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Ubhayawardana DL, Kottahachchi J, Weerasekera MM, et al. Residual bioburden in reprocessed side-view endoscopes used for endoscopic retrograde cholangiopancreatography (ERCP). Endosc Int Open. 2013;1(1):12-6doi: 10.1055/s-0033-1359234.
Ubhayawardana, D. L., Kottahachchi, J., Weerasekera, M. M., Wanigasooriya, I. W., Fernando, S. S., & De Silva, M. (2013). Residual bioburden in reprocessed side-view endoscopes used for endoscopic retrograde cholangiopancreatography (ERCP). Endoscopy international open, 1(1), 12-6. https://doi.org/10.1055/s-0033-1359234
Ubhayawardana, D L N L, et al. "Residual bioburden in reprocessed side-view endoscopes used for endoscopic retrograde cholangiopancreatography (ERCP)." Endoscopy international open vol. 1,1 (2013): 12-6. doi: https://doi.org/10.1055/s-0033-1359234
Ubhayawardana DL, Kottahachchi J, Weerasekera MM, Wanigasooriya IW, Fernando SS, De Silva M. Residual bioburden in reprocessed side-view endoscopes used for endoscopic retrograde cholangiopancreatography (ERCP). Endosc Int Open. 2013 Dec;1(1):12-6. doi: 10.1055/s-0033-1359234. PMID: 26135507; PMCID: PMC4440374.
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Endosc Int Open. 2013 Dec;1(1):12-6. doi: 10.1055/s-0033-1359234.

Residual bioburden in reprocessed side-view endoscopes used for endoscopic retrograde cholangiopancreatography (ERCP).

Endoscopy international open

D L N L Ubhayawardana, J Kottahachchi, M M Weerasekera, I W M P Wanigasooriya, S S N Fernando, M De Silva

Affiliations

  1. Department of Microbiology, Faculty of Medical Sciences, University of Sri Jayewardenepura, Nugegoda, Sri Lanka.
  2. Endo-Therapy Unit, Colombo South Teaching Hospital, Kalubowila, Sri Lanka.

PMID: 26135507 PMCID: PMC4440374 DOI: 10.1055/s-0033-1359234

Abstract

BACKGROUND AND STUDY AIM: Worldwide some endoscopy units routinely continue to use manual reprocessing techniques for disinfection of side-view endoscopes. The aim of this study was to evaluate the outcome quality of manual reprocessing techniques for removal and inactivation of the bioburden from side-view endoscopes used for endoscopic retrograde cholangiopancreatography (ERCP) in a tertiary referral endotherapy unit in Sri Lanka.

METHODS: 102 samples obtained from two different flexible side-view endoscopes (Olympus TJF Q 180V and Olympus TJF 160 R) were tested for microbial growth. Three samples were collected each time; one swab from the tip before and another after manual reprocessing. The third sample was collected by flushing the working channel with sterile normal saline after manual reprocessing. Microorganisms were identified by culturing the samples.

RESULT: : After reprocessing, culture-positive rates were 20 % and 9 % for the samples obtained from the tip and the working channel of the side-view endoscopes, respectively. Klebsiella spp. and Candida spp. were found to be the commonest microorganisms in the samples from the tips and from the working channels, respectively, of the reprocessed side-view endoscopes.

CONCLUSION: There is a high culture-positive rate after reprocessing of the side-view endoscopes using the manual reprocessing procedure, despite strict adherence to the protocol for reprocessing.

References

  1. Am J Infect Control. 2006 Jun;34(5):274-80 - PubMed
  2. Clin Endosc. 2011 Dec;44(2):109-15 - PubMed
  3. Gastrointest Endosc. 2001 Dec;54(6):824-8 - PubMed
  4. Gastrointest Endosc. 1998 Aug;48(2):137-42 - PubMed
  5. Gastrointest Endosc Clin N Am. 2000 Apr;10(2):233-44 - PubMed
  6. Gastrointest Endosc. 2003 May;57(6):695-711 - PubMed
  7. Endoscopy. 2008 Nov;40(11):939-57 - PubMed
  8. World J Gastroenterol. 2006 Jul 7;12(25):3953-64 - PubMed
  9. Endoscopy. 2010 Nov;42(11):895-9 - PubMed
  10. Endoscopy. 2010 Nov;42(11):957-9 - PubMed
  11. Curr Opin Infect Dis. 2005 Aug;18(4):326-30 - PubMed
  12. Gastroenterol Nurs. 1999 Mar-Apr;22(2):63-8 - PubMed
  13. Gastrointest Endosc. 2010 Oct;72(4):675-80 - PubMed
  14. J Hosp Infect. 1994 Jan;26(1):15-26 - PubMed
  15. Infect Control Hosp Epidemiol. 2002 Apr;23(4):198-206 - PubMed
  16. World J Gastroenterol. 2006 Jul 14;12(26):4199-202 - PubMed
  17. Am J Gastroenterol. 2003 Jan;98(1):77-81 - PubMed
  18. J Hosp Infect. 2004 Apr;56 Suppl 2:S27-39 - PubMed
  19. Endoscopy. 2007 Feb;39(2):175-81 - PubMed
  20. J Gastroenterol Hepatol. 2008 Jul;23(7 Pt 1):1069-74 - PubMed
  21. Am J Infect Control. 2013 Aug;41(8):734-6 - PubMed
  22. Gastrointest Endosc. 2011 Jun;73(6):1075-84 - PubMed
  23. Gastrointest Endosc Clin N Am. 2000 Apr;10(2):215-32 - PubMed

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