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Okawa S, Unuma K, Yamada A, et al. Lipopolysaccharide induces expression of collagen VI in the rat lung. J Toxicol Pathol. 2014;28(1):37-41doi: 10.1293/tox.2014-0053.
Okawa, S., Unuma, K., Yamada, A., Aki, T., & Uemura, K. (2015). Lipopolysaccharide induces expression of collagen VI in the rat lung. Journal of toxicologic pathology, 28(1), 37-41. https://doi.org/10.1293/tox.2014-0053
Okawa, Sayuri, et al. "Lipopolysaccharide induces expression of collagen VI in the rat lung." Journal of toxicologic pathology vol. 28,1 (2015): 37-41. doi: https://doi.org/10.1293/tox.2014-0053
Okawa S, Unuma K, Yamada A, Aki T, Uemura K. Lipopolysaccharide induces expression of collagen VI in the rat lung. J Toxicol Pathol. 2015 Jan;28(1):37-41. doi: 10.1293/tox.2014-0053. Epub 2014 Dec 27. PMID: 26023260; PMCID: PMC4337498.
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J Toxicol Pathol. 2015 Jan;28(1):37-41. doi: 10.1293/tox.2014-0053. Epub 2014 Dec 27.

Lipopolysaccharide induces expression of collagen VI in the rat lung.

Journal of toxicologic pathology

Sayuri Okawa, Kana Unuma, Atsushi Yamada, Toshihiko Aki, Koichi Uemura

Affiliations

  1. Department of Forensic Medicine, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8519, Japan.

PMID: 26023260 PMCID: PMC4337498 DOI: 10.1293/tox.2014-0053

Abstract

The involvement of the lung during the septic systemic inflammatory response elicited by administration of lipopolysaccharide (LPS) was investigated. Eight-week-old male Sprague-Dawley rats were injected i.p. with 15 mg/kg LPS. After 24 h, the lungs were excised to evaluate the cellular responses to LPS. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF) analysis revealed that type VI collagen (ColVI) was extremely upregulated during sepsis in the rat lung within the first 24 h of LPS administration. Upregulation of ColVI protein and its mRNA was demonstrated by Western blot analysis, real time PCR, and immunohistochemistry. To the best of our knowledge, this is the first report demonstrating the activation of ColVI in the rat lung at the early stage of systemic inflammation. Activation of ColVI might be involved in sepsis-mediated lung fibrosis at an early stage.

Keywords: LPS; lung; rat; type VI collagen

References

  1. Chest. 1995 Jan;107(1):196-200 - PubMed
  2. J Cell Biol. 1988 Nov;107(5):1995-2006 - PubMed
  3. Am J Pathol. 1994 Jul;145(1):114-25 - PubMed
  4. Electrophoresis. 2003 Jan;24(1-2):276-85 - PubMed
  5. Exp Lung Res. 2012 Feb;38(1):28-36 - PubMed
  6. Adv Drug Deliv Rev. 2003 Nov 28;55(12):1531-46 - PubMed
  7. J Immunol. 2007 Nov 15;179(10 ):7079-86 - PubMed
  8. J Immunol. 2008 Apr 15;180(8):5707-19 - PubMed
  9. Proc Natl Acad Sci U S A. 2008 Sep 2;105(35):12849-54 - PubMed
  10. Biochemistry. 1990 Feb 6;29(5):1245-54 - PubMed
  11. J Cell Biol. 1986 Mar;102(3):703-10 - PubMed
  12. PLoS One. 2013;8(2):e56526 - PubMed
  13. Trends Mol Med. 2013 Jul;19(7):410-7 - PubMed
  14. Am J Respir Crit Care Med. 1995 Jun;151(6):1956-64 - PubMed
  15. Hepatol Res. 2013 Jan;43(1):91-6 - PubMed
  16. Cells Tissues Organs. 2011;194(1):25-37 - PubMed
  17. Am J Physiol Heart Circ Physiol. 2006 Jan;290(1):H323-30 - PubMed
  18. Eur J Biochem. 1983 Jun 1;133(1):39-46 - PubMed

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