Display options
Cite
Slabaugh MB, Cooper LD, Kishore VK, et al. Genes affecting novel seed constituents in Limnanthes alba Benth: transcriptome analysis of developing embryos and a new genetic map of meadowfoam. PeerJ. 2015;3:e915doi: 10.7717/peerj.915.
Slabaugh, M. B., Cooper, L. D., Kishore, V. K., Knapp, S. J., & Kling, J. G. (2015). Genes affecting novel seed constituents in Limnanthes alba Benth: transcriptome analysis of developing embryos and a new genetic map of meadowfoam. PeerJ, 3e915. https://doi.org/10.7717/peerj.915
Slabaugh, Mary B, et al. "Genes affecting novel seed constituents in Limnanthes alba Benth: transcriptome analysis of developing embryos and a new genetic map of meadowfoam." PeerJ vol. 3 (2015): e915. doi: https://doi.org/10.7717/peerj.915
Slabaugh MB, Cooper LD, Kishore VK, Knapp SJ, Kling JG. Genes affecting novel seed constituents in Limnanthes alba Benth: transcriptome analysis of developing embryos and a new genetic map of meadowfoam. PeerJ. 2015 May 19;3:e915. doi: 10.7717/peerj.915. eCollection 2015. PMID: 26038713; PMCID: PMC4451031.
Copy Download .nbib Format: NLM
Share it on

PeerJ. 2015 May 19;3:e915. doi: 10.7717/peerj.915. eCollection 2015.

Genes affecting novel seed constituents in Limnanthes alba Benth: transcriptome analysis of developing embryos and a new genetic map of meadowfoam.

PeerJ

Mary B Slabaugh, Laurel D Cooper, Venkata K Kishore, Steven J Knapp, Jennifer G Kling

Affiliations

  1. Department of Crop and Soil Science, Oregon State University , Corvallis, OR , United States of America.
  2. Department of Crop and Soil Science, Oregon State University , Corvallis, OR , United States of America ; Department of Botany and Plant Pathology, Oregon State University , Corvallis OR , United States of America.
  3. Monsanto Company , Bengaluru Area , India.
  4. Department of Plant Sciences, University of California-Davis , Davis, CA , United States of America.

PMID: 26038713 PMCID: PMC4451031 DOI: 10.7717/peerj.915

Abstract

The seed oil of meadowfoam, a new crop in the Limnanthaceae family, is highly enriched in very long chain fatty acids that are desaturated at the Δ5 position. The unusual oil is desirable for cosmetics and innovative industrial applications and the seed meal remaining after oil extraction contains glucolimnanthin, a methoxylated benzylglucosinolate whose degradation products are herbicidal and anti-microbial. Here we describe EST analysis of the developing seed transcriptome that identified major genes involved in biosynthesis and assembly of the seed oil and in glucosinolate metabolic pathways. mRNAs encoding acyl-CoA Δ5 desaturase were notably abundant. The library was searched for simple sequence repeats (SSRs) and single nucleotide polymorphisms (SNPs). Fifty-four new SSR markers and eight candidate gene markers were developed and combined with previously developed SSRs to construct a new genetic map for Limnanthes alba. Mapped genes in the lipid biosynthetic pathway encode 3-ketoacyl-CoA synthase (KCS), Δ5 desaturase (Δ5DS), lysophosphatidylacyl-acyl transferase (LPAT), and acyl-CoA diacylglycerol acyl transferase (DGAT). Mapped genes in glucosinolate biosynthetic and degradation pathways encode CYP79A, myrosinase (TGG), and epithiospecifier modifier protein (ESM). The resources developed in this study will further the domestication and improvement of meadowfoam as an oilseed crop.

Keywords: Desaturase; Glucolimnanthin; KCS; LPAT; Limnanthes; Meadowfoam

References

  1. Theor Appl Genet. 2009 Feb;118(4):765-73 - PubMed
  2. Plant Physiol. 1992 Aug;99(4):1711-5 - PubMed
  3. Plant Physiol. 1990 Nov;94(3):1199-206 - PubMed
  4. Genome. 2009 Jun;52(6):547-56 - PubMed
  5. PLoS One. 2012;7(11):e49261 - PubMed
  6. Plant Physiol. 1993 Jun;102(2):609-613 - PubMed
  7. Theor Appl Genet. 2004 Feb;108(3):450-7 - PubMed
  8. J Cosmet Sci. 2001 Mar-Apr;52(2):149-50 - PubMed
  9. Arch Biochem Biophys. 1981 Jul;209(2):376-84 - PubMed
  10. J Agric Food Chem. 2009 Mar 11;57(5):1821-6 - PubMed
  11. Plant Physiol. 2000 Sep;124(1):243-51 - PubMed
  12. Bioinformatics. 2003 Feb 12;19(3):421-2 - PubMed
  13. Plant Physiol. 2008 Sep;148(1):89-96 - PubMed
  14. Plant J. 2011 Dec;68(6):1014-27 - PubMed
  15. J Exp Bot. 2008;59(13):3543-9 - PubMed
  16. Eur J Biochem. 2002 Oct;269(19):4789-98 - PubMed
  17. Plant Physiol. 1980 Oct;66(4):649-55 - PubMed
  18. Plant Cell. 2006 Jun;18(6):1524-36 - PubMed
  19. J Biol Chem. 2000 May 12;275(19):14659-66 - PubMed
  20. Plant Physiol. 1995 Dec;109 (4):1389-94 - PubMed
  21. J Agric Food Chem. 2012 Jan 11;60(1):339-45 - PubMed
  22. Plant Physiol. 2004 Sep;136(1):2665-75 - PubMed
  23. Nucleic Acids Res. 1991 Jul 25;19(14):4009 - PubMed
  24. Nature. 2012 Aug 23;488(7412):531-4 - PubMed
  25. Biochem Biophys Res Commun. 2006 Jul 28;346(2):583-90 - PubMed
  26. Plant Physiol. 2007 May;144(1):455-67 - PubMed
  27. J Chem Ecol. 1996 Oct;22(10):1939-49 - PubMed
  28. Plant Physiol. 2004 Dec;136(4):4237-45 - PubMed
  29. Annu Rev Plant Biol. 2007;58:295-319 - PubMed
  30. J Exp Bot. 2011 Jan;62(2):521-32 - PubMed
  31. Biochem J. 2002 Jun 15;364(Pt 3):795-805 - PubMed
  32. J Struct Biol. 2008 Apr;162(1):50-62 - PubMed
  33. Plant Mol Biol. 2008 Jul;67(5):547-66 - PubMed
  34. Plant Cell. 2006 Sep;18(9):2294-313 - PubMed
  35. Nat Genet. 2008 Mar;40(3):367-72 - PubMed
  36. Plant Physiol. 2001 Jun;126(2):861-74 - PubMed

Publication Types