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Fukasawa Y, Ohno N, Saitoh Y, et al. Immunohistochemical and morphofunctional studies of skeletal muscle tissues with electric nerve stimulation by in vivo cryotechnique. Acta Histochem Cytochem. 2015;48(2):27-36doi: 10.1267/ahc.14068.
Fukasawa, Y., Ohno, N., Saitoh, Y., Saigusa, T., Arita, J., & Ohno, S. (2015). Immunohistochemical and morphofunctional studies of skeletal muscle tissues with electric nerve stimulation by in vivo cryotechnique. Acta histochemica et cytochemica, 48(2), 27-36. https://doi.org/10.1267/ahc.14068
Fukasawa, Yuki, et al. "Immunohistochemical and morphofunctional studies of skeletal muscle tissues with electric nerve stimulation by in vivo cryotechnique." Acta histochemica et cytochemica vol. 48,2 (2015): 27-36. doi: https://doi.org/10.1267/ahc.14068
Fukasawa Y, Ohno N, Saitoh Y, Saigusa T, Arita J, Ohno S. Immunohistochemical and morphofunctional studies of skeletal muscle tissues with electric nerve stimulation by in vivo cryotechnique. Acta Histochem Cytochem. 2015 Apr 28;48(2):27-36. doi: 10.1267/ahc.14068. Epub 2015 Apr 16. PMID: 26019372; PMCID: PMC4427562.
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Acta Histochem Cytochem. 2015 Apr 28;48(2):27-36. doi: 10.1267/ahc.14068. Epub 2015 Apr 16.

Immunohistochemical and morphofunctional studies of skeletal muscle tissues with electric nerve stimulation by in vivo cryotechnique.

Acta histochemica et cytochemica

Yuki Fukasawa, Nobuhiko Ohno, Yurika Saitoh, Takeshi Saigusa, Jun Arita, Shinichi Ohno

Affiliations

  1. Department of Anatomy and Molecular Histology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi.
  2. First Department of Physiology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi , 1110 Shimokato, Chuo-city, Yamanashi 409-3898, Japan.

PMID: 26019372 PMCID: PMC4427562 DOI: 10.1267/ahc.14068

Abstract

In this study, morphological and immunohistochemical alterations of skeletal muscle tissues during persistent contraction were examined by in vivo cryotechnique (IVCT). Contraction of gastrocnemius muscles was induced by sciatic nerve stimulation. The IVCT was performed immediately, 3 min or 10 min after the stimulation start. Prominent ripples of muscle fibers or wavy deformation of sarcolemma were detected immediately after the stimulation, but they gradually diminished to normal levels during the stimulation. The relative ratio of sarcomere and A band lengths was the highest in the control group, but it immediately decreased to the lowest level and then gradually recovered at 3 min or 10 min. Although histochemical intensity of PAS reaction was almost homogeneous in muscle tissues of the control group or immediately after the stimulation, it decreased at 3 min or 10 min. Serum albumin was immunolocalized as dot-like patterns within some muscle fibers at 3 min stimulation. These patterns became more prominent at 10 min, and the dots got larger and saccular in some sarcoplasmic regions. However, IgG1 and IgM were immunolocalized in blood vessels under nerve stimulation conditions. Therefore, IVCT was useful to capture the morphofunctional and metabolic changes of heterogeneous muscle fibers during the persistent contraction.

Keywords: cryotechnique; glycogen; muscle contraction; nerve stimulation; serum proteins

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