Display options
Cite
Lombardi B, Rendell N, Edwards M, et al. Evaluation of phosphopeptide enrichment strategies for quantitative TMT analysis of complex network dynamics in cancer-associated cell signalling. EuPA Open Proteom. 2015;6:10-15doi: 10.1016/j.euprot.2015.01.002.
Lombardi, B., Rendell, N., Edwards, M., Katan, M., & Zimmermann, J. G. (2015). Evaluation of phosphopeptide enrichment strategies for quantitative TMT analysis of complex network dynamics in cancer-associated cell signalling. EuPA open proteomics, 610-15. https://doi.org/10.1016/j.euprot.2015.01.002
Lombardi, Benedetta, et al. "Evaluation of phosphopeptide enrichment strategies for quantitative TMT analysis of complex network dynamics in cancer-associated cell signalling." EuPA open proteomics vol. 6 (2015): 10-15. doi: https://doi.org/10.1016/j.euprot.2015.01.002
Lombardi B, Rendell N, Edwards M, Katan M, Zimmermann JG. Evaluation of phosphopeptide enrichment strategies for quantitative TMT analysis of complex network dynamics in cancer-associated cell signalling. EuPA Open Proteom. 2015 Mar 01;6:10-15. doi: 10.1016/j.euprot.2015.01.002. PMID: 25893165; PMCID: PMC4398868.
Copy Download .nbib Format: NLM
Share it on

EuPA Open Proteom. 2015 Mar 01;6:10-15. doi: 10.1016/j.euprot.2015.01.002.

Evaluation of phosphopeptide enrichment strategies for quantitative TMT analysis of complex network dynamics in cancer-associated cell signalling.

EuPA open proteomics

Benedetta Lombardi, Nigel Rendell, Mina Edwards, Matilda Katan, Jasminka Godovac Zimmermann

Affiliations

  1. Proteomics and Molecular Cell Dynamics, Center for Nephrology, School of Life and Medical Sciences, University College London, Royal Free Campus, Rowland Hill Street, London NW3 2PF, United Kingdom.
  2. Wolfson Drug Discovery Unit, Centre for Amyloidosis and Acute Phase Proteins, Division of Medicine, University College London, London NW3 2PF, United Kingdom.
  3. Institute of Structural and Molecular Biology, Division of Biosciences, University College London, Gower Street, London WC1E 6BT, United Kingdom.

PMID: 25893165 PMCID: PMC4398868 DOI: 10.1016/j.euprot.2015.01.002

Abstract

Defining alterations in signalling pathways in normal and malignant cells is becoming a major field in proteomics. A number of different approaches have been established to isolate, identify and quantify phosphorylated proteins and peptides. In the current report, a comparison between SCX prefractionation

Keywords: Peptides immunoprecipitation; Phosphopeptides enrichment; TMT; Titanium dioxide

References

  1. Hum Mol Genet. 2013 Feb 15;22(4):795-803 - PubMed
  2. J Proteome Res. 2013 Apr 5;12(4):1856-67 - PubMed
  3. Cell. 2006 Nov 3;127(3):635-48 - PubMed
  4. J Proteome Res. 2014 Dec 5;13(12):5293-309 - PubMed
  5. Anal Biochem. 2008 Nov 15;382(2):135-7 - PubMed
  6. Mol Cell Proteomics. 2009 Feb;8(2):226-31 - PubMed
  7. Mol Cell Proteomics. 2011 Dec;10(12):M111.009910 - PubMed
  8. Mol Cancer Ther. 2003 Dec;2(12):1369-77 - PubMed
  9. Biochem J. 2013 Sep 15;454(3):501-13 - PubMed
  10. Nat Protoc. 2008;3(10):1630-8 - PubMed
  11. Anal Chem. 2004 Jul 1;76(13):3590-8 - PubMed
  12. Proc Natl Acad Sci U S A. 2004 Jun 29;101(26):9528-33 - PubMed
  13. Mol Cell Proteomics. 2009 Dec;8(12):2759-69 - PubMed
  14. Nature. 2005 Dec 1;438(7068):679-84 - PubMed
  15. Trends Biotechnol. 2002 Jun;20(6):261-8 - PubMed
  16. Anal Chem. 2010 Aug 1;82(15):6549-58 - PubMed
  17. Proteomics. 2010 Jun;10(11):2224-34 - PubMed
  18. Proteomics. 2013 Mar;13(6):910-31 - PubMed
  19. J Proteome Res. 2009 Oct;8(10):4665-75 - PubMed
  20. Nat Rev Cancer. 2010 Feb;10(2):116-29 - PubMed
  21. Mol Biosyst. 2010 Jul;6(7):1227-37 - PubMed
  22. Cancer Res. 2008 Sep 15;68(18):7270-7 - PubMed
  23. Mol Cell Proteomics. 2012 Dec;11(12):1724-40 - PubMed
  24. J Proteome Res. 2014 Dec 5;13(12):6176-86 - PubMed
  25. J Proteome Res. 2012 Jul 6;11(7):3774-81 - PubMed
  26. Proc Natl Acad Sci U S A. 1980 Mar;77(3):1311-5 - PubMed

Publication Types

Grant support