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Tokudome Y, Katayanagi M, Hashimoto F. Esterase Activity and Intracellular Localization in Reconstructed Human Epidermal Cultured Skin Models. Ann Dermatol. 2015;27(3):269-74doi: 10.5021/ad.2015.27.3.269.
Tokudome, Y., Katayanagi, M., & Hashimoto, F. (2015). Esterase Activity and Intracellular Localization in Reconstructed Human Epidermal Cultured Skin Models. Annals of dermatology, 27(3), 269-74. https://doi.org/10.5021/ad.2015.27.3.269
Tokudome, Yoshihiro, et al. "Esterase Activity and Intracellular Localization in Reconstructed Human Epidermal Cultured Skin Models." Annals of dermatology vol. 27,3 (2015): 269-74. doi: https://doi.org/10.5021/ad.2015.27.3.269
Tokudome Y, Katayanagi M, Hashimoto F. Esterase Activity and Intracellular Localization in Reconstructed Human Epidermal Cultured Skin Models. Ann Dermatol. 2015 Jun;27(3):269-74. doi: 10.5021/ad.2015.27.3.269. Epub 2015 May 29. PMID: 26082583; PMCID: PMC4466279.
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Ann Dermatol. 2015 Jun;27(3):269-74. doi: 10.5021/ad.2015.27.3.269. Epub 2015 May 29.

Esterase Activity and Intracellular Localization in Reconstructed Human Epidermal Cultured Skin Models.

Annals of dermatology

Yoshihiro Tokudome, Mishina Katayanagi, Fumie Hashimoto

Affiliations

  1. Laboratory of Dermatological Physiology, Faculty of Pharmaceutical Sciences, Josai University, Sakado, Japan.

PMID: 26082583 PMCID: PMC4466279 DOI: 10.5021/ad.2015.27.3.269

Abstract

BACKGROUND: Reconstructed human epidermal culture skin models have been developed for cosmetic and pharmaceutical research.

OBJECTIVE: This study evaluated the total and carboxyl esterase activities (i.e., Km and Vmax , respectively) and localization in two reconstructed human epidermal culture skin models (LabCyte EPI-MODEL [Japan Tissue Engineering] and EpiDerm [MatTek/Kurabo]). The usefulness of the reconstruction cultured epidermis was also verified by comparison with human and rat epidermis.

METHODS: Homogenized epidermal samples were fractioned by centrifugation. p-nitrophenyl acetate and 4-methylumbelliferyl acetate were used as substrates of total esterase and carboxyl esterase, respectively.

RESULTS: Total and carboxyl esterase activities were present in the reconstructed human epidermal culture skin models and were localized in the cytosol. Moreover, the activities and localization were the same as those in human and rat epidermis.

CONCLUSION: LabCyte EPI-MODEL and EpiDerm are potentially useful for esterase activity prediction in human epidermis.

Keywords: Carboxyl esterase; Localization; Reconstructed human epidermal culture skin model; Skin esterase; Total esterase

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