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Pringle KG, Wang Y, Lumbers ER. The synthesis, secretion and uptake of prorenin in human amnion. Physiol Rep. 2015;3(4)doi: 10.14814/phy2.12313.
Pringle, K. G., Wang, Y., & Lumbers, E. R. (2015). The synthesis, secretion and uptake of prorenin in human amnion. Physiological reports, 3(4), . https://doi.org/10.14814/phy2.12313
Pringle, Kirsty G, et al. "The synthesis, secretion and uptake of prorenin in human amnion." Physiological reports vol. 3,4 (2015). doi: https://doi.org/10.14814/phy2.12313
Pringle KG, Wang Y, Lumbers ER. The synthesis, secretion and uptake of prorenin in human amnion. Physiol Rep. 2015 Apr;3(4). doi: 10.14814/phy2.12313. PMID: 25902786; PMCID: PMC4425950.
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Physiol Rep. 2015 Apr;3(4). doi: 10.14814/phy2.12313.

The synthesis, secretion and uptake of prorenin in human amnion.

Physiological reports

Kirsty G Pringle, Yu Wang, Eugenie R Lumbers

Affiliations

  1. Mothers and Babies Research Centre, Hunter Medical Research Institute, John Hunter Hospital & School of Biomedical Sciences and Pharmacy, University of Newcastle, Newcastle, New South Wales, Australia [email protected].
  2. Mothers and Babies Research Centre, Hunter Medical Research Institute, John Hunter Hospital & School of Biomedical Sciences and Pharmacy, University of Newcastle, Newcastle, New South Wales, Australia.

PMID: 25902786 PMCID: PMC4425950 DOI: 10.14814/phy2.12313

Abstract

Very high concentrations of prorenin protein occur in human amniotic fluid and amnion. The source of amniotic fluid prorenin is likely the decidua, as it has the highest levels of prorenin mRNA (REN). Conversely, REN mRNA levels in amnion and chorion are very low. This study aimed to investigate whether decidual prorenin could cross the amnion and accumulate in amniotic fluid. Late gestation amnion was incubated for 24 h in the presence or absence of recombinant human (rh)prorenin. REN mRNA abundance was determined by qPCR and prorenin protein levels in the supernatant and tissue were measured by an ELISA. Prior to incubation only 3/11 amnion samples had REN mRNA but measurable levels of prorenin protein were found (1.4 ng/mg total protein). After 24 h incubation, REN mRNA was found in all explants and levels were significantly increased (P = 0.03) but prorenin protein levels in amnion were unchanged. Prorenin protein levels in the supernatant were, however, increased (P = 0.048). Incubation with (rh)prorenin significantly increased amnion tissue prorenin levels (2.8 ng/mg total protein, P = 0.001); REN mRNA levels were unchanged. Therefore, amnion explants express small amounts of REN and secrete prorenin protein. Prorenin is also taken up by amnion. We postulate that the amniotic renin angiotensin system (RAS) alters pregnancy outcome through effects on gestation length and amniotic fluid volume. Since human amnion can take up and secrete prorenin protein, the activity of both amnion and amniotic fluid RASs can be amplified by prorenin produced by other intrauterine tissues.

© 2015 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.

Keywords: Amnion; amniotic fluid; human pregnancy; pregnancy outcome; prorenin

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