virol. 1999 Jul;58(3):193-5 - PubMed
  9. Gastroenterology. 1993 Sep;105(3):845-50 - PubMed
  10. BMC Microbiol. 2001;1:10 - PubMed
  11. J Clin Virol. 2005 Jan;32(1):53-9 - PubMed
  12. BMC Gastroenterol. 2006 Jul 24;6:20 - PubMed
  13. J Virol. 2006 Jan;80(2):587-95 - PubMed
  14. J Hepatol. 2004 Mar;40(3):507-14 - PubMed
  15. Infection. 2004 Feb;32(1):24-9 - PubMed
  16. J Med Virol. 2003 Mar;69(3):324-30 - PubMed
  17. Hepatology. 1999 Mar;29(3):976-84 - PubMed
  18. J Virol. 1996 Sep;70(9):5845-51 - PubMed
  19. J Gen Virol. 1988 Oct;69 ( Pt 10):2575-83 - PubMed
  20. Virus Genes. 2012 Jun;44(3):382-7 - PubMed
  21. J Med Virol. 2000 Feb;60(2):107-12 - PubMed
  22. J Viral Hepat. 2002 Jan;9(1):52-61 - PubMed
  23. Microbes Infect. 2000 May;2(6):607-12 - PubMed
  24. J Hepatol. 2000 Sep;33(3):430-9 - PubMed
  25. J Gen Virol. 2002 Jun;83(Pt 6):1267-80 - PubMed
  26. Hepatology. 2000 May;31(5):1037-44 - PubMed
  27. J Gastrointestin Liver Dis. 2007 Dec;16(4):403-6 - PubMed
  28. J Res Med Sci. 2009 Jul;14(4):249-58 - PubMed
  29. J Med Virol. 2003 Aug;70(4):529-36 - PubMed
  30. Gut. 1984 Nov;25(11):1283-7 - PubMed
  31. Epidemiol Infect. 2000 Aug;125(1):169-74 - PubMed

Publication Types