Display options
Cite
Tian Y, Almaraz RT, Choi CH, et al. Identification of sialylated glycoproteins from metabolically oligosaccharide engineered pancreatic cells. Clin Proteomics. 2015;12(1):11doi: 10.1186/s12014-015-9083-8.
Tian, Y., Almaraz, R. T., Choi, C. H., Li, Q. K., Saeui, C., Li, D., Shah, P., Bhattacharya, R., Yarema, K. J., & Zhang, H. (2015). Identification of sialylated glycoproteins from metabolically oligosaccharide engineered pancreatic cells. Clinical proteomics, 12(1), 11. https://doi.org/10.1186/s12014-015-9083-8
Tian, Yuan, et al. "Identification of sialylated glycoproteins from metabolically oligosaccharide engineered pancreatic cells." Clinical proteomics vol. 12,1 (2015): 11. doi: https://doi.org/10.1186/s12014-015-9083-8
Tian Y, Almaraz RT, Choi CH, Li QK, Saeui C, Li D, Shah P, Bhattacharya R, Yarema KJ, Zhang H. Identification of sialylated glycoproteins from metabolically oligosaccharide engineered pancreatic cells. Clin Proteomics. 2015 Apr 11;12(1):11. doi: 10.1186/s12014-015-9083-8. eCollection 2015. PMID: 25987888; PMCID: PMC4434541.
Copy Download .nbib Format: NLM
Share it on

Clin Proteomics. 2015 Apr 11;12(1):11. doi: 10.1186/s12014-015-9083-8. eCollection 2015.

Identification of sialylated glycoproteins from metabolically oligosaccharide engineered pancreatic cells.

Clinical proteomics

Yuan Tian, Ruben T Almaraz, Caitlin H Choi, Qing Kay Li, Christopher Saeui, Danni Li, Punit Shah, Rahul Bhattacharya, Kevin J Yarema, Hui Zhang

Affiliations

  1. Department of Pathology, Johns Hopkins University, 400 N. Broadway, Room 4011, Baltimore, MD 21287 USA.
  2. Department of Biomedical Engineering and the Translational Tissue Engineering Center, Johns Hopkins University School of Medicine, Baltimore, MD USA.

PMID: 25987888 PMCID: PMC4434541 DOI: 10.1186/s12014-015-9083-8

Abstract

In this study, we investigated the use of metabolic oligosaccharide engineering and bio-orthogonal ligation reactions combined with lectin microarray and mass spectrometry to analyze sialoglycoproteins in the SW1990 human pancreatic cancer line. Specifically, cells were treated with the azido N-acetylmannosamine analog, 1,3,4-Bu3ManNAz, to label sialoglycoproteins with azide-modified sialic acids. The metabolically labeled sialoglyproteins were then biotinylated via the Staudinger ligation, and sialoglycopeptides containing azido-sialic acid glycans were immobilized to a solid support. The peptides linked to metabolically labeled sialylated glycans were then released from sialoglycopeptides and analyzed by mass spectrometry; in parallel, the glycans from azido-sialoglycoproteins were characterized by lectin microarrays. This method identified 75 unique N-glycosite-containing peptides from 55 different metabolically labeled sialoglycoproteins of which 42 were previously linked to cancer in the literature. A comparison of two of these glycoproteins, LAMP1 and ORP150, in histological tumor samples showed overexpression of these proteins in the cancerous tissue demonstrating that our approach constitutes a viable strategy to identify and discover sialoglycoproteins associated with cancer, which can serve as biomarkers for cancer diagnosis or targets for therapy.

Keywords: Metabolic oligosaccharide engineering; Pancreatic cancer cells; Sialylated glycoproteins

References

  1. Annu Rev Immunol. 1984;2:103-26 - PubMed
  2. Cancer Metastasis Rev. 2012 Dec;31(3-4):501-18 - PubMed
  3. Mol Cell Proteomics. 2007 Nov;6(11):1933-41 - PubMed
  4. BJU Int. 2002 Sep;90(4):462-6 - PubMed
  5. Dis Markers. 2008;25(1):1-16 - PubMed
  6. Exp Biol Med (Maywood). 2010 Feb;235(2):263-70 - PubMed
  7. Anal Chem. 2011 Nov 15;83(22):8509-16 - PubMed
  8. J Med Chem. 2005 Feb 10;48(3):875-83 - PubMed
  9. J Biol Chem. 2005 Feb 11;280(6):4228-37 - PubMed
  10. J Biol Chem. 1992 Aug 25;267(24):16934-8 - PubMed
  11. Science. 1999 May 21;284(5418):1372-6 - PubMed
  12. Anal Chem. 2011 Jan 1;83(1):240-5 - PubMed
  13. FASEB J. 2011 Aug;25(8):2528-37 - PubMed
  14. Clin Immunol Immunopathol. 1993 Apr;67(1):31-9 - PubMed
  15. Med Sci Monit. 2007 Nov;13(11):BR231-239 - PubMed
  16. Gastroenterology. 2005 May;128(6):1626-41 - PubMed
  17. Molecules. 2012 Mar 26;17(4):3672-89 - PubMed
  18. Appl Biochem Biotechnol. 2012 Aug;167(8):2257-68 - PubMed
  19. Glycobiology. 2009 Dec;19(12):1382-401 - PubMed
  20. Nat Biotechnol. 2009 Apr;27(4):378-86 - PubMed
  21. Lab Invest. 1998 Jun;78(6):699-706 - PubMed
  22. Glycoconj J. 2000 Jul-Sep;17(7-9):485-99 - PubMed
  23. Anal Chem. 2013 Apr 2;85(7):3606-13 - PubMed
  24. Mol Cell Proteomics. 2009 Nov;8(11):2555-69 - PubMed
  25. Cancer Res. 2009 Jan 1;69(1):300-9 - PubMed
  26. J Am Chem Soc. 2007 Jun 13;129(23):7266-7 - PubMed
  27. J Proteome Res. 2007 May;6(5):1864-74 - PubMed
  28. CA Cancer J Clin. 2009 Jul-Aug;59(4):225-49 - PubMed
  29. J Proteome Res. 2007 Nov;6(11):4304-12 - PubMed
  30. Biochimie. 2001 Jul;83(7):623-34 - PubMed
  31. Biochim Biophys Acta. 2007 Feb;1770(2):297-306 - PubMed
  32. Mol Cell Proteomics. 2012 Jun;11(6):M111.011403 - PubMed
  33. Thromb Res. 2012 Jun;129(6):779-86 - PubMed
  34. Mol Pharmacol. 2007 Mar;71(3):860-70 - PubMed
  35. J Biol Chem. 2004 Apr 30;279(18):18342-52 - PubMed
  36. Curr Opin Chem Biol. 1998 Feb;2(1):49-61 - PubMed
  37. J Proteome Res. 2012 Dec 7;11(12):6175-86 - PubMed
  38. Biotechnol Bioeng. 2012 Apr;109(4):992-1006 - PubMed
  39. Cancer Epidemiol Biomarkers Prev. 2007 Oct;16(10):1915-7 - PubMed
  40. Ann Biomed Eng. 2012 Apr;40(4):806-15 - PubMed
  41. J Chromatogr A. 2012 Oct 12;1259:200-12 - PubMed
  42. J Med Chem. 2010 May 27;53(10):4277-84 - PubMed
  43. Science. 2000 Mar 17;287(5460):2007-10 - PubMed
  44. Anal Biochem. 2014 Feb 1;446:76-81 - PubMed
  45. Curr Opin Chem Biol. 2007 Feb;11(1):52-8 - PubMed
  46. J Proteome Res. 2009 Feb;8(2):613-9 - PubMed
  47. Bioinformatics. 2005 Sep 15;21(18):3674-6 - PubMed
  48. Cancer. 1995 Nov 1;76(9):1671-7 - PubMed
  49. Nature. 2007 Apr 26;446(7139):1023-9 - PubMed
  50. J Proteome Res. 2006 Jul;5(7):1792-802 - PubMed
  51. Chem Soc Rev. 2013 May 21;42(10):4443-58 - PubMed
  52. Pathol Int. 2010 Nov;60(11):735-43 - PubMed
  53. Cancer Res. 1989 Nov 1;49(21):6077-84 - PubMed
  54. Curr Opin Chem Biol. 2013 Feb;17(1):41-8 - PubMed
  55. Nat Protoc. 2007;2(2):334-9 - PubMed
  56. Electrophoresis. 2011 May;32(10):1118-28 - PubMed
  57. Mol Cell Proteomics. 2013 Sep;12(9):2568-86 - PubMed
  58. Mol Cell Proteomics. 2010 Nov;9(11):2354-68 - PubMed
  59. Methods Enzymol. 2006;415:230-50 - PubMed
  60. Bioorg Med Chem Lett. 2012 Nov 15;22(22):6929-33 - PubMed
  61. Int J Cancer. 2006 Aug 1;119(3):484-92 - PubMed
  62. Anticancer Agents Med Chem. 2013 Feb;13(2):276-80 - PubMed
  63. Bioorg Med Chem Lett. 2015 Mar 15;25(6):1223-7 - PubMed
  64. Anal Chem. 2011 Sep 15;83(18):7013-9 - PubMed
  65. J Biol Chem. 1999 Mar 5;274(10):6397-404 - PubMed
  66. Mol Cell Proteomics. 2012 Jul;11(7):M112.017558 - PubMed
  67. Glycobiology. 2011 Aug;21(8):1060-70 - PubMed
  68. Cancer Res. 1996 May 15;56(10):2237-44 - PubMed
  69. Nat Biotechnol. 2003 Jun;21(6):660-6 - PubMed
  70. Mol Cell Proteomics. 2011 Jun;10(6):M110.007294 - PubMed
  71. Science. 1997 May 16;276(5315):1125-8 - PubMed
  72. Proteomics Clin Appl. 2010 Feb;4(2):124-32 - PubMed
  73. J Proteome Res. 2011 Mar 4;10(3):1425-31 - PubMed
  74. Methods Mol Biol. 2006;328:177-85 - PubMed
  75. Mol Cell Proteomics. 2007 Oct;6(10):1778-87 - PubMed
  76. J Biol Chem. 1990 Jul 15;265(20):12036-43 - PubMed

Publication Types

Grant support