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Shen WX, Au PC, Shi BJ, et al. Satellite RNAs interfere with the function of viral RNA silencing suppressors. Front Plant Sci. 2015;6:281doi: 10.3389/fpls.2015.00281.
Shen, W. X., Au, P. C., Shi, B. J., Smith, N. A., Dennis, E. S., Guo, H. S., Zhou, C. Y., & Wang, M. B. (2015). Satellite RNAs interfere with the function of viral RNA silencing suppressors. Frontiers in plant science, 6281. https://doi.org/10.3389/fpls.2015.00281
Shen, Wan-Xia, et al. "Satellite RNAs interfere with the function of viral RNA silencing suppressors." Frontiers in plant science vol. 6 (2015): 281. doi: https://doi.org/10.3389/fpls.2015.00281
Shen WX, Au PC, Shi BJ, Smith NA, Dennis ES, Guo HS, Zhou CY, Wang MB. Satellite RNAs interfere with the function of viral RNA silencing suppressors. Front Plant Sci. 2015 Apr 24;6:281. doi: 10.3389/fpls.2015.00281. eCollection 2015. PMID: 25964791; PMCID: PMC4408847.
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Front Plant Sci. 2015 Apr 24;6:281. doi: 10.3389/fpls.2015.00281. eCollection 2015.

Satellite RNAs interfere with the function of viral RNA silencing suppressors.

Frontiers in plant science

Wan-Xia Shen, Phil Chi Khang Au, Bu-Jun Shi, Neil A Smith, Elizabeth S Dennis, Hui-Shan Guo, Chang-Yong Zhou, Ming-Bo Wang

Affiliations

  1. National Citrus Engineering Research Center, Citrus Research Institute, Southwest University Chongqing, China ; Commonwealth Scientific and Industrial Research Organisation Plant Industry Canberra, ACT, Australia.
  2. Commonwealth Scientific and Industrial Research Organisation Plant Industry Canberra, ACT, Australia.
  3. Department of Plant Science, Waite Institute, Adelaide University Glen Osmond, SA, Australia.
  4. State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences Beijing, China.
  5. National Citrus Engineering Research Center, Citrus Research Institute, Southwest University Chongqing, China.

PMID: 25964791 PMCID: PMC4408847 DOI: 10.3389/fpls.2015.00281

Abstract

Viral satellite RNAs (satRNAs) are small subviral RNAs and depend on the helper virus for replication and spread. satRNAs can attenuate helper virus-induced symptoms, the mechanism of which remains unclear. Here, we show that two virus-encoded suppressors of RNA silencing (VSRs), Cucumber mosaic virus (CMV) 2b and Tombusvirus P19, suppress hairpin RNA (hpRNA)-induced silencing of a β-glucuronidase (GUS) gene in Nicotiana benthamiana. This suppression can be overcome by CMV Y-satellite RNA (Y-Sat) via the Y-Sat-derived small interfering RNAs (siRNAs), which bind to the VSRs and displace the bound hpGUS-derived siRNAs. We also show that microRNA target gene expression in N. tabacum was elevated by CMV infection, presumably due to function of the 2b VSR, but this upregulation of microRNA target genes was reversed in the presence of Y-Sat. These results suggest that satRNA infection minimizes the effect of VSRs on host siRNA and microRNA-directed silencing. Our results suggest that the high abundance of satRNA-derived siRNAs contributes to symptom attenuation by binding helper virus-encoded VSRs, minimizing the capacity of the VSRs to bind host siRNA and miRNA and interfere with their function.

Keywords: RNA silencing; microRNA expression; satellite RNA; symptom attenuation; virus-encoded suppressor

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