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Forneris NS, Otero G, Pereyra A, et al. High chromosomal variation in wild horn fly Haematobiairritans (Linnaeus) (Diptera, Muscidae) populations. Comp Cytogenet. 2015;9(1):31-50doi: 10.3897/CompCytogen.v9i1.8535.
Forneris, N. S., Otero, G., Pereyra, A., Repetto, G., Rabossi, A., Quesada-Allué, L. A., & Basso, A. L. (2015). High chromosomal variation in wild horn fly Haematobiairritans (Linnaeus) (Diptera, Muscidae) populations. Comparative cytogenetics, 9(1), 31-50. https://doi.org/10.3897/CompCytogen.v9i1.8535
Forneris, Natalia S, et al. "High chromosomal variation in wild horn fly Haematobiairritans (Linnaeus) (Diptera, Muscidae) populations." Comparative cytogenetics vol. 9,1 (2015): 31-50. doi: https://doi.org/10.3897/CompCytogen.v9i1.8535
Forneris NS, Otero G, Pereyra A, Repetto G, Rabossi A, Quesada-Allué LA, Basso AL. High chromosomal variation in wild horn fly Haematobiairritans (Linnaeus) (Diptera, Muscidae) populations. Comp Cytogenet. 2015 Feb 05;9(1):31-50. doi: 10.3897/CompCytogen.v9i1.8535. eCollection 2015. PMID: 25893073; PMCID: PMC4387379.
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Comp Cytogenet. 2015 Feb 05;9(1):31-50. doi: 10.3897/CompCytogen.v9i1.8535. eCollection 2015.

High chromosomal variation in wild horn fly Haematobiairritans (Linnaeus) (Diptera, Muscidae) populations.

Comparative cytogenetics

Natalia S Forneris, Gabriel Otero, Ana Pereyra, Gustavo Repetto, Alejandro Rabossi, Luis A Quesada-Allué, Alicia L Basso

Affiliations

  1. Cátedra de Genética, Facultad de Agronomía, Universidad de Buenos Aires, Av. San Martín 4453, C1417DSE CABA, Argentina.
  2. Departamento de Producción Animal, Facultad de Agronomía, Universidad de Buenos Aires, Av. San Martín 4453, C1417DSE CABA, Argentina.
  3. IIBBA-CONICET, Fundación Instituto Leloir and Dept. Biological Chemistry, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Patricias Argentinas 435, C1405BWE CABA, Argentina.

PMID: 25893073 PMCID: PMC4387379 DOI: 10.3897/CompCytogen.v9i1.8535

Abstract

The horn fly, Haematobiairritans is an obligate haematophagous cosmopolitan insect pest. The first reports of attacks on livestock by Haematobiairritans in Argentina and Uruguay occurred in 1991, and since 1993 it is considered an economically important pest. Knowledge on the genetic characteristics of the horn fly increases our understanding of the phenotypes resistant to insecticides that repeatedly develop in these insects. The karyotype of Haematobiairritans, as previously described using flies from an inbred colony, shows a chromosome complement of 2n=10 without heterochromosomes (sex chromosomes). In this study, we analyze for the first time the chromosome structure and variation of four wild populations of Haematobiairritans recently established in the Southern Cone of South America, collected in Argentina and Uruguay. In these wild type populations, we confirmed and characterized the previously published "standard" karyotype of 2n=10 without sex chromosomes; however, surprisingly a supernumerary element, called B-chromosome, was found in about half of mitotic preparations. The existence of statistically significant karyotypic diversity was demonstrated through the application of orcein staining, C-banding and H-banding. This study represents the first discovery and characterization of horn fly karyotypes with 2n=11 (2n=10+B). All spermatocytes analyzed showed 5 chromosome bivalents, and therefore, 2n=10 without an extra chromosome. Study of mitotic divisions showed that some chromosomal rearrangements affecting karyotype structure are maintained as polymorphisms, and multiple correspondence analyses demonstrated that genetic variation was not associated with geographic distribution. Because it was never observed during male meiosis, we hypothesize that B-chromosome is preferentially transmitted by females and that it might be related to sex determination.

Keywords: B-chromosome; H-banding; Karyotypes; chromosomal rearrangements; evolution; genetic variability; population structure

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