Display options
Cite
Lappa IK, Kizis D, Natskoulis PI, et al. Comparative study of growth responses and screening of inter-specific OTA production kinetics by A. carbonarius isolated from grapes. Front Microbiol. 2015;6:502doi: 10.3389/fmicb.2015.00502.
Lappa, I. K., Kizis, D., Natskoulis, P. I., & Panagou, E. Z. (2015). Comparative study of growth responses and screening of inter-specific OTA production kinetics by A. carbonarius isolated from grapes. Frontiers in microbiology, 6502. https://doi.org/10.3389/fmicb.2015.00502
Lappa, Iliada K, et al. "Comparative study of growth responses and screening of inter-specific OTA production kinetics by A. carbonarius isolated from grapes." Frontiers in microbiology vol. 6 (2015): 502. doi: https://doi.org/10.3389/fmicb.2015.00502
Lappa IK, Kizis D, Natskoulis PI, Panagou EZ. Comparative study of growth responses and screening of inter-specific OTA production kinetics by A. carbonarius isolated from grapes. Front Microbiol. 2015 May 27;6:502. doi: 10.3389/fmicb.2015.00502. eCollection 2015. PMID: 26074896; PMCID: PMC4444842.
Copy Download .nbib Format: NLM
Share it on

Front Microbiol. 2015 May 27;6:502. doi: 10.3389/fmicb.2015.00502. eCollection 2015.

Comparative study of growth responses and screening of inter-specific OTA production kinetics by A. carbonarius isolated from grapes.

Frontiers in microbiology

Iliada K Lappa, Dimosthenis Kizis, Pantelis I Natskoulis, Efstathios Z Panagou

Affiliations

  1. Laboratory of Microbiology and Biotechnology of Foods, Department of Food Science and Human Nutrition, Agricultural University of Athens Athens, Greece.
  2. Laboratory of Microbiology and Biotechnology of Foods, Department of Food Science and Human Nutrition, Agricultural University of Athens Athens, Greece ; Laboratory of Mycology, Department of Phytopathology, Benaki Phytopathological Institute Athens, Greece.

PMID: 26074896 PMCID: PMC4444842 DOI: 10.3389/fmicb.2015.00502

Abstract

The aim of this work was to assess OchratoxinA (OTA) production of different Aspergillus carbonarius isolates, evaluate their growth profile through different growth measurements, and reveal any underlying correlation between them. Ten different isolates of A. carbonarius isolated from Greek vineyards located in different geographical regions were examined in vitro for their OTA production potential after an incubation period of up to 11 days. All fungal isolates grew on a synthetic grape juice medium (SGM) similar to grape composition at optimum conditions of temperature and water activity (25°C and 0.98 aw). Samples for OTA determination were removed at 3, 5, 7, 9, and 11 days of growth and analyzed by HPLC. Based on OTA measurements the isolates were characterized by diverse OTA production ranging from 50 to 2000 ppb at day 11. The different fungal growth responses (colony diameter, colony area, biomass, biomass dry weight, and colony density) have been measured and correlated with toxin production by means of principal components analysis (PCA), confirming satisfactory correlation and explained over 99% of data variability. Leudeking-Piret model was also used to study OTA production with time, revealing a mixed-growth associated trend and pointing a fail-safe model with slightly better prediction through colony area. This approach contributes to the assessment of correlation between mycotoxin production and different methods of fungal growth determination in relation to time.

Keywords: Aspergillus carbonarius; OTA; growth assessment; modeling kinetics; predictive mycology

References

  1. J Food Prot. 2001 Jun;64(6):903-6 - PubMed
  2. Food Addit Contam. 2001 Jul;18(7):647-54 - PubMed
  3. Int J Food Microbiol. 2001 Dec 30;71(2-3):139-44 - PubMed
  4. Int J Food Microbiol. 2002 Dec 15;79(3):213-5 - PubMed
  5. Food Addit Contam. 2003 Jan;20(1):74-83 - PubMed
  6. Int J Food Microbiol. 2005 Feb 1;98(2):125-30 - PubMed
  7. Int J Food Microbiol. 2005 Apr 1;99(3):329-41 - PubMed
  8. Appl Environ Microbiol. 1990 Jun;56(6):1875-81 - PubMed
  9. Adv Exp Med Biol. 2006;571:49-67 - PubMed
  10. Int J Food Microbiol. 2006 Sep 1;111 Suppl 1:S61-6 - PubMed
  11. Int J Food Microbiol. 2006 Sep 1;111 Suppl 1:S2-4 - PubMed
  12. Int J Food Microbiol. 2006 Jul 15;110(2):160-4 - PubMed
  13. Food Microbiol. 2006 Dec;23(8):753-6 - PubMed
  14. Food Addit Contam. 2007 Nov;24(11):1275-82 - PubMed
  15. Int J Food Microbiol. 2008 Jan 31;121(2):139-49 - PubMed
  16. Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2008 Feb;25(2):193-202 - PubMed
  17. J Appl Microbiol. 2009 Jul;107(1):257-68 - PubMed
  18. Food Microbiol. 2010 Oct;27(7):909-17 - PubMed
  19. Food Microbiol. 2011 Jun;28(4):727-35 - PubMed
  20. Int J Food Microbiol. 2013 Mar 15;162(2):182-9 - PubMed
  21. Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2013;30(7):1339-47 - PubMed
  22. Food Microbiol. 2013 Dec;36(2):182-90 - PubMed
  23. PLoS One. 2014 Apr 07;9(4):e93923 - PubMed
  24. J Chromatogr B Biomed Appl. 1995 Apr 7;666(1):85-99 - PubMed
  25. Int J Food Microbiol. 1994 Nov;23(3-4):419-31 - PubMed
  26. Appl Environ Microbiol. 1994 Jul;60(7):2650-2 - PubMed
  27. Int J Cancer. 1998 Jul 3;77(1):70-5 - PubMed
  28. Appl Environ Microbiol. 1999 Jan;65(1):138-42 - PubMed

Publication Types