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Benders KE, Boot W, Cokelaere SM, et al. Multipotent Stromal Cells Outperform Chondrocytes on Cartilage-Derived Matrix Scaffolds. Cartilage. 2014;5(4):221-30doi: 10.1177/1947603514535245.
Benders, K. E., Boot, W., Cokelaere, S. M., Van Weeren, P. R., Gawlitta, D., Bergman, H. J., Saris, D. B., Dhert, W. J., & Malda, J. (2014). Multipotent Stromal Cells Outperform Chondrocytes on Cartilage-Derived Matrix Scaffolds. Cartilage, 5(4), 221-30. https://doi.org/10.1177/1947603514535245
Benders, K E M, et al. "Multipotent Stromal Cells Outperform Chondrocytes on Cartilage-Derived Matrix Scaffolds." Cartilage vol. 5,4 (2014): 221-30. doi: https://doi.org/10.1177/1947603514535245
Benders KE, Boot W, Cokelaere SM, Van Weeren PR, Gawlitta D, Bergman HJ, Saris DB, Dhert WJ, Malda J. Multipotent Stromal Cells Outperform Chondrocytes on Cartilage-Derived Matrix Scaffolds. Cartilage. 2014 Oct;5(4):221-30. doi: 10.1177/1947603514535245. PMID: 26069701; PMCID: PMC4335771.
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Cartilage. 2014 Oct;5(4):221-30. doi: 10.1177/1947603514535245.

Multipotent Stromal Cells Outperform Chondrocytes on Cartilage-Derived Matrix Scaffolds.

Cartilage

K E M Benders, W Boot, S M Cokelaere, P R Van Weeren, D Gawlitta, H J Bergman, D B F Saris, W J A Dhert, J Malda

Affiliations

  1. Department of Orthopedics, University Medical Center Utrecht, Utrecht, the Netherlands.
  2. Department of Equine Sciences, Utrecht University, Utrecht, the Netherlands.
  3. Lingehoeve, Veterinary Medicine, Lienden, the Netherlands.
  4. Department of Orthopedics, University Medical Center Utrecht, Utrecht, the Netherlands ; MIRA Institute, Department of Tissue Regeneration, University of Twente, Enschede, the Netherlands.
  5. Department of Orthopedics, University Medical Center Utrecht, Utrecht, the Netherlands ; Faculty of Veterinary Sciences, Utrecht University, Utrecht, the Netherlands.
  6. Department of Orthopedics, University Medical Center Utrecht, Utrecht, the Netherlands ; Department of Equine Sciences, Utrecht University, Utrecht, the Netherlands.

PMID: 26069701 PMCID: PMC4335771 DOI: 10.1177/1947603514535245

Abstract

OBJECTIVE: Although extracellular matrix (ECM)-derived scaffolds have been extensively studied and applied in a number of clinical applications, the use of ECM as a biomaterial for (osteo)chondral regeneration is less extensively explored. This study aimed at evaluating the chondrogenic potential of cells seeded on cartilage-derived matrix (CDM) scaffolds in vitro.

DESIGN: Scaffolds were generated from decellularized equine articular cartilage and seeded with either chondrocytes or multipotent stromal cells (MSCs). After 2, 4, and 6 weeks of in vitro culture, CDM constructs were analyzed both histologically (hematoxylin and eosin, Safranin-O, collagen types I and II) and biochemically (glycosaminoglycan [GAG] and DNA content).

RESULTS: After 4 weeks, both cell types demonstrated chondrogenic differentiation; however, the MSCs significantly outperformed chondrocytes in producing new GAG-containing cartilaginous matrix.

CONCLUSION: These promising in vitro results underscore the potency of CDM scaffolds in (osteo)chondral defect repair.

Keywords: cartilage tissue engineering; chondrocyte; extracellular matrix (ECM); mesenchymal stromal cell; scaffold

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