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Igarashi T, Iwasaki N, Kawamura D, et al. Therapeutic Effects of Intra-Articular Ultrapurified Low Endotoxin Alginate Administration on Experimental Osteoarthritis in Rabbits. Cartilage. 2012;3(1):70-8doi: 10.1177/1947603511418959.
Igarashi, T., Iwasaki, N., Kawamura, D., Tsukuda, Y., Kasahara, Y., Todoh, M., Tadano, S., & Minami, A. (2012). Therapeutic Effects of Intra-Articular Ultrapurified Low Endotoxin Alginate Administration on Experimental Osteoarthritis in Rabbits. Cartilage, 3(1), 70-8. https://doi.org/10.1177/1947603511418959
Igarashi, Tatsuya, et al. "Therapeutic Effects of Intra-Articular Ultrapurified Low Endotoxin Alginate Administration on Experimental Osteoarthritis in Rabbits." Cartilage vol. 3,1 (2012): 70-8. doi: https://doi.org/10.1177/1947603511418959
Igarashi T, Iwasaki N, Kawamura D, Tsukuda Y, Kasahara Y, Todoh M, Tadano S, Minami A. Therapeutic Effects of Intra-Articular Ultrapurified Low Endotoxin Alginate Administration on Experimental Osteoarthritis in Rabbits. Cartilage. 2012 Jan;3(1):70-8. doi: 10.1177/1947603511418959. PMID: 26069620; PMCID: PMC4297190.
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Cartilage. 2012 Jan;3(1):70-8. doi: 10.1177/1947603511418959.

Therapeutic Effects of Intra-Articular Ultrapurified Low Endotoxin Alginate Administration on Experimental Osteoarthritis in Rabbits.

Cartilage

Tatsuya Igarashi, Norimasa Iwasaki, Daisuke Kawamura, Yukinori Tsukuda, Yasuhiko Kasahara, Masahiro Todoh, Shigeru Tadano, Akio Minami

Affiliations

  1. Department of Orthopaedic Surgery, Hokkaido University School of Medicine, Sapporo, Japan.
  2. Division of Human Mechanical Systems and Design, Faculty of Engineering, Hokkaido University, Sapporo, Japan.

PMID: 26069620 PMCID: PMC4297190 DOI: 10.1177/1947603511418959

Abstract

OBJECTIVE: We have developed an ultrapurified low endotoxin alginate (UPLE alginate), which can drastically reduce endotoxin levels. Our purposes were to examine the effects of UPLE alginate administration on osteoarthritis (OA) progression and to determine the adequate molecular weight of the UPLE alginate for therapeutic effects.

DESIGN: To induce knee OA, 35 Japanese White rabbits underwent anterior cruciate ligament transection. Intra-articular injections of 0.3 mL solution of each material were started at 4 weeks postoperatively for a total of 5 weekly injections. Seventy knees were divided into the following groups: AL430 (430 kDa molecular weight UPLE alginate), AL1000 (1,000 kDa), AL1700 (1,700 kDa), HA (hyaluronan), and NS (normal saline). At 9 weeks postoperatively, all knees were assessed macroscopically, histologically, and mechanically.

RESULTS: Macroscopically, the UPLE alginate groups exhibited milder cartilage degradation compared to that of the NS and HA groups. Histological findings of the UPLE alginate groups showed an obvious reduction in the severity of OA. The histological scores of Kikuchi et al. were superior in the alginate treatment groups compared to the NS group. The friction coefficient of the AL1000 group was significantly lower than that of the NS and HA groups.

CONCLUSION: This study indicates that our UPLE alginates, especially AL1000, have promising potential as an effective agent in preventing OA progression.

Keywords: alginate; cartilage; intra-articular injection; molecular weight; osteoarthritis

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