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Sattar S, Bennett NJ, Wen WX, et al. Ff-nano, short functionalized nanorods derived from Ff (f1, fd, or M13) filamentous bacteriophage. Front Microbiol. 2015;6:316doi: 10.3389/fmicb.2015.00316.
Sattar, S., Bennett, N. J., Wen, W. X., Guthrie, J. M., Blackwell, L. F., Conway, J. F., & Rakonjac, J. (2015). Ff-nano, short functionalized nanorods derived from Ff (f1, fd, or M13) filamentous bacteriophage. Frontiers in microbiology, 6316. https://doi.org/10.3389/fmicb.2015.00316
Sattar, Sadia, et al. "Ff-nano, short functionalized nanorods derived from Ff (f1, fd, or M13) filamentous bacteriophage." Frontiers in microbiology vol. 6 (2015): 316. doi: https://doi.org/10.3389/fmicb.2015.00316
Sattar S, Bennett NJ, Wen WX, Guthrie JM, Blackwell LF, Conway JF, Rakonjac J. Ff-nano, short functionalized nanorods derived from Ff (f1, fd, or M13) filamentous bacteriophage. Front Microbiol. 2015 Apr 20;6:316. doi: 10.3389/fmicb.2015.00316. eCollection 2015. PMID: 25941520; PMCID: PMC4403547.
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Front Microbiol. 2015 Apr 20;6:316. doi: 10.3389/fmicb.2015.00316. eCollection 2015.

Ff-nano, short functionalized nanorods derived from Ff (f1, fd, or M13) filamentous bacteriophage.

Frontiers in microbiology

Sadia Sattar, Nicholas J Bennett, Wesley X Wen, Jenness M Guthrie, Len F Blackwell, James F Conway, Jasna Rakonjac

Affiliations

  1. Institute of Fundamental Sciences, Massey University Palmerston North, New Zealand.
  2. Institute of Fundamental Sciences, Massey University Palmerston North, New Zealand ; Science Haven Limited, Palmerston North New Zealand.
  3. University of Pittsburgh School of Medicine Pittsburgh, PA, USA.

PMID: 25941520 PMCID: PMC4403547 DOI: 10.3389/fmicb.2015.00316

Abstract

F-specific filamentous phage of Escherichia coli (Ff: f1, M13, or fd) are long thin filaments (860 nm × 6 nm). They have been a major workhorse in display technologies and bionanotechnology; however, some applications are limited by the high length-to-diameter ratio of Ff. Furthermore, use of functionalized Ff outside of laboratory containment is in part hampered by the fact that they are genetically modified viruses. We have now developed a system for production and purification of very short functionalized Ff-phage-derived nanorods, named Ff-nano, that are only 50 nm in length. In contrast to standard Ff-derived vectors that replicate in E. coli and contain antibiotic-resistance genes, Ff-nano are protein-DNA complexes that cannot replicate on their own and do not contain any coding sequences. These nanorods show an increased resistance to heating at 70(∘)C in 1% SDS in comparison to the full-length Ff phage of the same coat composition. We demonstrate that functionalized Ff-nano particles are suitable for application as detection particles in sensitive and quantitative "dipstick" lateral flow diagnostic assay for human plasma fibronectin.

Keywords: M13 phage; f1 phage; fd phage; fibronectin-binding protein; filamentous phage; lateral flow; nanorod; phage display

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