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Wagner R, Gabriel G, Schlesner M, et al. Protease activation mutants elicit protective immunity against highly pathogenic avian influenza viruses of subtype H7 in chickens and mice. Emerg Microbes Infect. 2013;2(2):e7doi: 10.1038/emi2013.7.
Wagner, R., Gabriel, G., Schlesner, M., Alex, N., Herwig, A., Werner, O., & Klenk, H. D. (2013). Protease activation mutants elicit protective immunity against highly pathogenic avian influenza viruses of subtype H7 in chickens and mice. Emerging microbes & infections, 2(2), e7. https://doi.org/10.1038/emi2013.7
Wagner, Ralf, et al. "Protease activation mutants elicit protective immunity against highly pathogenic avian influenza viruses of subtype H7 in chickens and mice." Emerging microbes & infections vol. 2,2 (2013): e7. doi: https://doi.org/10.1038/emi2013.7
Wagner R, Gabriel G, Schlesner M, Alex N, Herwig A, Werner O, Klenk HD. Protease activation mutants elicit protective immunity against highly pathogenic avian influenza viruses of subtype H7 in chickens and mice. Emerg Microbes Infect. 2013 Feb;2(2):e7. doi: 10.1038/emi2013.7. Epub 2013 Feb 27. PMID: 26038453; PMCID: PMC3630491.
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Emerg Microbes Infect. 2013 Feb;2(2):e7. doi: 10.1038/emi2013.7. Epub 2013 Feb 27.

Protease activation mutants elicit protective immunity against highly pathogenic avian influenza viruses of subtype H7 in chickens and mice.

Emerging microbes & infections

Ralf Wagner, Gülsah Gabriel, Matthias Schlesner, Nina Alex, Astrid Herwig, Ortrud Werner, Hans-Dieter Klenk

Affiliations

  1. Section Viral Vaccines, Paul-Ehrlich-Institut , 63225 Langen, Germany.
  2. Institute for Virology, Philipps-University Marburg , 35043 Marburg, Germany.
  3. Friedrich-Loeffler-Institute , Greifswald-Insel Riems, 17493 Greifswald-Insel Riems, Germany.

PMID: 26038453 PMCID: PMC3630491 DOI: 10.1038/emi2013.7

Abstract

Protease activation mutants of the highly pathogenic avian influenza virus A/FPV/Rostock/34 (H7N1) have been generated that are fully dependent on the presence of trypsin for growth in cell culture. Unlike wild-type virus, the mutants do not induce systemic infection in chicken embryos and show low pathogenicity in both chicken embryos and adult chickens. Inactivated vaccines prepared from the mutants protected chickens and mice very efficiently against infection with highly pathogenic wild-type virus in a cross-reactive manner. The potential of these mutants to be used as veterinary and prepandemic vaccines will be discussed.

Keywords: influenza virus; pathogenicity; protection; proteolytic activation; vaccine

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