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Tsoktouridis G, Tsiamis G, Koutinas N, et al. Molecular detection of bacteria in plant tissues, using universal 16S ribosomal DNA degenerated primers. Biotechnol Biotechnol Equip. 2014;28(4):583-591doi: 10.1080/13102818.2014.937139.
Tsoktouridis, G., Tsiamis, G., Koutinas, N., & Mantell, S. (2014). Molecular detection of bacteria in plant tissues, using universal 16S ribosomal DNA degenerated primers. Biotechnology, biotechnological equipment, 28(4), 583-591. https://doi.org/10.1080/13102818.2014.937139
Tsoktouridis, Georgios, et al. "Molecular detection of bacteria in plant tissues, using universal 16S ribosomal DNA degenerated primers." Biotechnology, biotechnological equipment vol. 28,4 (2014): 583-591. doi: https://doi.org/10.1080/13102818.2014.937139
Tsoktouridis G, Tsiamis G, Koutinas N, Mantell S. Molecular detection of bacteria in plant tissues, using universal 16S ribosomal DNA degenerated primers. Biotechnol Biotechnol Equip. 2014 Jul 04;28(4):583-591. doi: 10.1080/13102818.2014.937139. Epub 2014 Oct 22. PMID: 26019546; PMCID: PMC4434121.
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Biotechnol Biotechnol Equip. 2014 Jul 04;28(4):583-591. doi: 10.1080/13102818.2014.937139. Epub 2014 Oct 22.

Molecular detection of bacteria in plant tissues, using universal 16S ribosomal DNA degenerated primers.

Biotechnology, biotechnological equipment

Georgios Tsoktouridis, George Tsiamis, Nikolaos Koutinas, Sinclair Mantell

Affiliations

  1. Department of Agricultural Sciences, Imperial College at Wye, University of London , Ashford , Kent , UK ; Department of Crop Production, School of Agriculture, Alexander Technological Educational Institute of Thessaloniki , Thessaloniki , Greece.
  2. Department of Agricultural Sciences, Imperial College at Wye, University of London , Ashford , Kent , UK ; Department of Environmental and Natural Resources Management, University of Western Greece , Agrinio , Greece.
  3. Department of Crop Production, School of Agriculture, Alexander Technological Educational Institute of Thessaloniki , Thessaloniki , Greece.
  4. Department of Agricultural Sciences, Imperial College at Wye, University of London , Ashford , Kent , UK ; Nakhlatec International Development Advisors , Gödelöv , Genarp , Sweden.

PMID: 26019546 PMCID: PMC4434121 DOI: 10.1080/13102818.2014.937139

Abstract

Highly specific, sensitive and rapid tests are required for the detection and identification of covert bacterial contaminations in plant tissue cultures. Current methods available for this purpose are tedious, time consuming, highly error prone, expensive, require advanced technical expertise and are sometimes ineffective. We report here the development of a sensitive polymerase chain reaction (PCR) based method for the rapid detection and identification of bacteria occurring in plant tissue cultures. A total of 121 16S ribosomal DNA (rDNA) coding regions from 14 different groups of bacteria, algae and plants, available in the

Keywords: 16S rDNA; PCR; bacteria; degenerated primers; diagnostics; plant tissues

References

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