Display options
Cite
Ayad E, Mansy M, Elwi D, et al. Comparative study between quantitative digital image analysis and fluorescence in situ hybridization of breast cancer equivocal human epidermal growth factor receptors 2 score 2(+) cases. J Pathol Inform. 2015;6:31doi: 10.4103/2153-3539.158066.
Ayad, E., Mansy, M., Elwi, D., Salem, M., Salama, M., & Kayser, K. (2015). Comparative study between quantitative digital image analysis and fluorescence in situ hybridization of breast cancer equivocal human epidermal growth factor receptors 2 score 2(+) cases. Journal of pathology informatics, 631. https://doi.org/10.4103/2153-3539.158066
Ayad, Essam, et al. "Comparative study between quantitative digital image analysis and fluorescence in situ hybridization of breast cancer equivocal human epidermal growth factor receptors 2 score 2(+) cases." Journal of pathology informatics vol. 6 (2015): 31. doi: https://doi.org/10.4103/2153-3539.158066
Ayad E, Mansy M, Elwi D, Salem M, Salama M, Kayser K. Comparative study between quantitative digital image analysis and fluorescence in situ hybridization of breast cancer equivocal human epidermal growth factor receptors 2 score 2(+) cases. J Pathol Inform. 2015 Jun 03;6:31. doi: 10.4103/2153-3539.158066. eCollection 2015. PMID: 26110098; PMCID: PMC4470009.
Copy Download .nbib Format: NLM
Share it on

J Pathol Inform. 2015 Jun 03;6:31. doi: 10.4103/2153-3539.158066. eCollection 2015.

Comparative study between quantitative digital image analysis and fluorescence in situ hybridization of breast cancer equivocal human epidermal growth factor receptors 2 score 2(+) cases.

Journal of pathology informatics

Essam Ayad, Mina Mansy, Dalal Elwi, Mostafa Salem, Mohamed Salama, Klaus Kayser

Affiliations

  1. Department of Pathology, Faculty of Medicine, Cairo University, Cairo, Egypt.
  2. Department of Pathology, University of Utah and ARUP Reference Lab, Utah, USA.
  3. Department of Pathology, Humbold University Berlin, Berlin, Germany.

PMID: 26110098 PMCID: PMC4470009 DOI: 10.4103/2153-3539.158066

Abstract

BACKGROUND: Optimization of workflow for breast cancer samples with equivocal human epidermal growth factor receptors 2 (HER2)/neu score 2(+) results in routine practice, remains to be a central focus of the on-going efforts to assess HER2 status. According to the College of American Pathologists/American Society of Clinical Oncology guidelines equivocal HER2/neu score 2(+) cases are subject for further testing, usually by fluorescence in situ hybridization (FISH) investigations. It still remains on open question, whether quantitative digital image analysis of HER2 immunohistochemistry (IHC) stained slides can assist in further refining the HER2 score 2(+).

AIM OF THIS WORK: To assess utility of quantitative digital analysis of IHC stained slides and compare its performance to FISH in cases of breast cancer with equivocal HER2 score 2(+).

MATERIALS AND METHODS: Fifteen specimens (previously diagnosed as breast cancer and was evaluated as HER 2(-) score 2(+)) represented the study population. Contemporary new cuts were prepared for re-evaluation of HER2 immunohistochemical studies and FISH examination. All the cases were digitally scanned by iScan (Produced by BioImagene [Now Roche-Ventana]). The IHC signals of HER2 were measured using an automated image analyzing system (MECES, www.Diagnomx.eu/meces). Finally, a comparative study was done between the results of the FISH and the quantitative analysis of the virtual slides.

RESULTS: Three out of the 15 cases with equivocal HER2 score 2(+), turned out to be positive (3(+)) by quantitative digital analysis, and 12 were found to be negative in FISH too. Two of these three positive cases proved to be positive with FISH, and only one was negative.

CONCLUSIONS: Quantitative digital analysis is highly sensitive and relatively specific when compared to FISH in detecting HER2/neu overexpression. Therefore, it represents a potential reliable substitute for FISH in breast cancer cases, which desire further refinement of equivocal IHC results.

Keywords: Breast cancer; fluorescence in situ hybridization; human epidermal growth factor receptors 2; quantitative digital image analysis

References

  1. Am J Clin Pathol. 2012 Feb;137(2):270-6 - PubMed
  2. J Pathol Inform. 2013 Jul 31;4:19 - PubMed
  3. J Clin Oncol. 2007 Jan 1;25(1):118-45 - PubMed
  4. J Clin Oncol. 2010 Oct 1;28(28):4289-92 - PubMed
  5. J Natl Cancer Inst. 2012 Jun 20;104(12):957-8 - PubMed
  6. J Clin Oncol. 2009 Nov 20;27(33):5492-4 - PubMed
  7. Cancer. 2009 Nov 15;115(22):5166-74 - PubMed
  8. Histopathology. 2010 Jul;57(1):27-38 - PubMed
  9. Am J Clin Pathol. 2001 Oct;116(4):495-503 - PubMed
  10. Arch Pathol Lab Med. 2009 Sep;133(9):1413-9 - PubMed
  11. CMAJ. 2007 May 8;176(10):1429-34 - PubMed
  12. BMC Cancer. 2009 May 29;9:165 - PubMed
  13. Arch Pathol Lab Med. 2010 May;134(5):728-34 - PubMed
  14. IEEE Trans Med Imaging. 2009 Jun;28(6):916-25 - PubMed
  15. J Clin Oncol. 2013 Nov 1;31(31):3997-4013 - PubMed
  16. Am J Clin Pathol. 2004 May;121(5):620-30 - PubMed
  17. Histopathology. 2006 Feb;48(3):258-67 - PubMed

Publication Types