Display options
Cite
Cocca E, Petraccioli A, Morescalchi MA, et al. Laser microdissection-based analysis of the Y sex chromosome of the Antarctic fish Chionodracohamatus (Notothenioidei, Channichthyidae). Comp Cytogenet. 2015;9(1):1-15doi: 10.3897/CompCytogen.v9i1.8731.
Cocca, E., Petraccioli, A., Morescalchi, M. A., Odierna, G., & Capriglione, T. (2015). Laser microdissection-based analysis of the Y sex chromosome of the Antarctic fish Chionodracohamatus (Notothenioidei, Channichthyidae). Comparative cytogenetics, 9(1), 1-15. https://doi.org/10.3897/CompCytogen.v9i1.8731
Cocca, Ennio, et al. "Laser microdissection-based analysis of the Y sex chromosome of the Antarctic fish Chionodracohamatus (Notothenioidei, Channichthyidae)." Comparative cytogenetics vol. 9,1 (2015): 1-15. doi: https://doi.org/10.3897/CompCytogen.v9i1.8731
Cocca E, Petraccioli A, Morescalchi MA, Odierna G, Capriglione T. Laser microdissection-based analysis of the Y sex chromosome of the Antarctic fish Chionodracohamatus (Notothenioidei, Channichthyidae). Comp Cytogenet. 2015 Feb 05;9(1):1-15. doi: 10.3897/CompCytogen.v9i1.8731. eCollection 2015. PMID: 25893071; PMCID: PMC4387377.
Copy Download .nbib Format: NLM
Share it on

Comp Cytogenet. 2015 Feb 05;9(1):1-15. doi: 10.3897/CompCytogen.v9i1.8731. eCollection 2015.

Laser microdissection-based analysis of the Y sex chromosome of the Antarctic fish Chionodracohamatus (Notothenioidei, Channichthyidae).

Comparative cytogenetics

Ennio Cocca, Agnese Petraccioli, Maria Alessandra Morescalchi, Gaetano Odierna, Teresa Capriglione

Affiliations

  1. Istituto di Bioscienze e Biorisorse, CNR, via P. Castellino 111, 80131 Napoli, Italy.
  2. Dipartimento di Biologia, Università di Napoli Federico II, Complesso Universitario Monte S. Angelo, via Cinthia, 80126 Napoli, Italy.

PMID: 25893071 PMCID: PMC4387377 DOI: 10.3897/CompCytogen.v9i1.8731

Abstract

Microdissection, DOP-PCR amplification and microcloning were used to study the large Y chromosome of Chionodracohamatus, an Antarctic fish belonging to the Notothenioidei, the dominant component of the Southern Ocean fauna. The species has evolved a multiple sex chromosome system with digametic males showing an X1YX2 karyotype and females an X1X1X2X2 karyotype. Fluorescence in situ hybridization, performed with a painting probe made from microdissected Y chromosomes, allowed a deeper insight on the chromosomal rearrangement, which underpinned the fusion event that generated the Y. Then, we used a DNA library established by microdissection and microcloning of the whole Y chromosome of Chionodracohamatus for searching sex-linked sequences. One clone provided preliminary information on the presence on the Y chromosome of the CHD1 gene homologue, which is sex-linked in birds but in no other vertebrates. Several clones from the Y-chromosome mini-library contained microsatellites and transposable elements, one of which mapped to the q arm putative fusion region of the Y chromosome. The findings confirm that interspersed repetitive sequences might have fostered chromosome rearrangements and the emergence of the Y chromosome in Chionodracohamatus. Detection of the CHD1 gene in the Y sex-determining region could be a classical example of convergent evolution in action.

Keywords: Antarctic fish; CHD1 gene; Sex chromosomes

References

  1. Chromosome Res. 2011 Oct;19(7):843-55 - PubMed
  2. Cytogenet Genome Res. 2002;99(1-4):170-7 - PubMed
  3. Genetics. 2000 Aug;155(4):1903-12 - PubMed
  4. Mol Phylogenet Evol. 2011 Mar;58(3):439-46 - PubMed
  5. Cytogenet Genome Res. 2009;127(2-4):224-31 - PubMed
  6. Mar Genomics. 2009 Mar;2(1):75-80 - PubMed
  7. BMC Res Notes. 2011 Apr 12;4:119 - PubMed
  8. Cytogenet Cell Genet. 1999;87(3-4):278-81 - PubMed
  9. Cytogenet Genome Res. 2008;122(2):163-8 - PubMed
  10. Annu Rev Genet. 2005;39:293-307 - PubMed
  11. Genomics. 1992 Jul;13(3):718-25 - PubMed
  12. Chromosome Res. 1997 Dec;5(8):527-36 - PubMed
  13. Chromosoma. 1981;82(2):205-16 - PubMed
  14. Chromosome Res. 2012 Jan;20(1):95-110 - PubMed
  15. Gene. 2002 Aug 7;295(2):193-8 - PubMed
  16. Dev Dyn. 2013 Apr;242(4):339-53 - PubMed
  17. Proc Natl Acad Sci U S A. 2006 Nov 28;103(48):18190-5 - PubMed
  18. Comp Cytogenet. 2014 Jul 14;8(2):139-51 - PubMed
  19. Chromosome Res. 2000;8(4):335-40 - PubMed
  20. Am J Primatol. 1997;42(4):289-98 - PubMed
  21. Science. 1999 Oct 29;286(5441):964-7 - PubMed
  22. Trends Genet. 2003 Apr;19(4):196-9 - PubMed
  23. Genome. 2008 May;51(5):350-6 - PubMed
  24. Genome Biol Evol. 2014 Sep;6(9):2567-85 - PubMed
  25. Chromosome Res. 2006;14(3):283-96 - PubMed
  26. Biotechniques. 1999 Aug;27(2):362-7 - PubMed
  27. Genome. 2008 Sep;51(9):739-48 - PubMed
  28. Cytogenet Genome Res. 2007;116(1-2):132-4 - PubMed
  29. Nature. 2007 Apr 5;446(7136):672-5 - PubMed
  30. BMC Genet. 2011 Oct 20;12:90 - PubMed
  31. Cytogenet Genome Res. 2012;136(2):83-8 - PubMed
  32. J Fish Biol. 2012 May;80(6):2125-39 - PubMed
  33. Hum Genet. 1986 Aug;73(4):301-3 - PubMed
  34. Curr Genomics. 2007 Mar;8(1):67-72 - PubMed
  35. Curr Biol. 2004 Sep 21;14(18):R745-7 - PubMed
  36. Proc Natl Acad Sci U S A. 1992 Sep 15;89(18):8611-5 - PubMed
  37. Genetics. 2004 May;167(1):367-76 - PubMed
  38. BMC Genet. 2011 Jul 25;12:65 - PubMed
  39. Proc Natl Acad Sci U S A. 2002 Sep 3;99(18):11778-83 - PubMed
  40. Trends Genet. 2004 Jan;20(1):9-14 - PubMed
  41. Cytogenet Genome Res. 2002;98(4):285-90 - PubMed
  42. Nature. 2005 Mar 17;434(7031):325-37 - PubMed
  43. Chromosome Res. 2012 Jan;20(1):57-69 - PubMed
  44. PLoS One. 2012;7(4):e34397 - PubMed
  45. Genetics. 2010 Sep;186(1):9-31 - PubMed
  46. Heredity (Edinb). 2000 Dec;85(Pt 6):610-6 - PubMed
  47. Heredity (Edinb). 2005 Aug;95(2):118-28 - PubMed
  48. Gene. 2002 Aug 7;295(2):247-54 - PubMed
  49. Cytogenet Genome Res. 2011;132(4):289-96 - PubMed
  50. Sex Dev. 2009;3(2-3):88-98 - PubMed
  51. Chromosoma. 2011 Oct;120(5):455-68 - PubMed
  52. Mol Biol Evol. 2009 May;26(5):1017-27 - PubMed
  53. Cell. 2006 Mar 10;124(5):901-14 - PubMed
  54. Proc Natl Acad Sci U S A. 1995 Jul 18;92(15):6723-7 - PubMed

Publication Types