Display options
Cite
Erfle DJ, Santerre JP, Labow RS. Lysosomal enzyme release from human neutrophils adherent to foreign material surfaces: enhanced release of elastase activity. Cardiovasc Pathol. 1997;6(6):333-40doi: 10.1016/S1054-8807(97)00031-8.
Erfle, D. J., Santerre, J. P., & Labow, R. S. (1997). Lysosomal enzyme release from human neutrophils adherent to foreign material surfaces: enhanced release of elastase activity. Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology, 6(6), 333-40. https://doi.org/10.1016/S1054-8807(97)00031-8
Erfle, D J, et al. "Lysosomal enzyme release from human neutrophils adherent to foreign material surfaces: enhanced release of elastase activity." Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology vol. 6,6 (1997): 333-40. doi: https://doi.org/10.1016/S1054-8807(97)00031-8
Erfle DJ, Santerre JP, Labow RS. Lysosomal enzyme release from human neutrophils adherent to foreign material surfaces: enhanced release of elastase activity. Cardiovasc Pathol. 1997 Nov-Dec;6(6):333-40. doi: 10.1016/S1054-8807(97)00031-8. PMID: 25989886.
Copy Download .nbib Format: NLM
Share it on

Cardiovasc Pathol. 1997 Nov-Dec;6(6):333-40. doi: 10.1016/S1054-8807(97)00031-8.

Lysosomal enzyme release from human neutrophils adherent to foreign material surfaces: enhanced release of elastase activity.

Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology

D J Erfle, J P Santerre, R S Labow

Affiliations

  1. Cardiovascular Devices Division and Department of Biochemistry, University of Ottawa Heart Institute, Ottawa, Ontario, Canada.
  2. Faculty of Dentistry, Department of Biomaterials, University of Toronto, Toronto, Ontario, Canada.

PMID: 25989886 DOI: 10.1016/S1054-8807(97)00031-8

Abstract

Neutrophils are the major phagocytic white blood cell present during the acute inflammatory response to cardiovascular medical devices and can become activated to release a wide variety of products that help mediate the overall host response. The purpose of this investigation was to develop an in vitro system to study the release of lysosomal enzymes from neutrophils adherent to biomaterial surfaces. Neutrophils isolated from peripheral human blood were allowed to adhere to different biomaterials and lysosomal enzyme release assessed by monitoring elastase-like activity in the supernatant. The number of adherent neutrophils with intact cytoplasmic membranes was estimated by extracting the cells and quantifying lactate dehydrogenase. Stimulated and non-stimulated neutrophils released significantly different amounts of elastase-like activity depending on the biomaterial surface to which they were adhered. The techniques developed in this study form the basis of an in vitro system for investigating the events associated with neutrophil/biomaterial interactions as well as a method for evaluating the white blood cell response to the materials used in circulatory support devices.

Copyright © 1997 Elsevier Science Inc. All rights reserved.

Publication Types