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Papoudou-Bai A, Barbouti A, Galani V, et al. Immunohistological analysis of the jun family and the signal transducers and activators of transcription in thymus. Anat Res Int. 2015;2015:541582doi: 10.1155/2015/541582.
Papoudou-Bai, A., Barbouti, A., Galani, V., Stefanaki, K., & Kanavaros, P. (2015). Immunohistological analysis of the jun family and the signal transducers and activators of transcription in thymus. Anatomy research international, 2015541582. https://doi.org/10.1155/2015/541582
Papoudou-Bai, Alexandra, et al. "Immunohistological analysis of the jun family and the signal transducers and activators of transcription in thymus." Anatomy research international vol. 2015 (2015): 541582. doi: https://doi.org/10.1155/2015/541582
Papoudou-Bai A, Barbouti A, Galani V, Stefanaki K, Kanavaros P. Immunohistological analysis of the jun family and the signal transducers and activators of transcription in thymus. Anat Res Int. 2015;2015:541582. doi: 10.1155/2015/541582. Epub 2015 Mar 18. PMID: 25866678; PMCID: PMC4381968.
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Anat Res Int. 2015;2015:541582. doi: 10.1155/2015/541582. Epub 2015 Mar 18.

Immunohistological analysis of the jun family and the signal transducers and activators of transcription in thymus.

Anatomy research international

Alexandra Papoudou-Bai, Alexandra Barbouti, Vassiliki Galani, Kalliopi Stefanaki, Panagiotis Kanavaros

Affiliations

  1. Department of Pathology, University of Ioannina, 45110 Ioannina, Greece ; Department of Anatomy-Histology-Embryology, University of Ioannina, 45110 Ioannina, Greece.
  2. Department of Anatomy-Histology-Embryology, University of Ioannina, 45110 Ioannina, Greece.
  3. Department of Pathology, Agia Sofia Children's Hospital of Athens, 11527 Athens, Greece.

PMID: 25866678 PMCID: PMC4381968 DOI: 10.1155/2015/541582

Abstract

The Jun family and the signal transducers and activators of transcription (STAT) are involved in proliferation and apoptosis. Moreover, c-Jun and STAT3 cooperate to regulate apoptosis. Therefore, we used double immunostaining to investigate the immunotopographical distribution of phospho-c-Jun (p-c-Jun), JunB, JunD, p-STAT3, p-STAT5, and p-STAT6 in human thymus. JunD was frequently expressed by thymocytes with higher expression in medullary compared to cortical thymocytes. p-c-Jun was frequently expressed by cortical and medullary thymic epithelial cells (TEC) and Hassall bodies (HB). p-STAT3 was frequently expressed by TEC with higher expression in cortical compared to medullary TEC and HB. p-c-Jun, JunB, p-STAT3, p-STAT5, and p-STAT6 were rarely expressed by thymocytes. JunB and JunD were expressed by rare cortical TEC with higher expression in medullary TEC. p-STAT5 and p-STAT6 were expressed by rare cortical and medullary TEC. Double immunostaining revealed p-c-Jun and JunD expression in rare CD11c positive dendritic cells. Our findings suggest a notable implication of JunD in the physiology of thymocytes and p-c-Jun and p-STAT3 in the physiology of TEC. The diversity of the immunotopographical distribution and the expression levels of p-c-Jun, JunB, JunD, p-STAT3, p-STAT5, and p-STAT6 indicates that they are differentially involved in the differentiation of TEC, thymocytes, and dendritic cells.

References

  1. Histochem Cell Biol. 2000 Jun;113(6):427-31 - PubMed
  2. Curr Opin Immunol. 2010 Oct;22(5):592-600 - PubMed
  3. Biochem Biophys Res Commun. 2011 May 20;408(4):530-6 - PubMed
  4. Mol Cell. 2001 Mar;7(3):517-28 - PubMed
  5. Biomed Res Int. 2014;2014:206929 - PubMed
  6. EMBO J. 2004 Mar 24;23 (6):1325-35 - PubMed
  7. Prog Histochem Cytochem. 2008;43(2):73-120 - PubMed
  8. Am J Surg Pathol. 2007 Mar;31(3):447-53 - PubMed
  9. Biochem Soc Trans. 2008 Oct;36(Pt 5):864-7 - PubMed
  10. Curr Opin Immunol. 2011 Apr;23(2):190-7 - PubMed
  11. J Dermatol Sci. 2006 Apr;42(1):1-11 - PubMed
  12. Histopathology. 2000 May;36(5):466-9 - PubMed
  13. Cell Signal. 2003 Jun;15(6):625-36 - PubMed
  14. J Cell Physiol. 2003 Nov;197(2):157-68 - PubMed
  15. Curr Opin Cell Biol. 2010 Dec;22(6):865-71 - PubMed
  16. Exp Cell Res. 1997 Feb 1;230(2):220-32 - PubMed
  17. Histol Histopathol. 2001 Oct;16(4):1005-12 - PubMed
  18. Ann Anat. 2013 Mar;195(2):159-65 - PubMed
  19. J Clin Invest. 2007 May;117(5):1175-83 - PubMed
  20. EMBO J. 2002 Aug 1;21(15):4104-13 - PubMed
  21. Immunol Res. 2012 Dec;54(1-3):177-90 - PubMed
  22. Ann Anat. 2006 Jul;188(4):345-52 - PubMed
  23. Mol Immunol. 2013 Mar;53(3):295-301 - PubMed
  24. Immunol Lett. 2010 Aug 16;132(1-2):6-11 - PubMed
  25. Histopathology. 1997 Jun;30(6):549-55 - PubMed
  26. Mol Cell. 2003 Jun;11(6):1479-89 - PubMed
  27. Oncogene. 2008 Aug 14;27(35):4757-67 - PubMed
  28. Clin Immunol. 2008 Jan;126(1):1-12 - PubMed
  29. Int J Cancer. 2010 Feb 15;126(4):819-29 - PubMed
  30. Cytokine Growth Factor Rev. 2000 Sep;11(3):199-207 - PubMed
  31. J Clin Invest. 2002 May;109(9):1139-42 - PubMed
  32. Trends Immunol. 2009 Oct;30(10):468-74 - PubMed
  33. Trends Immunol. 2012 Jun;33(6):256-63 - PubMed
  34. J Cardiothorac Surg. 2013 Apr 16;8:92 - PubMed
  35. Mol Cell Biol. 1996 Mar;16(3):1074-84 - PubMed
  36. Blood. 2003 Dec 1;102(12):4159-65 - PubMed
  37. Vet Immunol Immunopathol. 2004 Jun;99(3-4):203-13 - PubMed
  38. Mol Cell. 2000 Nov;6(5):1109-19 - PubMed
  39. Cell Signal. 2010 Jun;22(6):894-9 - PubMed
  40. J Pathol. 2006 Oct;210(2):224-33 - PubMed
  41. Acta Histochem. 2001 Feb;103(1):89-98 - PubMed
  42. Adv Immunol. 2008;99:59-94 - PubMed
  43. Diagn Pathol. 2012 Sep 14;7:120 - PubMed
  44. Mod Pathol. 2005 Oct;18(10):1365-70 - PubMed
  45. Immunology. 2004 Jun;112(2):191-200 - PubMed
  46. Cytokine Growth Factor Rev. 2002 Aug-Oct;13(4-5):413-21 - PubMed
  47. Pediatr Dev Pathol. 2002 Sep-Oct;5(5):465-71 - PubMed
  48. Immunology. 2005 Mar;114(3):301-12 - PubMed
  49. Anticancer Res. 2008 Jan-Feb;28(1A):295-303 - PubMed
  50. Ann Anat. 2005 Apr;187(2):175-7 - PubMed
  51. In Vivo. 2012 Nov-Dec;26(6):985-92 - PubMed
  52. Am J Clin Pathol. 2005 Jul;124(1):11-9 - PubMed
  53. Autophagy. 2010 May;6(4):566-7 - PubMed
  54. Histopathology. 2014 Mar;64(4):557-66 - PubMed
  55. Immunity. 2001 Aug;15(2):261-73 - PubMed
  56. Acta Histochem. 1999 Nov;101(4):421-9 - PubMed
  57. Autophagy. 2013 Jun 1;9(6):931-2 - PubMed

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