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Pohanka M. Postponed effect of neostigmine on oxidative homeostasis. Interdiscip Toxicol. 2014;7(3):134-8doi: 10.2478/intox-2014-0018.
Pohanka, M. (2014). Postponed effect of neostigmine on oxidative homeostasis. Interdisciplinary toxicology, 7(3), 134-8. https://doi.org/10.2478/intox-2014-0018
Pohanka, Miroslav. "Postponed effect of neostigmine on oxidative homeostasis." Interdisciplinary toxicology vol. 7,3 (2014): 134-8. doi: https://doi.org/10.2478/intox-2014-0018
Pohanka M. Postponed effect of neostigmine on oxidative homeostasis. Interdiscip Toxicol. 2014 Sep;7(3):134-8. doi: 10.2478/intox-2014-0018. Epub 2014 Dec 30. PMID: 26109890; PMCID: PMC4434106.
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Interdiscip Toxicol. 2014 Sep;7(3):134-8. doi: 10.2478/intox-2014-0018. Epub 2014 Dec 30.

Postponed effect of neostigmine on oxidative homeostasis.

Interdisciplinary toxicology

Miroslav Pohanka

Affiliations

  1. Faculty of Military Health Sciences, University of Defense, Hradec Kralove, Czech Republic ; Karel English College in Brno, Brno, Czech Republic.

PMID: 26109890 PMCID: PMC4434106 DOI: 10.2478/intox-2014-0018

Abstract

Cholinesterases are enzymes able to hydrolyze the neurotransmitter acetylcholine and thus to terminate transmission. Once the enzymes are inhibited, excitotoxicity can appear in the adjacent cells. It is well known that oxidative stress is involved in the toxicity of cholinesterase inhibitors. Commonly, stress follows inhibition of cholinesterases and disappears shortly afterwards. In the present experiment, it was decided to test the impact of an inhibitor, neostigmine, on oxidative stress in BALB/c mice after a longer interval. The animals were sacrificed three days after onset of the experiment and spleens and livers were collected. Reduced glutathione (GSH), glutathione reductase (GR), glutathione S-transferase (GST), thiobarbituric acid reactive substances (TBARS), ferric reducing antioxidant power (FRAP), caspase-3 and activity of acetylcholinesterase (AChE) were assayed. The tested markers were not altered with exceptions of FRAP. The FRAP values indicate accumulation of low molecular weight antioxidants in the examined organs. The role of low molecular weight antioxidants in the toxicity of AChE inhibitors is discussed.

Keywords: acetylcholinesterase; antioxidant; butyrylcholinesterase; excitotoxicity; glutathione; inhibitor; neostigmine; oxidative stress

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