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Nieman DC, Shanely RA, Zwetsloot KA, et al. Ultrasonic assessment of exercise-induced change in skeletal muscle glycogen content. BMC Sports Sci Med Rehabil. 2015;7:9doi: 10.1186/s13102-015-0003-z.
Nieman, D. C., Shanely, R. A., Zwetsloot, K. A., Meaney, M. P., & Farris, G. E. (2015). Ultrasonic assessment of exercise-induced change in skeletal muscle glycogen content. BMC sports science, medicine & rehabilitation, 79. https://doi.org/10.1186/s13102-015-0003-z
Nieman, David C, et al. "Ultrasonic assessment of exercise-induced change in skeletal muscle glycogen content." BMC sports science, medicine & rehabilitation vol. 7 (2015): 9. doi: https://doi.org/10.1186/s13102-015-0003-z
Nieman DC, Shanely RA, Zwetsloot KA, Meaney MP, Farris GE. Ultrasonic assessment of exercise-induced change in skeletal muscle glycogen content. BMC Sports Sci Med Rehabil. 2015 Apr 18;7:9. doi: 10.1186/s13102-015-0003-z. eCollection 2015. PMID: 25905021; PMCID: PMC4406335.
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BMC Sports Sci Med Rehabil. 2015 Apr 18;7:9. doi: 10.1186/s13102-015-0003-z. eCollection 2015.

Ultrasonic assessment of exercise-induced change in skeletal muscle glycogen content.

BMC sports science, medicine & rehabilitation

David C Nieman, R Andrew Shanely, Kevin A Zwetsloot, Mary Pat Meaney, Gerald E Farris

Affiliations

  1. Appalachian State University, Human Performance Lab, North Carolina Research Campus, 600 Laureate Way, Kannapolis, NC 28081 USA.
  2. Department of Health and Exercise Science, Appalachian State University, Boone, NC USA.
  3. Department of Emergency Medicine, Carolinas Medical Center NorthEast, Concord, NC USA.

PMID: 25905021 PMCID: PMC4406335 DOI: 10.1186/s13102-015-0003-z

Abstract

BACKGROUND: Ultrasound imaging is a valuable tool in exercise and sport science research, and has been used to visualize and track real-time movement of muscles and tendons, estimate hydration status in body tissues, and most recently, quantify skeletal muscle glycogen content. In this validation study, direct glycogen quantification from pre-and post-exercise muscle biopsy samples was compared with glycogen content estimates made through a portable, diagnostic high-frequency ultrasound and cloud-based software system (MuscleSound®, Denver, CO).

METHODS: Well-trained cyclists (N = 20, age 38.4 ± 6.0 y, 351 ± 57.6 wattsmax) participated in a 75-km cycling time trial on their own bicycles using CompuTrainer Pro Model 8001 trainers (RacerMate, Seattle, WA). Muscle biopsy samples and ultrasound measurements were acquired pre- and post-exercise. Specific locations on the vastus lateralis were marked, and a trained technician used a 12 MHz linear transducer and a standard diagnostic high resolution GE LOGIQ-e ultrasound machine (GE Healthcare, Milwaukee, WI) to make three ultrasound measurements. Ultrasound images were pre-processed to isolate the muscle area under analysis, with the mean pixel intensity averaged from the three scans and scaled (0 to 100 scale) to create the glycogen score. Pre- and post-exercise muscle biopsy samples were acquired at the vastus lateralis location (2 cm apart) using the suction-modified percutaneous needle biopsy procedure, and analyzed for glycogen content.

RESULTS: The 20 cyclists completed the 75-km cycling time trial in 168 ± 26.0 minutes at a power output of 193 ± 57.8 watts (54.2 ± 9.6% wattsmax). Muscle glycogen decreased 77.2 ± 17.4%, with an absolute change of 71.4 ± 23.1 mmol glycogen per kilogram of muscle. The MuscleSound® change score at the vastus lateralis site correlated highly with change in measured muscle glycogen content (R = 0.92, P < 0.001).

CONCLUSIONS: MuscleSound® change scores acquired from an average of three ultrasound scans at the vastus lateralis site correlated significantly with change in vastus lateralis muscle glycogen content. These data support the use of the MuscleSound® system for accurately and non-invasively estimating exercise-induced decreases in vastus lateralis skeletal muscle glycogen content.

Keywords: Cycling; Muscle biopsy; Skeletal muscle; Sonography; Vastus lateralis

References

  1. J Appl Physiol (1985). 1986 Aug;61(2):391-401 - PubMed
  2. Exerc Sport Sci Rev. 2014 Jul;42(3):126-35 - PubMed
  3. Exerc Sport Sci Rev. 1987;15:1-28 - PubMed
  4. Phys Sportsmed. 2014 Sep;42(3):45-52 - PubMed
  5. Am J Clin Nutr. 1993 Jan;57(1):27-31 - PubMed
  6. Med Sci Sports Exerc. 2005 Aug;37(8):1283-90 - PubMed
  7. J Strength Cond Res. 2010 Jun;24(6):1451-7 - PubMed
  8. Sports Med. 2012 Aug 1;42(8):665-80 - PubMed
  9. Ultrasonics. 2005 Aug;43(8):661-71 - PubMed
  10. Proc Natl Acad Sci U S A. 2007 Mar 13;104(11):4359-64 - PubMed
  11. Curr Radiol Rep. 2013 Jun 1;1(2):102-114 - PubMed
  12. Muscle Nerve. 2010 Jan;41(1):32-41 - PubMed
  13. J Vis Exp. 2014 Sep 10;(91):51812 - PubMed
  14. Ultrasonics. 2006 Jul;44(3):259-64 - PubMed
  15. Proc Natl Acad Sci U S A. 1988 Mar;85(5):1634-6 - PubMed
  16. Scand J Rehabil Med. 1995 Mar;27(1):51-8 - PubMed
  17. J Appl Physiol (1985). 2003 May;94(5):1917-25 - PubMed
  18. J Appl Physiol (1985). 2007 Dec;103(6):1986-98 - PubMed
  19. J Appl Physiol (1985). 2010 Sep;109 (3):906-15 - PubMed

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