Display options
Cite
Cifuentes Castro VH, López Valenzuela CL, Salazar Sánchez JC, et al. An update of the classical and novel methods used for measuring fast neurotransmitters during normal and brain altered function. Curr Neuropharmacol. 2014;12(6):490-508doi: 10.2174/1570159X13666141223223657.
Cifuentes Castro, V. H., López Valenzuela, C. L., Salazar Sánchez, J. C., Peña, K. P., López Pérez, S. J., Ibarra, J. O., & Villagrán, A. M. (2014). An update of the classical and novel methods used for measuring fast neurotransmitters during normal and brain altered function. Current neuropharmacology, 12(6), 490-508. https://doi.org/10.2174/1570159X13666141223223657
Cifuentes Castro, Victor Hugo, et al. "An update of the classical and novel methods used for measuring fast neurotransmitters during normal and brain altered function." Current neuropharmacology vol. 12,6 (2014): 490-508. doi: https://doi.org/10.2174/1570159X13666141223223657
Cifuentes Castro VH, López Valenzuela CL, Salazar Sánchez JC, Peña KP, López Pérez SJ, Ibarra JO, Villagrán AM. An update of the classical and novel methods used for measuring fast neurotransmitters during normal and brain altered function. Curr Neuropharmacol. 2014 Dec;12(6):490-508. doi: 10.2174/1570159X13666141223223657. PMID: 25977677; PMCID: PMC4428024.
Copy Download .nbib Format: NLM
Share it on

Curr Neuropharmacol. 2014 Dec;12(6):490-508. doi: 10.2174/1570159X13666141223223657.

An update of the classical and novel methods used for measuring fast neurotransmitters during normal and brain altered function.

Current neuropharmacology

Victor Hugo Cifuentes Castro, Carmen Lucía López Valenzuela, Juan Carlos Salazar Sánchez, Kenia Pardo Peña, Silvia J López Pérez, Jorge Ortega Ibarra, Alberto Morales Villagrán

Affiliations

  1. Department of Molecular and Cellular Biology, Camino Ramón Padilla Sánchez 2100, Nextipac, Zapopan, Jalisco, México, Zip code: 45110, Mexico.

PMID: 25977677 PMCID: PMC4428024 DOI: 10.2174/1570159X13666141223223657

Abstract

To understand better the cerebral functions, several methods have been developed to study the brain activity, they could be related with morphological, electrophysiological, molecular and neurochemical techniques. Monitoring neurotransmitter concentration is a key role to know better how the brain works during normal or pathological conditions, as well as for studying the changes in neurotransmitter concentration with the use of several drugs that could affect or reestablish the normal brain activity. Immediate response of the brain to environmental conditions is related with the release of the fast acting neurotransmission by glutamate (Glu), γ-aminobutyric acid (GABA) and acetylcholine (ACh) through the opening of ligand-operated ion channels. Neurotransmitter release is mainly determined by the classical microdialysis technique, this is generally coupled to high performance liquid chromatography (HPLC). Detection of neurotransmitters can be done by fluorescence, optical density, electrochemistry or other detection systems more sophisticated. Although the microdialysis method is the golden technique to monitor the brain neurotransmitters, it has a poor temporal resolution. Recently, with the use of biosensor the drawback of temporal resolution has been improved considerably, however other inconveniences have merged, such as stability, reproducibility and the lack of reliable biosensors mainly for GABA. The aim of this review is to show the important advances in the different ways to measure neurotransmitter concentrations; both with the use of classic techniques as well as with the novel methods and alternant approaches to improve the temporal resolution.

Keywords: Acetylcholine; GABA; Glutamate; measurement

References

  1. Curr Protoc Neurosci. 2009 Apr;Chapter 7:Unit7.1 - PubMed
  2. Brain Res. 1994 Oct 3;659(1-2):117-25 - PubMed
  3. J Neurosci Methods. 2007 Jan 15;159(1):86-92 - PubMed
  4. Rapid Commun Mass Spectrom. 2007;21(18):2933-43 - PubMed
  5. Epilepsia. 2000 Jun;41(6):675-80 - PubMed
  6. Brain Res. 2000 Dec 29;887(2):259-65 - PubMed
  7. J Chromatogr B Biomed Sci Appl. 1999 Apr 16;726(1-2):285-90 - PubMed
  8. Annu Rev Pharmacol Toxicol. 1981;21:165-204 - PubMed
  9. Anal Chem. 2003 Nov 1;75(21):5854-9 - PubMed
  10. Anal Chem. 2004 Feb 15;76(4):1098-106 - PubMed
  11. Brain Res Dev Brain Res. 1997 Dec 19;104(1-2):19-30 - PubMed
  12. J Neurosci Methods. 2010 Jun 15;189(2):210-5 - PubMed
  13. Biosens Bioelectron. 2012 Mar 15;33(1):44-9 - PubMed
  14. Biosens Bioelectron. 2014 Feb 15;52:397-402 - PubMed
  15. NMR Biomed. 2004 Apr;17(2):60-8 - PubMed
  16. Am J Alzheimers Dis Other Demen. 2011 Sep;26(6):450-6 - PubMed
  17. Analyst. 2014 Oct 7;139(19):4994-5000 - PubMed
  18. Curr Top Med Chem. 2006;6(10):935-40 - PubMed
  19. Brain Res Bull. 2001 Jul 1;55(4):555-60 - PubMed
  20. J Pharm Biomed Anal. 2008 Aug 5;47(4-5):870-5 - PubMed
  21. J Neurochem. 1985 Nov;45(5):1649-52 - PubMed
  22. Front Psychiatry. 2014 May 19;5:47 - PubMed
  23. Nat Methods. 2010 Feb;7(2):126-9 - PubMed
  24. Anal Chem. 2007 Nov 15;79(22):8747-54 - PubMed
  25. J Neurosci Methods. 2008 Jun 30;171(2):190-6 - PubMed
  26. Sensors (Basel). 2013 Aug 30;13(9):11498-506 - PubMed
  27. J Biolumin Chemilumin. 1994 Mar-Apr;9(2):65-72 - PubMed
  28. Toxicol Appl Pharmacol. 2009 May 1;236(3):341-7 - PubMed
  29. Rapid Commun Mass Spectrom. 2009 Oct 30;23(20):3313-22 - PubMed
  30. Anal Chem. 2008 Nov 15;80(22):8576-82 - PubMed
  31. Arch Toxicol. 2011 Dec;85(12 ):1607-16 - PubMed
  32. Anal Chem. 2002 Feb 1;74(3):539-46 - PubMed
  33. J Neurosci Methods. 2010 Dec 15;194(1):87-93 - PubMed
  34. Peptides. 1993 Nov-Dec;14(6):1125-32 - PubMed
  35. J Chromatogr. 1993 Dec 22;622(2):125-35 - PubMed
  36. J Am Chem Soc. 2012 Nov 21;134(46):19026-34 - PubMed
  37. Analyst. 2004 Dec;129(12):1157-65 - PubMed
  38. Exp Neurol. 2014 Jul;257:39-49 - PubMed
  39. Clin Chem. 1993 Feb;39(2):247-50 - PubMed
  40. Neuropharmacology. 2011 Jul-Aug;61(1-2):172-80 - PubMed
  41. ACS Chem Neurosci. 2012 Apr 18;3(4):285-292 - PubMed
  42. J Neurosci. 2002 Oct 15;22(20):9134-41 - PubMed
  43. J Neurosci Methods. 2002 Nov 15;121(1):33-9 - PubMed
  44. Epilepsy Res. 1996 Dec;26(1):245-54 - PubMed
  45. J Magn Reson Imaging. 2008 Apr;27(4):710-7 - PubMed
  46. Int Rev Neurobiol. 1975;17:1-36 - PubMed
  47. J Neurosci Methods. 2009 Mar 15;177(2):289-93 - PubMed
  48. J Neurosci. 2012 Nov 7;32(45):15779-90 - PubMed
  49. Anal Chem. 2013 Dec 17;85(24):11686-90 - PubMed
  50. Brain. 2002 Aug;125(Pt 8):1908-22 - PubMed
  51. Rapid Commun Mass Spectrom. 2005;19(20):2950-6 - PubMed
  52. Exp Brain Res. 1989;75(3):659-63 - PubMed
  53. Sensors (Basel). 2009;9(6):4034-55 - PubMed
  54. Neuroscience. 1990;37(1):187-91 - PubMed
  55. J Electroanal Chem (Lausanne). 2012 Aug 15;682:141-146 - PubMed
  56. J Chromatogr B Biomed Appl. 1995 Aug 18;670(2):323-7 - PubMed
  57. Anal Chem. 2006 Feb 1;78(3):835-43 - PubMed
  58. Neuropharmacology. 2011 Oct-Nov;61(5-6):891-9 - PubMed
  59. Magn Reson Imaging. 2007 Sep;25(7):1032-8 - PubMed
  60. J Chromatogr B Biomed Sci Appl. 1999 Feb 19;723(1-2):47-59 - PubMed
  61. Anal Chem. 1972 Sep;44(13):1879-81 - PubMed
  62. J Pharm Biomed Anal. 2010 Dec 15;53(5):1180-92 - PubMed
  63. J Physiol. 1936 May 4;86(4):353-80 - PubMed
  64. J Chromatogr B Biomed Sci Appl. 2001 Jun 15;757(2):277-83 - PubMed
  65. Angew Chem Int Ed Engl. 2000 Nov 17;39(22):4004-4032 - PubMed
  66. Pharmacol Ther. 1998 Jan;77(1):59-79 - PubMed
  67. Biosens Bioelectron. 2005 Jul 15;21(1):87-94 - PubMed
  68. Chem Rev. 2008 Jul;108(7):2554-84 - PubMed
  69. J Biol Chem. 1958 May;232(1):403-15 - PubMed
  70. Anal Chem. 2006 Dec 1;78(23):8121-8 - PubMed
  71. J Neurosci Methods. 2004 Dec 30;140(1-2):29-38 - PubMed
  72. Epilepsia. 1999 Jul;40(7):958-64 - PubMed
  73. Nature. 1993 Jan 14;361(6408):186-7 - PubMed
  74. Neuropharmacology. 2013 Jan;64:294-304 - PubMed
  75. Seizure. 2003 Jul;12(5):300-6 - PubMed
  76. Pharmacol Biochem Behav. 2008 Aug;90(2):135-47 - PubMed
  77. J Physiol. 1996 Dec 15;497 ( Pt 3):745-51 - PubMed
  78. Curr Protoc Neurosci. 2009 Jul;Chapter 7:Unit 7.4.1-30 - PubMed
  79. PLoS One. 2014 Mar 13;9(3):e91120 - PubMed
  80. Brain Res Bull. 2000 Oct;53(3):255-62 - PubMed
  81. J Neurochem. 1998 Feb;70(2):617-25 - PubMed
  82. J Agric Food Chem. 2008 Sep 10;56(17):7637-43 - PubMed
  83. PLoS One. 2014 Jun 12;9(6):e98692 - PubMed
  84. Arch Int Pharmacodyn Ther. 1972;198(1):9-21 - PubMed
  85. ACS Chem Neurosci. 2011 Nov 16;2(11):658-666 - PubMed
  86. Life Sci. 1998;63(15):1289-303 - PubMed
  87. J Neurosci Methods. 2007 Mar 15;160(2):223-30 - PubMed
  88. Electrophoresis. 2007 Apr;28(7):1100-6 - PubMed
  89. Anal Bioanal Chem. 2010 Dec;398(7-8):3015-21 - PubMed
  90. Spectrochim Acta A Mol Biomol Spectrosc. 2015 Feb 5;136 Pt C:1962-6 - PubMed
  91. Cell Tissue Res. 2013 Oct;354(1):27-39 - PubMed
  92. Nat Commun. 2012;3:1031 - PubMed
  93. J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Sep 5;808(2):169-75 - PubMed
  94. Nanotechnology. 2013 Jul 19;24(28):285501 - PubMed
  95. J Neurosci Methods. 2010 May 30;189(1):14-22 - PubMed
  96. Neurochem Res. 2012 Jul;37(7):1457-64 - PubMed
  97. Gen Pharmacol. 1998 Apr;30(4):601-4 - PubMed
  98. Neuropsychopharmacology. 2001 Jul;25(1):1-27 - PubMed
  99. J Agric Food Chem. 2009 Jan 14;57(1):170-5 - PubMed
  100. Eur J Neurosci. 2007 Apr;25(8):2249-59 - PubMed
  101. Brain Res Brain Res Protoc. 2005 Feb;14(2):61-6 - PubMed
  102. J Neurochem. 1996 Dec;67(6):2624-7 - PubMed
  103. J Pharm Biomed Anal. 2007 Jun 28;44(2):586-93 - PubMed
  104. Synapse. 1997 Nov;27(3):242-61 - PubMed
  105. Biosens Bioelectron. 2010 Dec 15;26(4):1420-4 - PubMed
  106. Brain Res. 2007 Aug 8;1162:121-9 - PubMed
  107. Talanta. 1998 Aug;46(6):1223-36 - PubMed
  108. Ann Neurol. 1998 Dec;44(6):948-52 - PubMed
  109. Neuroscience. 1992 Nov;51(1):55-64 - PubMed
  110. Rapid Commun Mass Spectrom. 2000;14(18):1695-700 - PubMed
  111. J Neurochem. 1988 Oct;51(4):1308-10 - PubMed
  112. J Agric Food Chem. 2008 Jul 23;56(14):5538-43 - PubMed
  113. Anal Chem. 2011 May 1;83(9):3563-71 - PubMed
  114. Naunyn Schmiedebergs Arch Pharmacol. 1988 Dec;338(6):658-63 - PubMed
  115. Ann Neurol. 2003;54 Suppl 6:S3-12 - PubMed
  116. Epilepsia. 2001 Apr;42(4):543-8 - PubMed
  117. Nature. 1997 Sep 25;389(6649):385-9 - PubMed
  118. Biosens Bioelectron. 2010 Mar 15;25(7):1597-602 - PubMed
  119. J Biol Chem. 1959 Apr;234(4):932-6 - PubMed
  120. Sensors (Basel). 2008;8(8):5023-5036 - PubMed
  121. Biosens Bioelectron. 2005 Jun 15;20(12):2388-403 - PubMed
  122. J Phys Chem B. 2005 Nov 3;109(43):20620-8 - PubMed
  123. Rapid Commun Mass Spectrom. 2007;21(22):3621-8 - PubMed
  124. Neurosci Lett. 1992 Dec 14;148(1-2):19-22 - PubMed
  125. Mol Psychiatry. 2002;7(3):302-10 - PubMed
  126. J Chromatogr B Analyt Technol Biomed Life Sci. 2011 Dec 15;879(32):3927-31 - PubMed
  127. Brain Res. 2006 Nov 6;1118(1):34-42 - PubMed
  128. Chem Res Toxicol. 1989 Sep-Oct;2(5):307-11 - PubMed
  129. J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Apr 1;848(2):323-8 - PubMed
  130. J Neurosci. 2003 Apr 1;23 (7):2627-33 - PubMed
  131. Anal Chim Acta. 2013 Mar 20;768:12-34 - PubMed
  132. J Neurosci Methods. 2008 Feb 15;168(1):48-53 - PubMed
  133. Brain Res. 1984 Jan 9;290(2):390-5 - PubMed
  134. Anal Chem. 2005 Aug 1;77(15):4854-60 - PubMed
  135. Eur J Neurosci. 2001 Jan;13(1):68-78 - PubMed
  136. J Neurosci. 2014 Jan 8;34(2):506-14 - PubMed
  137. Biol Psychiatry. 1999 Oct 1;46(7):997-1002 - PubMed
  138. Curr Opin Chem Biol. 2013 Oct;17(5):860-7 - PubMed
  139. J Magn Reson Imaging. 2011 May;33(5):1262-7 - PubMed
  140. PLoS One. 2014 Feb 13;9(2):e88667 - PubMed
  141. Anal Chim Acta. 2009 Sep 28;651(1):1-14 - PubMed
  142. J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Jul 5;806(2):237-44 - PubMed
  143. Magn Reson Imaging. 1999 Apr;17(3):417-25 - PubMed
  144. Eur J Pharmacol. 1999 Jun 4;373(2-3):153-61 - PubMed
  145. J Neurochem. 2006 Mar;96(6):1626-35 - PubMed
  146. Biol Mass Spectrom. 1991 Jan;20(1):21-5 - PubMed
  147. Artif Cells Blood Substit Immobil Biotechnol. 2010 Aug;38(4):178-85 - PubMed
  148. ACS Nano. 2013 Aug 27;7(8):7278-86 - PubMed
  149. Appl Phys Lett. 2013 Jun 24;102(25):253107 - PubMed
  150. Ther Adv Neurol Disord. 2011 Jan;4(1):15-24 - PubMed
  151. Magn Reson Med. 2002 May;47(5):1009-12 - PubMed
  152. J Microbiol Methods. 2011 Jan;84(1):137-9 - PubMed
  153. Front Comput Neurosci. 2013 May 29;7:66 - PubMed
  154. Neurochem Int. 2013 Dec;63(7):696-701 - PubMed
  155. Naunyn Schmiedebergs Arch Pharmacol. 1989 Jun;339(6):603-7 - PubMed
  156. Neuroscientist. 2009 Apr;15(2):126-33 - PubMed
  157. Anal Chem. 1999 Nov 15;71(22):5093-100 - PubMed
  158. Faraday Discuss. 2014;172:457-72 - PubMed
  159. J Neurochem. 2008 Aug;106(3):1035-43 - PubMed
  160. Sci Rep. 2014 Jan 17;4:3738 - PubMed
  161. J Chromatogr B Biomed Sci Appl. 1998 Apr 24;708(1-2):49-54 - PubMed
  162. Nat Genet. 2001 May;28(1):49-52 - PubMed
  163. Neuropsychopharmacology. 2011 Jul;36(8):1769-77 - PubMed
  164. Biosens Bioelectron. 2014 Feb 15;52:304-9 - PubMed
  165. Proc Natl Acad Sci U S A. 1993 Jun 15;90(12):5662-6 - PubMed
  166. Neuropharmacology. 1993 Aug;32(8):745-50 - PubMed
  167. Eur J Pharmacol. 2007 Jan 26;555(2-3):122-8 - PubMed
  168. Nat Neurosci. 2010 Jan;13(1):127-32 - PubMed
  169. Pharmacol Rep. 2014 Feb;66(1):49-55 - PubMed
  170. Sensors (Basel). 2013 Oct 16;13(10):14029-40 - PubMed
  171. Anal Sci. 2007 Jan;23(1):81-4 - PubMed
  172. Anal Chem. 1991 Dec 15;63(24):2961-5 - PubMed
  173. Neuropsychopharmacology. 1998 May;18(5):403-4 - PubMed
  174. Brain Res. 1997 Jun 13;759(2):221-7 - PubMed
  175. Anal Bioanal Chem. 2008 Oct;392(4):651-62 - PubMed
  176. J Chromatogr B Analyt Technol Biomed Life Sci. 2011 Jan 1;879(1):56-60 - PubMed
  177. Front Cell Neurosci. 2014 May 15;8:133 - PubMed
  178. Neurology. 1996 May;46(5):1459-63 - PubMed
  179. Biosens Bioelectron. 1991;6(8):675-80 - PubMed
  180. J Neurosci Methods. 1995 Mar;57(1):93-9 - PubMed
  181. J Neurosci Methods. 1989 Jul;29(1):73-6 - PubMed
  182. ACS Chem Neurosci. 2013 Feb 20;4(2):321-9 - PubMed
  183. Anal Bioanal Chem. 2007 Nov;389(6):1961-6 - PubMed
  184. Electrophoresis. 2007 Aug;28(17):3115-21 - PubMed
  185. Anal Biochem. 1998 Jun 1;259(2):265-71 - PubMed
  186. Angew Chem Int Ed Engl. 2014 Jan 27;53(5):1302-5 - PubMed
  187. Biosens Bioelectron. 2012 Jan 15;31(1):110-5 - PubMed
  188. J Neurosci Methods. 1999 Aug 15;90(2):129-42 - PubMed
  189. Anal Chem. 2001 Mar 1;73(5):1037-42 - PubMed
  190. Behav Neurosci. 2003 Apr;117(2):222-7 - PubMed
  191. Talanta. 2010 Nov 15;83(1):269-73 - PubMed
  192. Trends Biotechnol. 2005 Aug;23(8):420-8 - PubMed
  193. Neurosci Res. 2010 Aug;67(4):347-53 - PubMed
  194. Biosens Bioelectron. 2014 Nov 15;61:192-9 - PubMed
  195. Anal Chim Acta. 2009 Feb 9;633(2):223-31 - PubMed
  196. Prog Neuropsychopharmacol Biol Psychiatry. 2003 May;27(3):419-23 - PubMed
  197. Faraday Discuss. 2000;(116):291-303; discussion 335-51 - PubMed
  198. Front Aging Neurosci. 2014 May 13;6:87 - PubMed
  199. Epilepsy Res. 2002 May;49(3):203-10 - PubMed
  200. J Neurosci Methods. 2009 Aug 30;182(1):78-84 - PubMed
  201. J Chromatogr B Analyt Technol Biomed Life Sci. 2003 Feb 5;784(2):385-93 - PubMed
  202. Neuropharmacology. 2014 Jul;82:11-8 - PubMed
  203. Enzyme Microb Technol. 2014 Apr 10;57:69-77 - PubMed
  204. Neuroscience. 2000;101(3):547-61 - PubMed
  205. J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Jan 25;814(2):295-302 - PubMed
  206. Brain Res. 2000 Sep 15;877(1):91-4 - PubMed
  207. Front Neural Circuits. 2013 Oct 31;7:173 - PubMed
  208. Sensors (Basel). 2013 Jun 03;13(6):7296-307 - PubMed
  209. J Neurochem. 1996 Dec;67(6):2399-404 - PubMed
  210. Anal Chem. 2009 Jul 15;81(14):5858-64 - PubMed
  211. J Sep Sci. 2012 Feb;35(4):548-55 - PubMed
  212. Mol Psychiatry. 2004 Nov;9(11):984-97, 979 - PubMed
  213. Prog Nucl Magn Reson Spectrosc. 2012 Jan;60:29-41 - PubMed
  214. Epilepsia. 1995 May;36(5):457-64 - PubMed
  215. Ann N Y Acad Sci. 2003 Nov;1003:292-308 - PubMed
  216. Brain Res Brain Res Rev. 1999 Dec;31(1):6-41 - PubMed
  217. J Neurochem. 2005 Aug;94(3):672-9 - PubMed
  218. Front Cell Neurosci. 2013 May 03;7:55 - PubMed
  219. J Neurochem. 2003 Dec;87(5):1204-12 - PubMed

Publication Types