Display options
Cite
Zeller E, Schollenberger M, Kühn I, et al. Hydrolysis of phytate and formation of inositol phosphate isomers without or with supplemented phytases in different segments of the digestive tract of broilers. J Nutr Sci. 2015;4:e1doi: 10.1017/jns.2014.62.
Zeller, E., Schollenberger, M., Kühn, I., & Rodehutscord, M. (2015). Hydrolysis of phytate and formation of inositol phosphate isomers without or with supplemented phytases in different segments of the digestive tract of broilers. Journal of nutritional science, 4e1. https://doi.org/10.1017/jns.2014.62
Zeller, Ellen, et al. "Hydrolysis of phytate and formation of inositol phosphate isomers without or with supplemented phytases in different segments of the digestive tract of broilers." Journal of nutritional science vol. 4 (2015): e1. doi: https://doi.org/10.1017/jns.2014.62
Zeller E, Schollenberger M, Kühn I, Rodehutscord M. Hydrolysis of phytate and formation of inositol phosphate isomers without or with supplemented phytases in different segments of the digestive tract of broilers. J Nutr Sci. 2015 Jan 26;4:e1. doi: 10.1017/jns.2014.62. eCollection 2015. PMID: 26090091; PMCID: PMC4463934.
Copy Download .nbib Format: NLM
Share it on

J Nutr Sci. 2015 Jan 26;4:e1. doi: 10.1017/jns.2014.62. eCollection 2015.

Hydrolysis of phytate and formation of inositol phosphate isomers without or with supplemented phytases in different segments of the digestive tract of broilers.

Journal of nutritional science

Ellen Zeller, Margit Schollenberger, Imke Kühn, Markus Rodehutscord

Affiliations

  1. Institut für Tierernährung, Universität Hohenheim , 70599 Stuttgart , Germany.
  2. AB Vista Feed Ingredients , 64293 Darmstadt , Germany.

PMID: 26090091 PMCID: PMC4463934 DOI: 10.1017/jns.2014.62

Abstract

The objective was to characterise degradation of myo-inositol 1,2,3,4,5,6-hexakis (dihydrogen phosphate) (InsP6) and formation of inositol phosphate (InsP) isomers in different segments of the broiler digestive tract. Influence of an Aspergillus niger (PhyA) and two Escherichia coli-derived (PhyE1 and PhyE2) phytases was also investigated. A total of 600 16-d-old broilers were allocated to forty floor pens (ten pens per treatment). Low-P (5·2 g/kg DM) maize-soyabean meal-based diets were fed without (basal diet; BD) or with a phytase added. On day 25, digesta from different digestive tract segments were pooled per segment on a pen-basis, freeze-dried and analysed for P, InsP isomers and the marker TiO2. InsP6 degradation until the lower ileum (74 %) in BD-fed birds showed a high potential of broilers and their gut microbiota to hydrolyse InsP6 in low-P diets. Different InsP patterns in different gut segments suggested the involvement of phosphatases of different origin. Supplemented phytases increased InsP6 hydrolysis in the crop (P < 0·01) but not in the lower ileum. Measurements in the crop and proventriculus/gizzard confirmed published in vitro degradation pathways of 3- and 6-phytases for the first time. In the intestinal segments, specifically formed InsP4-5 isomers of supplemented phytases were still present, indicating further activity of these enzymes. Myo-inositol tetrakisphosphate (InsP4) accumulation differed between PhyE1 and PhyE2 compared with PhyA in the anterior segments of the gut (P < 0·01). Thus, the hydrolytic cleavage of the first phosphate group is not the only limiting step in phytate degradation in broilers.

Keywords: BD, basal diet; Broilers; Inositol phosphate isomers; InsP, inositol phosphate; InsP3, myo-inositol trisphosphate; InsP4, myo-inositol tetrakisphosphate; InsP5, myo-inositol pentakisphosphate; InsP6, myo-inositol 1,2,3,4,5,6-hexakis (dihydrogen phosphate); PhyA, Aspergillus-derived phytase Finase® P; PhyE1, Escherichia coli-derived phytase Quantum®; PhyE2, E. coli-derived phytase Quantum® Blue; Phytases; Phytate hydrolysis; tP, total P

References

  1. Science. 1999 Mar 26;283(5410):2015 - PubMed
  2. J Agric Food Chem. 1999 Apr;47(4):1385-8 - PubMed
  3. J Nutr. 2000 Aug;130(8):2020-5 - PubMed
  4. J Biotechnol. 2001 Nov 17;84(1):53-62 - PubMed
  5. Arch Tierernahr. 2000;53(4):353-73 - PubMed
  6. Folia Microbiol (Praha). 2000;45(2):128-32 - PubMed
  7. J Agric Food Chem. 2001 May;49(5):2234-40 - PubMed
  8. J Appl Microbiol. 2002;93(4):668-74 - PubMed
  9. Arch Tierernahr. 2001;55(4):255-80 - PubMed
  10. Br Poult Sci. 2002 Dec;43(5 Suppl):662-8 - PubMed
  11. Can J Microbiol. 2002 Nov;48(11):986-94 - PubMed
  12. Poult Sci. 2003 Jul;82(7):1140-8 - PubMed
  13. J Chromatogr A. 2003 Nov 7;1018(1):41-52 - PubMed
  14. Appl Environ Microbiol. 2003 Nov;69(11):6816-24 - PubMed
  15. J Agric Food Chem. 2003 Jul 30;51(16):4687-93 - PubMed
  16. Appl Environ Microbiol. 2004 May;70(5):3041-6 - PubMed
  17. Poult Sci. 2004 Aug;83(8):1358-67 - PubMed
  18. Br Poult Sci. 2007 Feb;48(1):64-70 - PubMed
  19. Food Microbiol. 2008 Feb;25(1):169-76 - PubMed
  20. J Environ Qual. 2008 Feb 11;37(2):494-500 - PubMed
  21. Int J Biochem Cell Biol. 2008;40(10):2053-64 - PubMed
  22. Poult Sci. 2008 Dec;87(12):2466-76 - PubMed
  23. Int J Food Microbiol. 2009 Jul 31;133(1-2):129-34 - PubMed
  24. Int J Food Microbiol. 2009 Sep 30;135(1):7-14 - PubMed
  25. J Anim Sci. 2012 Jun;90(6):1824-32 - PubMed
  26. J Biosci Bioeng. 2012 May;113(5):562-7 - PubMed
  27. Poult Sci. 2012 Mar;91(3):693-700 - PubMed
  28. Poult Sci. 2012 Apr;91(4):965-71 - PubMed
  29. Poult Sci. 2012 Sep;91(9):2201-9 - PubMed
  30. Arch Anim Nutr. 2012 Dec;66(6):431-44 - PubMed
  31. Braz J Microbiol. 2009 Oct;40(4):795-807 - PubMed
  32. Arch Anim Nutr. 2009;63(4):337-42 - PubMed
  33. Anal Biochem. 1988 Nov 15;175(1):162-6 - PubMed
  34. Aust J Biol Sci. 1970 Dec;23(6):1207-20 - PubMed
  35. Plant Physiol. 1994 Dec;106(4):1489-95 - PubMed
  36. Arch Biochem Biophys. 1993 May 15;303(1):107-13 - PubMed
  37. Biochem J. 1995 Dec 15;312 ( Pt 3):907-10 - PubMed
  38. Arch Biochem Biophys. 1997 May 15;341(2):201-6 - PubMed
  39. Poult Sci. 1998 Apr;77(4):557-63 - PubMed
  40. Appl Environ Microbiol. 1998 Jun;64(6):2079-85 - PubMed
  41. Appl Environ Microbiol. 1999 Feb;65(2):359-66 - PubMed

Publication Types