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J Basic Clin Pharm. 2015 Jun;6(3):77-83. doi: 10.4103/0976-0105.160738.

Antiarthritic activity of a polyherbal formulation against Freund's complete adjuvant induced arthritis in Female Wistar rats.

Journal of basic and clinical pharmacy

R Ramesh Petchi, S Parasuraman, C Vijaya, S V Gopala Krishna, M Kiran Kumar


  1. Department of Pharmacology, Vasavi Institute of Pharmaceutical Sciences, Peddapalli, Kadapa, Andhra Pradesh, India.
  2. Unit of Pharmacology, Faculty of Pharmacy, AIMST University, Bedong 08100, Kedah, Malaysia.
  3. Department of Pharmacology, Ultra College of Pharmacy, Madurai, Tamil Nadu, India.

PMID: 26229343 PMCID: PMC4513335 DOI: 10.4103/0976-0105.160738


OBJECTIVES: To formulate a polyherbal formulation and evaluate its antiarthritic activity against Freund's complete adjuvant induced arthritis in Female Wistar rats.

MATERIALS AND METHODS: Glycosmis pentaphylla, Tridax procumbens, and Mangifera indica are well-known plants available throughout India and they are commonly used for the treatment of various diseases including arthritis. The polyherbal formulation was formulated using the ethanol extracts of the stem bark of G. pentaphylla , whole plant of T. procumbens, and leaves of M. indica. The polyherbal formulation contains the ethanol extracts of G. pentaphylla, T. procumbens, and M. indica in the ratio of 2:2:1. The quality of the finished product was evaluated as per the World Health Organization's guidelines for the quality control of herbal materials. Arthritis was induced in female Wistar rats using Freund's complete adjuvant (FCA), and the antiarthritic effect of polyherbal formulation was studied at doses of 250 and 500 mg/kg. The effects were compared with those of indomethacin (10 mg/kg). At the end of the study, blood samples were collected for biochemical and hematological analysis. The radiological examination was carried out before terminating the study.

RESULTS: Polyherbal formulation showed significant antiarthritic activity at 250 and 500 mg/kg, respectively, and this effect was comparable with that of indomethacin. The antiarthritic activity of polyherbal formulation is supported by biochemical and hematological analysis.

CONCLUSION: The polyherbal formulation showed signinicant antiarthritic activity against FCA-induced arthritis in female Wistar rats.

Keywords: Arthritis; Fingerprint analysis; Glycosmis pentaphylla; Mangifera indica; Tridax procumbens


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