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Tauriainen J, Gustafsson K, Göthlin M, et al. Single-Cell Characterization of in vitro Migration and Interaction Dynamics of T Cells Expanded with IL-2 and IL-7. Front Immunol. 2015;6:196doi: 10.3389/fimmu.2015.00196.
Tauriainen, J., Gustafsson, K., Göthlin, M., Gertow, J., Buggert, M., Frisk, T. W., Karlsson, A. C., Uhlin, M., & Önfelt, B. (2015). Single-Cell Characterization of in vitro Migration and Interaction Dynamics of T Cells Expanded with IL-2 and IL-7. Frontiers in immunology, 6196. https://doi.org/10.3389/fimmu.2015.00196
Tauriainen, Johanna, et al. "Single-Cell Characterization of in vitro Migration and Interaction Dynamics of T Cells Expanded with IL-2 and IL-7." Frontiers in immunology vol. 6 (2015): 196. doi: https://doi.org/10.3389/fimmu.2015.00196
Tauriainen J, Gustafsson K, Göthlin M, Gertow J, Buggert M, Frisk TW, Karlsson AC, Uhlin M, Önfelt B. Single-Cell Characterization of in vitro Migration and Interaction Dynamics of T Cells Expanded with IL-2 and IL-7. Front Immunol. 2015 Apr 28;6:196. doi: 10.3389/fimmu.2015.00196. eCollection 2015. PMID: 25972868; PMCID: PMC4412128.
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Front Immunol. 2015 Apr 28;6:196. doi: 10.3389/fimmu.2015.00196. eCollection 2015.

Single-Cell Characterization of in vitro Migration and Interaction Dynamics of T Cells Expanded with IL-2 and IL-7.

Frontiers in immunology

Johanna Tauriainen, Karin Gustafsson, Mårten Göthlin, Jens Gertow, Marcus Buggert, Thomas W Frisk, Annika C Karlsson, Michael Uhlin, Björn Önfelt

Affiliations

  1. Department of Laboratory Medicine, Division of Clinical Microbiology, Karolinska Institutet , Stockholm , Sweden.
  2. Science for Life Laboratory, Department of Applied Physics, KTH Royal Institute of Technology , Stockholm , Sweden.
  3. Center for Allogeneic Stem Cell Transplantation, Karolinska University Hospital Huddinge , Stockholm , Sweden ; Department of Oncology and Pathology, Karolinska Institutet , Stockholm , Sweden.
  4. Science for Life Laboratory, Department of Applied Physics, KTH Royal Institute of Technology , Stockholm , Sweden ; Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet , Stockholm , Sweden.

PMID: 25972868 PMCID: PMC4412128 DOI: 10.3389/fimmu.2015.00196

Abstract

T cells are pivotal in the immune defense against cancers and infectious agents. To mount an effector response against cancer cells, T cells need to migrate to the cancer-site, engage in contacts with cancer cells, and perform their effector functions. Adoptive T cell therapy is an effective strategy as treatment of complications such as relapse or opportunistic infections after hematopoietic stem cell transplantations. This requires a sufficient amount of cells that are able to expand and respond to tumor or viral antigens. The cytokines interleukin (IL)-2 and IL-7 drive T cell differentiation, proliferation, and survival and are commonly used to expand T cells ex vivo. Here, we have used microchip-based live-cell imaging to follow the migration of individual T cells, their interactions with allogeneic monocytes, cell division, and apoptosis for extended periods of time; something that cannot be achieved by commonly used methods. Our data indicate that cells grown in IL-7 + IL-2 had similar migration and contact dynamics as cells grown in IL-2 alone. However, the addition of IL-7 decreased cell death creating a more viable cell population, which should be beneficial when preparing cells for immunotherapy.

Keywords: IL-2; IL-7; T cell; fluorescence; live-cell imaging; microchip; microscopy; single-cell analysis

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