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Farajzadah Sheikh A, Saki N, Roointan M, et al. Identification of Alloiococcus otitidis, Streptococcus pneumoniae, Moraxella catarrhalis and Haemophilus influenzae in Children With Otitis Media With Effusion. Jundishapur J Microbiol. 2015;8(3):e17985doi: 10.5812/jjm.17985.
Farajzadah Sheikh, A., Saki, N., Roointan, M., Ranjbar, R., Yadyad, M. J., Kaydani, A., Aslani, S., Babaei, M., & Goodarzi, H. (2015). Identification of Alloiococcus otitidis, Streptococcus pneumoniae, Moraxella catarrhalis and Haemophilus influenzae in Children With Otitis Media With Effusion. Jundishapur journal of microbiology, 8(3), e17985. https://doi.org/10.5812/jjm.17985
Farajzadah Sheikh, Ahmad, et al. "Identification of Alloiococcus otitidis, Streptococcus pneumoniae, Moraxella catarrhalis and Haemophilus influenzae in Children With Otitis Media With Effusion." Jundishapur journal of microbiology vol. 8,3 (2015): e17985. doi: https://doi.org/10.5812/jjm.17985
Farajzadah Sheikh A, Saki N, Roointan M, Ranjbar R, Yadyad MJ, Kaydani A, Aslani S, Babaei M, Goodarzi H. Identification of Alloiococcus otitidis, Streptococcus pneumoniae, Moraxella catarrhalis and Haemophilus influenzae in Children With Otitis Media With Effusion. Jundishapur J Microbiol. 2015 Mar 21;8(3):e17985. doi: 10.5812/jjm.17985. eCollection 2015 Mar. PMID: 25861433; PMCID: PMC4386075.
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Jundishapur J Microbiol. 2015 Mar 21;8(3):e17985. doi: 10.5812/jjm.17985. eCollection 2015 Mar.

Identification of Alloiococcus otitidis, Streptococcus pneumoniae, Moraxella catarrhalis and Haemophilus influenzae in Children With Otitis Media With Effusion.

Jundishapur journal of microbiology

Ahmad Farajzadah Sheikh, Nader Saki, Mitra Roointan, Reza Ranjbar, Mohammad Jaafar Yadyad, Abbas Kaydani, Sajad Aslani, Mansoor Babaei, Hamed Goodarzi

Affiliations

  1. Department of Microbiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran.
  2. Hearing and Speech Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran.
  3. Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, IR Iran.
  4. Infectious and Tropical Diseases Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran.
  5. Department of Virology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran.
  6. Health Management Research Center, Baqiyatallah University of Medical Sciences, Tehran, IR Iran.

PMID: 25861433 PMCID: PMC4386075 DOI: 10.5812/jjm.17985

Abstract

BACKGROUND: Based on many studies, otitis media with effusion (OME) is one of the major causes of childhood hearing loss, social malformation and medical costs. The pathogenesis still remains unclear, though it is known that this complication is closely related to bacterial infections. Alloiococcus otitidis, Haemophilus influenzae, Streptococcus pneumoniae and Moraxella catarrhalis are the most common bacterial pathogens isolated from middle ear effusions (MEEs).

OBJECTIVES: Due to the prevalence of OME in children, we decided to investigate bacterial agents that cause diseases such as A. otitidis, H. influenzae, S. pneumonia and M. catarrhalis in these subjects.

PATIENTS AND METHODS: Forty-five children between one and 15 years of age were selected for this study. Seventy specimens were collected from MEE by myringotomy and inoculated in PBS buffer. Conventional culture and PCR methods were used for identification of bacterial agents.

RESULTS: The bacterial cultures in 8.6% of samples were positive by conventional culture, with A. otitidis, M. catarrhalis and S. pneumoniae present in 1.4%, 2.9% and 4.3% of samples, respectively. No H. influenzae was isolated. By the PCR method, A. otitidis was the most frequently isolated bacterium, found in 25.7% of samples, followed by S. pneumoniae, M. catarrhalis and H. influenzae, which were identified in 20%, 12% and 20% of samples, respectively. Overall, 55 out of 70 samples were positive by both the PCR and culture method.

CONCLUSIONS: It can be concluded that A. otitidis was the major causative agent of MEE in children with OME. Therefore clinicians should be aware that bacterial infection plays an important role in the progression of acute otitis media to OME in children of our region.

Keywords: Haemophilus influenzae; Moraxella catarrhalis; Otitis Media with Effusion; PCR; Streptococcus pneumonia

References

  1. Microbes Infect. 2000 Apr;2(5):547-59 - PubMed
  2. J Clin Microbiol. 1995 Nov;33(11):2876-80 - PubMed
  3. J Clin Microbiol. 1997 Nov;35(11):2854-8 - PubMed
  4. Eur J Immunol. 2006 Jun;36(6):1527-36 - PubMed
  5. Auris Nasus Larynx. 2010 Jun;37(3):269-73 - PubMed
  6. J Pediatr (Rio J). 2004 Jan-Feb;80(1):41-8 - PubMed
  7. J Clin Microbiol. 2006 Mar;44(3):946-9 - PubMed
  8. FEMS Immunol Med Microbiol. 2007 Oct;51(1):163-70 - PubMed
  9. J Clin Microbiol. 1988 Dec;26(12):2544-8 - PubMed
  10. J Clin Microbiol. 1998 May;36(5):1185-8 - PubMed
  11. Acta Otorrinolaringol Esp. 2007 Nov;58(9):408-12 - PubMed
  12. J Korean Med Sci. 2004 Oct;19(5):735-8 - PubMed
  13. Auris Nasus Larynx. 2012 Aug;39(4):369-73 - PubMed
  14. Int J Pediatr Otorhinolaryngol. 2004 Jan;68(1):51-6 - PubMed
  15. Int J Pediatr Otorhinolaryngol. 2006 Jun;70(6):1009-14 - PubMed
  16. Clin Exp Otorhinolaryngol. 2012 Jun;5(2):81-5 - PubMed
  17. Otol Neurotol. 2008 Jun;29(4):526-30 - PubMed
  18. J Clin Microbiol. 2000 Jan;38(1):125-32 - PubMed

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