Display options
Cite
Orrù R, Atzori E, Padiglia A. Development of a molecular method for the rapid screening and identification of the three functionally relevant polymorphisms in the human TAS2R38 receptor gene in studies of sensitivity to the bitter taste of PROP. Springerplus. 2015;4:246doi: 10.1186/s40064-015-1045-0.
Orrù, R., Atzori, E., & Padiglia, A. (2015). Development of a molecular method for the rapid screening and identification of the three functionally relevant polymorphisms in the human TAS2R38 receptor gene in studies of sensitivity to the bitter taste of PROP. SpringerPlus, 4246. https://doi.org/10.1186/s40064-015-1045-0
Orrù, Roberto, et al. "Development of a molecular method for the rapid screening and identification of the three functionally relevant polymorphisms in the human TAS2R38 receptor gene in studies of sensitivity to the bitter taste of PROP." SpringerPlus vol. 4 (2015): 246. doi: https://doi.org/10.1186/s40064-015-1045-0
Orrù R, Atzori E, Padiglia A. Development of a molecular method for the rapid screening and identification of the three functionally relevant polymorphisms in the human TAS2R38 receptor gene in studies of sensitivity to the bitter taste of PROP. Springerplus. 2015 Jun 09;4:246. doi: 10.1186/s40064-015-1045-0. eCollection 2015. PMID: 26090297; PMCID: PMC4467798.
Copy Download .nbib Format: NLM
Share it on

Springerplus. 2015 Jun 09;4:246. doi: 10.1186/s40064-015-1045-0. eCollection 2015.

Development of a molecular method for the rapid screening and identification of the three functionally relevant polymorphisms in the human TAS2R38 receptor gene in studies of sensitivity to the bitter taste of PROP.

SpringerPlus

Roberto Orrù, Elena Atzori, Alessandra Padiglia

Affiliations

  1. Department of Life and Environment Sciences, University of Cagliari, 09042 Monserrato, CA Italy.

PMID: 26090297 PMCID: PMC4467798 DOI: 10.1186/s40064-015-1045-0

Abstract

The objective of this work was to develop a rapid screening method to identify the three single nucleotide polymorphisms (SNPs) in the TAS2R38 gene, with the aim of providing a significant contribution to studies designed to assess sensitivity to the bitter taste of 6-n-propylthiouracil (PROP). Specifically, the objective of this study was to characterize the TAS2R38 gene haplotypes in a group of 60 subjects with variable sensitivity to PROP and preliminarily genotyped for the rs2274333 allele (A/G) of carbonic anhydrase isoform VI gene (CA6). The molecular characterization of the TAS2R38 gene was conducted using the PCR-restriction fragment length polymorphism technique after creating artificial restriction sites upstream or downstream of the SNPs, as none of the three polymorphisms contributes to the formation of a restriction site for a specific endonuclease. The results indicate that the method described in this paper could be a valid and simple experimental strategy to identify genetic differences related to taste sensitivity to bitter taste, and could be applied as a nutrigenetics test in studies aimed at understanding people's eating behaviors.

Keywords: Bitter taste; CA6; PCR-RFLP; PROP taster status; TAS2R38

References

  1. Physiol Behav. 2006 Feb 28;87(2):304-13 - PubMed
  2. Physiol Behav. 2011 Oct 24;104(5):1065-71 - PubMed
  3. Chem Senses. 2004 Oct;29(8):697-702 - PubMed
  4. PLoS One. 2013 Sep 09;8(9):e74151 - PubMed
  5. J Neurosci. 2003 Aug 13;23 (19):7376-80 - PubMed
  6. Am J Clin Nutr. 2005 May;81(5):1005-11 - PubMed
  7. Hum Genet. 2003 May;112(5-6):567-72 - PubMed
  8. Neurosci Lett. 2011 Nov 14;505(2):160-4 - PubMed
  9. Physiol Rev. 1996 Jul;76(3):718-66 - PubMed
  10. Am J Clin Nutr. 2006 Jul;84(1):245-51 - PubMed
  11. Transfusion. 2012 May;52(5):1092-6 - PubMed
  12. Am J Clin Nutr. 2010 Sep;92(3):539-45 - PubMed
  13. Am J Hum Genet. 2004 Apr;74(4):637-46 - PubMed
  14. Ann N Y Acad Sci. 1998 Nov 30;855:802-4 - PubMed
  15. Chem Senses. 2000 Aug;25(4):447-60 - PubMed
  16. J Am Diet Assoc. 2007 Aug;107(8):1340-8 - PubMed
  17. Clin Genet. 2005 Apr;67(4):275-80 - PubMed
  18. PLoS One. 2014 Mar 13;9(3):e91716 - PubMed
  19. Appetite. 2002 Feb;38(1):3-12 - PubMed
  20. PLoS One. 2011;6(11):e27745 - PubMed
  21. Physiol Behav. 2001 Jul;73(4):571-7 - PubMed
  22. Alcohol Clin Exp Res. 2004 Nov;28(11):1629-37 - PubMed
  23. Annu Rev Nutr. 2008;28:367-88 - PubMed
  24. Genet Test Mol Biomarkers. 2011 Oct;15(10):721-4 - PubMed
  25. J Comp Neurol. 1984 Feb 1;222(4):560-77 - PubMed
  26. Nutr Res. 2011 Aug;31(8):594-600 - PubMed
  27. J Membr Biol. 1989 Nov;112(1):1-8 - PubMed
  28. J Clin Microbiol. 1982 Sep;16(3):473-7 - PubMed
  29. Science. 2003 Feb 21;299(5610):1221-5 - PubMed
  30. Chem Senses. 2007 Mar;32(3):225-36 - PubMed
  31. Curr Biol. 2005 Feb 22;15(4):322-7 - PubMed
  32. Nucleic Acids Res. 2012 Jan;40(Database issue):D413-9 - PubMed
  33. J Biol Chem. 2002 Jan 4;277(1):1-4 - PubMed
  34. PLoS One. 2015 Jan 14;10(1):e0116479 - PubMed
  35. Cell Mol Life Sci. 2006 Jul;63(13):1501-9 - PubMed
  36. Physiol Behav. 2014 Apr 10;128:148-54 - PubMed

Publication Types