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Fatahinia M, Poormohamadi F, Zarei Mahmoudabadi A. Comparative Study of Esterase and Hemolytic Activities in Clinically Important Candida Species, Isolated From Oral Cavity of Diabetic and Non-diabetic Individuals. Jundishapur J Microbiol. 2015;8(3):e20893doi: 10.5812/jjm.20893.
Fatahinia, M., Poormohamadi, F., & Zarei Mahmoudabadi, A. (2015). Comparative Study of Esterase and Hemolytic Activities in Clinically Important Candida Species, Isolated From Oral Cavity of Diabetic and Non-diabetic Individuals. Jundishapur journal of microbiology, 8(3), e20893. https://doi.org/10.5812/jjm.20893
Fatahinia, Mahnaz, et al. "Comparative Study of Esterase and Hemolytic Activities in Clinically Important Candida Species, Isolated From Oral Cavity of Diabetic and Non-diabetic Individuals." Jundishapur journal of microbiology vol. 8,3 (2015): e20893. doi: https://doi.org/10.5812/jjm.20893
Fatahinia M, Poormohamadi F, Zarei Mahmoudabadi A. Comparative Study of Esterase and Hemolytic Activities in Clinically Important Candida Species, Isolated From Oral Cavity of Diabetic and Non-diabetic Individuals. Jundishapur J Microbiol. 2015 Mar 21;8(3):e20893. doi: 10.5812/jjm.20893. eCollection 2015 Mar. PMID: 25861439; PMCID: PMC4386073.
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Jundishapur J Microbiol. 2015 Mar 21;8(3):e20893. doi: 10.5812/jjm.20893. eCollection 2015 Mar.

Comparative Study of Esterase and Hemolytic Activities in Clinically Important Candida Species, Isolated From Oral Cavity of Diabetic and Non-diabetic Individuals.

Jundishapur journal of microbiology

Mahnaz Fatahinia, Farzad Poormohamadi, Ali Zarei Mahmoudabadi

Affiliations

  1. Department of Medical Mycology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran.
  2. Department of Medical Mycology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran ; Tropical and Infectious Disease Research Center, Department of Medical Mycology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran.

PMID: 25861439 PMCID: PMC4386073 DOI: 10.5812/jjm.20893

Abstract

BACKGROUND: Diabetes mellitus as a chronic metabolic disease occurs in patients with partial or complete deficiency of insulin secretion or disorder in action of insulin on tissue. The disease is known to provide conditions for overgrowth of Candida species. Candida spp. cause candidiasis by many virulence factors such as esterase, hemolysin and phospholipase.

OBJECTIVES: This study aimed to compare esterase and hemolytic activity in various Candida species isolated from oral cavity of diabetic and non-diabetic individuals.

PATIENTS AND METHODS: Swab samples were taken from 95 patients with diabetes (35 men and 60 women) and 95 normal persons (42 men and 53 women) and cultured on Sabouraud dextrose agar. Identification of isolated yeasts was performed by germ tube test, morphology on CHROMagar Candida medium, corn meal agar and ability to grow at 45°C. Hemolysin activity was evaluated using blood plate assay and esterase activity was determined using the Tween 80 opacity test.

RESULTS: Different Candida species were isolated from 57 (60%) diabetic and 24 (25%) non-diabetic individuals. Esterase activity was detected in all Candida isolates. Only 21.6% of C. albicans from patients with diabetes had esterase activity as + 3, while it ranged from + 1 to + 2 in others. Hemolytic activity was determined in C. albicans, C. dubliniensis, C. glabrata and C. krusei as 0.79, 0.58, 0.66 and 0.74, respectively. Hemolytic activity was significantly different in the two groups of diabetics and non-diabetics.

CONCLUSIONS: Oral carriage of C. albicans in the diabetic group (n = 42; 66.7%) was significantly greater than the control group (n = 16; 57.1%). Esterase activity of C. albicans in diabetic group was higher than non-diabetic group. Although C. albicans remains the most frequently pathogenic yeast for human, but other species are increasing.

Keywords: Candida albicans; Diabetes Mellitus; Esterases; Hemolysin Factor

References

  1. J Med Microbiol. 2007 Oct;56(Pt 10):1393-8 - PubMed
  2. J Oral Pathol Med. 2000 Feb;29(2):86-90 - PubMed
  3. Diabet Med. 2006 May;23(5):455-9 - PubMed
  4. Rev Iberoam Micol. 2006 Jun;23(2):101-3 - PubMed
  5. Sabouraudia. 1982 Mar;20(1):7-14 - PubMed
  6. J Int Med Res. 2002 May-Jun;30(3):322-4 - PubMed
  7. Clin Microbiol Rev. 2000 Oct;13(4):547-58 - PubMed
  8. J Clin Microbiol. 2000 Dec;38(12):4626-8 - PubMed
  9. Med Mycol. 2013 Aug;51(6):657-63 - PubMed
  10. Microbiol Rev. 1978 Mar;42(1):45-66 - PubMed
  11. Oral Microbiol Immunol. 2002 Jun;17(3):181-5 - PubMed
  12. Mycoses. 1996 May-Jun;39(5-6):161-7 - PubMed
  13. Infect Immun. 1994 Nov;62(11):5154-6 - PubMed
  14. Lancet. 2009 May 23;373(9677):1735 - PubMed
  15. Mycoses. 2005 Nov;48(6):365-77 - PubMed
  16. Mycoses. 2005 May;48(3):192-6 - PubMed
  17. J Clin Microbiol. 2001 Aug;39(8):2971-4 - PubMed

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