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Olivo RA, da Silva MV, Garcia FB, et al. Evaluation of the effectiveness of packed red blood cell irradiation by a linear accelerator. Rev Bras Hematol Hemoter. 2015;37(3):153-9doi: 10.1016/j.bjhh.2015.03.001.
Olivo, R. A., da Silva, M. V., Garcia, F. B., Soares, S., Rodrigues Junior, V., & Moraes-Souza, H. (2015). Evaluation of the effectiveness of packed red blood cell irradiation by a linear accelerator. Revista brasileira de hematologia e hemoterapia, 37(3), 153-9. https://doi.org/10.1016/j.bjhh.2015.03.001
Olivo, Ricardo Aparecido, et al. "Evaluation of the effectiveness of packed red blood cell irradiation by a linear accelerator." Revista brasileira de hematologia e hemoterapia vol. 37,3 (2015): 153-9. doi: https://doi.org/10.1016/j.bjhh.2015.03.001
Olivo RA, da Silva MV, Garcia FB, Soares S, Rodrigues Junior V, Moraes-Souza H. Evaluation of the effectiveness of packed red blood cell irradiation by a linear accelerator. Rev Bras Hematol Hemoter. 2015 May-Jun;37(3):153-9. doi: 10.1016/j.bjhh.2015.03.001. Epub 2015 Apr 23. PMID: 26041416; PMCID: PMC4459437.
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Rev Bras Hematol Hemoter. 2015 May-Jun;37(3):153-9. doi: 10.1016/j.bjhh.2015.03.001. Epub 2015 Apr 23.

Evaluation of the effectiveness of packed red blood cell irradiation by a linear accelerator.

Revista brasileira de hematologia e hemoterapia

Ricardo Aparecido Olivo, Marcus Vinícius da Silva, Fernanda Bernadelli Garcia, Sheila Soares, Virmondes Rodrigues Junior, Helio Moraes-Souza

Affiliations

  1. Universidade Federal do Triângulo Mineiro (UFTM), Uberaba, MG, Brazil. Electronic address: [email protected].
  2. Universidade Federal do Triângulo Mineiro (UFTM), Uberaba, MG, Brazil.

PMID: 26041416 PMCID: PMC4459437 DOI: 10.1016/j.bjhh.2015.03.001

Abstract

Irradiation of blood components with ionizing radiation generated by a specific device is recommended to prevent transfusion-associated graft-versus-host disease. However, a linear accelerator can also be used in the absence of such a device, which is the case of the blood bank facility studied herein. In order to evaluate the quality of the irradiated packed red blood cells, this study aimed to determine whether the procedure currently employed in the facility is effective in inhibiting the proliferation of T lymphocytes without damaging blood components. The proliferation of T lymphocytes, plasma potassium levels, and the degree of hemolysis were evaluated and compared to blood bags that received no irradiation. Packed red blood cell bags were irradiated at a dose of 25Gy in a linear accelerator. For this purpose, a container was designed to hold the bags and to ensure even distribution of irradiation as evaluated by computed tomography and dose-volume histogram. Irradiation was observed to inhibit the proliferation of lymphocytes. The percentage of hemolysis in irradiated bags was slightly higher than in non-irradiated bags (p-value >0.05), but it was always less than 0.4% of the red cell mass. Although potassium increased in both groups, it was more pronounced in irradiated red blood cells, especially after seven days of storage, with a linear increase over storage time. The findings showed that, at an appropriate dosage and under validated conditions, the irradiation of packed red blood cells in a linear accelerator is effective, inhibiting lymphocyte proliferation but without compromising the viability of the red cells.

Copyright © 2015 Associação Brasileira de Hematologia, Hemoterapia e Terapia Celular. Published by Elsevier Editora Ltda. All rights reserved.

Keywords: Blood safety; Hemotherapy service; T-lymphocytes

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