Asian Pac J Trop Med. 2015 Jun;8(6):485-8. doi: 10.1016/j.apjtm.2015.05.004. Epub 2015 Jun 25.
Effect of bortezomib on migration and invasion in cervical carcinoma HeLa cell.
Asian Pacific journal of tropical medicine
Chong Shi, Guo-Bin Zhang, Shu-Wang Yin
Affiliations
Affiliations
- Department of Genetic Reproduction, Maternal and Child Health Hospital, Tangshan 063000, China. Electronic address: [email protected].
- Department of Graduate, Hebei United University, Tangshan 063000, China.
- Pharmacy Department, Affiliated Hospital of North China Coal Medical University, Tangshan 063000, China.
PMID: 26194835
DOI: 10.1016/j.apjtm.2015.05.004
Abstract
OBJECTIVE: To explore the effect of bortezomib on migration and invasion of cervical carcinoma HeLa cell and specific molecular mechanism.
METHODS: The effect of bortezomib on the viability of HeLa cell was measured by MTT assay. The effect of bortezomib on cell migration and invasion was measured by Transwell assay and invasion experiment respectively. The activation of Akt/mTOR signaling pathway and expression level of MMP2, MMP9 were assayed by western blot.
RESULTS: MTT assay indicated bortezomib (2.5 μM, 5 μM, 10 μM) could inhibit HeLa cell viability, and the inhibitory rate was highest at 48 h. Transwell assay and invasion experiment results showed that bortezomib inhibited HeLa cell migration and invasion. Western blotting assays presented bortezomib could suppress the phosphorylation of Akt and mTOR, and down-regulate the expression of MMP2 and MMP9.
CONCLUSIONS: These results suggested bortezomib could inhibit migration and invasion in cervical carcinoma HeLa cell, which might be related to Akt/mTOR signal pathway.
Copyright © 2015 Hainan Medical College. Production and hosting by Elsevier B.V. All rights reserved.
Keywords: Akt/mTOR signal pathway; Bortezomib; Cervical carcinoma HeLa cell; Invasion; Migration
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