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Gonçalves AP, Cordeiro JM, Monteiro J, et al. Involvement of mitochondrial proteins in calcium signaling and cell death induced by staurosporine in Neurospora crassa. Biochim Biophys Acta. 2015;1847(10):1064-74doi: 10.1016/j.bbabio.2015.05.011.
Gonçalves, A. P., Cordeiro, J. M., Monteiro, J., Lucchi, C., Correia-de-Sá, P., & Videira, A. (2015). Involvement of mitochondrial proteins in calcium signaling and cell death induced by staurosporine in Neurospora crassa. Biochimica et biophysica acta, 1847(10), 1064-74. https://doi.org/10.1016/j.bbabio.2015.05.011
Gonçalves, A Pedro, et al. "Involvement of mitochondrial proteins in calcium signaling and cell death induced by staurosporine in Neurospora crassa." Biochimica et biophysica acta vol. 1847,10 (2015): 1064-74. doi: https://doi.org/10.1016/j.bbabio.2015.05.011
Gonçalves AP, Cordeiro JM, Monteiro J, Lucchi C, Correia-de-Sá P, Videira A. Involvement of mitochondrial proteins in calcium signaling and cell death induced by staurosporine in Neurospora crassa. Biochim Biophys Acta. 2015 Oct;1847(10):1064-74. doi: 10.1016/j.bbabio.2015.05.011. Epub 2015 May 22. PMID: 26004627.
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Biochim Biophys Acta. 2015 Oct;1847(10):1064-74. doi: 10.1016/j.bbabio.2015.05.011. Epub 2015 May 22.

Involvement of mitochondrial proteins in calcium signaling and cell death induced by staurosporine in Neurospora crassa.

Biochimica et biophysica acta

A Pedro Gonçalves, J Miguel Cordeiro, João Monteiro, Chiara Lucchi, Paulo Correia-de-Sá, Arnaldo Videira

Affiliations

  1. ICBAS-Instituto de Ciências Biomédicas de Abel Salazar, Universidade do Porto, Rua de Jorge Viterbo Ferreira 228, 4050-313 Porto, Portugal; IBMC-Instituto de Biologia Molecular e Celular, Universidade do Porto, Rua do Campo Alegre 823, 4150-180 Porto, Portugal. Electronic address: [email protected].
  2. ICBAS-Instituto de Ciências Biomédicas de Abel Salazar, Universidade do Porto, Rua de Jorge Viterbo Ferreira 228, 4050-313 Porto, Portugal.
  3. IBMC-Instituto de Biologia Molecular e Celular, Universidade do Porto, Rua do Campo Alegre 823, 4150-180 Porto, Portugal.
  4. ICBAS-Instituto de Ciências Biomédicas de Abel Salazar, Universidade do Porto, Rua de Jorge Viterbo Ferreira 228, 4050-313 Porto, Portugal; IBMC-Instituto de Biologia Molecular e Celular, Universidade do Porto, Rua do Campo Alegre 823, 4150-180 Porto, Portugal. Electronic address: [email protected].

PMID: 26004627 DOI: 10.1016/j.bbabio.2015.05.011

Abstract

Staurosporine-induced cell death in Neurospora crassa includes a well defined sequence of alterations in cytosolic calcium levels, comprising extracellular Ca(2+) influx and mobilization of Ca(2+) from internal stores. Here, we show that cells undergoing respiratory stress due to the lack of certain components of the mitochondrial complex I (like the 51kDa and 14kDa subunits) or the Ca(2+)-binding alternative NADPH dehydrogenase NDE-1 are hypersensitive to staurosporine and incapable of setting up a proper intracellular Ca(2+) response. Cells expressing mutant forms of NUO51 that mimic human metabolic diseases also presented Ca(2+) signaling deficiencies. Accumulation of reactive oxygen species is increased in cells lacking NDE-1 and seems to be required for Ca(2+) oscillations in response to staurosporine. Measurement of the mitochondrial levels of Ca(2+) further supported the involvement of these organelles in staurosporine-induced Ca(2+) signaling. In summary, our data indicate that staurosporine-induced fungal cell death involves a sophisticated response linking Ca(2+) dynamics and bioenergetics.

Copyright © 2015 Elsevier B.V. All rights reserved.

Keywords: Alternative NAD(P)H dehydrogenase; Ca(2+) dynamics; Cell death; Complex I; Mitochondrion; NDE-1

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