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Bruckner JJ, Zhan H, O'Connor-Giles KM. Advances in imaging ultrastructure yield new insights into presynaptic biology. Front Cell Neurosci. 2015;9:196doi: 10.3389/fncel.2015.00196.
Bruckner, J. J., Zhan, H., & O'Connor-Giles, K. M. (2015). Advances in imaging ultrastructure yield new insights into presynaptic biology. Frontiers in cellular neuroscience, 9196. https://doi.org/10.3389/fncel.2015.00196
Bruckner, Joseph J, et al. "Advances in imaging ultrastructure yield new insights into presynaptic biology." Frontiers in cellular neuroscience vol. 9 (2015): 196. doi: https://doi.org/10.3389/fncel.2015.00196
Bruckner JJ, Zhan H, O'Connor-Giles KM. Advances in imaging ultrastructure yield new insights into presynaptic biology. Front Cell Neurosci. 2015 May 22;9:196. doi: 10.3389/fncel.2015.00196. eCollection 2015. PMID: 26052269; PMCID: PMC4440913.
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Front Cell Neurosci. 2015 May 22;9:196. doi: 10.3389/fncel.2015.00196. eCollection 2015.

Advances in imaging ultrastructure yield new insights into presynaptic biology.

Frontiers in cellular neuroscience

Joseph J Bruckner, Hong Zhan, Kate M O'Connor-Giles

Affiliations

  1. Cell and Molecular Biology Training Program, University of Wisconsin-Madison Madison, WI, USA.
  2. Laboratory of Cell and Molecular Biology, University of Wisconsin-Madison Madison, WI, USA.
  3. Cell and Molecular Biology Training Program, University of Wisconsin-Madison Madison, WI, USA ; Laboratory of Cell and Molecular Biology, University of Wisconsin-Madison Madison, WI, USA ; Laboratory of Genetics, University of Wisconsin-Madison Madison, WI, USA.

PMID: 26052269 PMCID: PMC4440913 DOI: 10.3389/fncel.2015.00196

Abstract

Synapses are the fundamental functional units of neural circuits, and their dysregulation has been implicated in diverse neurological disorders. At presynaptic terminals, neurotransmitter-filled synaptic vesicles are released in response to calcium influx through voltage-gated calcium channels activated by the arrival of an action potential. Decades of electrophysiological, biochemical, and genetic studies have contributed to a growing understanding of presynaptic biology. Imaging studies are yielding new insights into how synapses are organized to carry out their critical functions. The development of techniques for rapid immobilization and preservation of neuronal tissues for electron microscopy (EM) has led to a new renaissance in ultrastructural imaging that is rapidly advancing our understanding of synapse structure and function.

Keywords: active zone; electron microscopy; presynapse; synaptic vesicle; ultrastructure

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