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Chang HJ, Li TF, Guo JL, et al. Effects of high glucose on expression of OPG and RANKL in rat aortic vascular smooth muscle cells. Asian Pac J Trop Med. 2015;8(3):209-13doi: 10.1016/S1995-7645(14)60317-5.
Chang, H. J., Li, T. F., Guo, J. L., Lan, Y. L., Kong, Y. Q., Meng, X., Ma, X. J., Lu, X. L., Lu, W. Y., & Zheng, S. J. (2015). Effects of high glucose on expression of OPG and RANKL in rat aortic vascular smooth muscle cells. Asian Pacific journal of tropical medicine, 8(3), 209-13. https://doi.org/10.1016/S1995-7645(14)60317-5
Chang, Hong-Juan, et al. "Effects of high glucose on expression of OPG and RANKL in rat aortic vascular smooth muscle cells." Asian Pacific journal of tropical medicine vol. 8,3 (2015): 209-13. doi: https://doi.org/10.1016/S1995-7645(14)60317-5
Chang HJ, Li TF, Guo JL, Lan YL, Kong YQ, Meng X, Ma XJ, Lu XL, Lu WY, Zheng SJ. Effects of high glucose on expression of OPG and RANKL in rat aortic vascular smooth muscle cells. Asian Pac J Trop Med. 2015 Mar;8(3):209-13. doi: 10.1016/S1995-7645(14)60317-5. PMID: 25902163.
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Asian Pac J Trop Med. 2015 Mar;8(3):209-13. doi: 10.1016/S1995-7645(14)60317-5.

Effects of high glucose on expression of OPG and RANKL in rat aortic vascular smooth muscle cells.

Asian Pacific journal of tropical medicine

Hong-Juan Chang, Tian-Fa Li, Jun-Li Guo, You-Ling Lan, Yue-Qiong Kong, Xin Meng, Xian-Ji Ma, Xiao-Ling Lu, Wei-Ying Lu, Shao-Jiang Zheng

Affiliations

  1. Department of Neurology, the Second People's Hospital of Xinxiang, Xinxiang 453002, PR China.
  2. Department of Cardiovascular Institute, Affiliated Hospital of Hainan Medical College, Haikou 571199, PR China.
  3. National Center for International Research of Biological Targeting Diagnosis and Therapy, Guangxi Key Laboratory of Biological Targeting Diagnosis and Therapy Research, Guangxi Medical University, Nanning 530021, PR China.
  4. Reproductive Medicine Center, Affiliated Hospital of Hainan Medical College, Haikou 570102, PR China.
  5. Department of Pathology, Affiliated Hospital of Hainan Medical College, Haikou 570102, PR China.

PMID: 25902163 DOI: 10.1016/S1995-7645(14)60317-5

Abstract

OBJECTIVE: To explore effect of high glucose on expression of osteoprotegerin (OPG) and receptor activator of NF- κ B ligand (RANKE) in rat aortic vascular smooth muscle cells.

METHODS: SD rats were intraperitoneally injected with streptozotocin, OPG and RANKL expression in rat thoracic aortas were detected by immunohistochemical staining. In cultured vascular smooth muscle cells (VSMCs) (A7r5), qRT-PCR and Western blot analysis were used to examine the mRNA and protein levels of OPG and RANKL.

RESULTS: Our results demonstrated that OPG expression was increased in hyperglycemic rat aortic VSMCs, while RANKL expression was decreased. Besides, in vitro experiments high glucose induced OPG expression, but depressed RANKL expression by dose- and time-dependent manner in cultured A7r5.

CONCLUSIONS: Our findings suggested that high glucose could promote the expression of OPG, and inhibit the expression of RANKL in VSMCs, which may be partly be the molecular mechanism of diabetic vascular calcification.

Copyright © 2015 Hainan Medical College. Production and hosting by Elsevier B.V. All rights reserved.

Keywords: High glucose; Osteoprotegerin; Receptor activator of NF– κ B ligand; Vascular smooth muscle cells

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