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Ramazanzadeh R, Rouhi S, Shakib P, et al. Molecular Characterization of Vibrio cholerae Isolated From Clinical Samples in Kurdistan Province, Iran. Jundishapur J Microbiol. 2015;8(5):e18119doi: 10.5812/jjm.8(5)2015.18119.
Ramazanzadeh, R., Rouhi, S., Shakib, P., Shahbazi, B., Bidarpour, F., & Karimi, M. (2015). Molecular Characterization of Vibrio cholerae Isolated From Clinical Samples in Kurdistan Province, Iran. Jundishapur journal of microbiology, 8(5), e18119. https://doi.org/10.5812/jjm.8(5)2015.18119
Ramazanzadeh, Rashid, et al. "Molecular Characterization of Vibrio cholerae Isolated From Clinical Samples in Kurdistan Province, Iran." Jundishapur journal of microbiology vol. 8,5 (2015): e18119. doi: https://doi.org/10.5812/jjm.8(5)2015.18119
Ramazanzadeh R, Rouhi S, Shakib P, Shahbazi B, Bidarpour F, Karimi M. Molecular Characterization of Vibrio cholerae Isolated From Clinical Samples in Kurdistan Province, Iran. Jundishapur J Microbiol. 2015 May 31;8(5):e18119. doi: 10.5812/jjm.8(5)2015.18119. eCollection 2015 May. PMID: 26060565; PMCID: PMC4458358.
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Jundishapur J Microbiol. 2015 May 31;8(5):e18119. doi: 10.5812/jjm.8(5)2015.18119. eCollection 2015 May.

Molecular Characterization of Vibrio cholerae Isolated From Clinical Samples in Kurdistan Province, Iran.

Jundishapur journal of microbiology

Rashid Ramazanzadeh, Samaneh Rouhi, Pegah Shakib, Babak Shahbazi, Farzam Bidarpour, Mohammad Karimi

Affiliations

  1. Cellular and Molecular Research Center, Kurdistan University of Medical Sciences, Sanandaj, IR Iran ; Department of Microbiology, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, IR Iran.
  2. Student Research Committee, Kurdistan University of Medical Sciences, Sanandaj, IR Iran.
  3. Department of Microbiology, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, IR Iran.
  4. Deputy of Public Health Affairs, Kurdistan University of Medical Sciences, Sanandaj, IR Iran.

PMID: 26060565 PMCID: PMC4458358 DOI: 10.5812/jjm.8(5)2015.18119

Abstract

BACKGROUND: Vibrio cholerae causes diarrhoeal disease that afflicts thousands of people annually. V. cholerae is classified on the basis of somatic antigens into serovars or serogroups and there are at least 200 known serogroup. Two serogroups, O1 and O139 have been associated with epidemic diseases. Virulence genes of these bacteria are OmpW, ctxA and tcpA.

OBJECTIVES: Due to the importance of V. cholerae infection and developing molecular diagnostics of this organism in medical and microbiology sciences, this study aimed to describe molecular characterization of V. cholerae isolated from clinical samples using a molecular method.

MATERIALS AND METHODS: In this study, 48 samples were provided during summer 2013 (late August and early September) by reference laboratory. Samples were assessed using biochemical tests initially. The primer of OmpW, ctxA and tcpA genes was used in Polymerase Chain Reaction (PCR) protocols. Enterobacterial Repetitive Intergenic Consensus (ERIC)-PCR and Repetitive Extragenic Palindromic (REP)-PCR methods were used to subtype V. cholerae.

RESULTS: In this study, from a total of 48 clinical stool samples 39 (81.2 %) were positive for V. cholerae in biochemical tests and bacteria culture tests. The PCR results showed that of 39 positive isolates 35 (89.7%), 34 (87.1%) and 37 (94.8%) were positive for ctxA, tcpA and OmpW gene, respectively. Also, in the REP-PCR method with ERIC primer strains were divided into 10 groups. In the REP-PCR method with REP primer, strains were divided into 13 groups.

CONCLUSIONS: Polymerase chain reaction has specificity and accuracy for identification of the organism and is able to differentiate biotypes. Enterobacterial repetitive intergenic consensus sequence is one of the informative and discriminative methods for the analysis of V. cholerae diversity. The REP-PCR is a less informative and discriminative method compared to other methods for the analysis of V. cholerae diversity.

Keywords: Clinical Samples; Molecular Characterization; Vibrio cholerae; Virulence Genes

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