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Nakai K, Kawato T, Morita T, et al. Angiotensin II suppresses osteoblastic differentiation and mineralized nodule formation via AT1 receptor in ROS17/2.8 cells. Arch Med Sci. 2015;11(3):628-37doi: 10.5114/aoms.2015.52369.
Nakai, K., Kawato, T., Morita, T., Yamazaki, Y., Tanaka, H., Tonogi, M., Oki, H., & Maeno, M. (2015). Angiotensin II suppresses osteoblastic differentiation and mineralized nodule formation via AT1 receptor in ROS17/2.8 cells. Archives of medical science : AMS, 11(3), 628-37. https://doi.org/10.5114/aoms.2015.52369
Nakai, Kumiko, et al. "Angiotensin II suppresses osteoblastic differentiation and mineralized nodule formation via AT1 receptor in ROS17/2.8 cells." Archives of medical science : AMS vol. 11,3 (2015): 628-37. doi: https://doi.org/10.5114/aoms.2015.52369
Nakai K, Kawato T, Morita T, Yamazaki Y, Tanaka H, Tonogi M, Oki H, Maeno M. Angiotensin II suppresses osteoblastic differentiation and mineralized nodule formation via AT1 receptor in ROS17/2.8 cells. Arch Med Sci. 2015 Jun 19;11(3):628-37. doi: 10.5114/aoms.2015.52369. PMID: 26170858; PMCID: PMC4495158.
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Arch Med Sci. 2015 Jun 19;11(3):628-37. doi: 10.5114/aoms.2015.52369.

Angiotensin II suppresses osteoblastic differentiation and mineralized nodule formation via AT1 receptor in ROS17/2.8 cells.

Archives of medical science : AMS

Kumiko Nakai, Takayuki Kawato, Toyoko Morita, Yoji Yamazaki, Hideki Tanaka, Morio Tonogi, Hidero Oki, Masao Maeno

Affiliations

  1. Division of Oral Health Sciences, Nihon University Graduate School of Dentistry, Tokyo, Japan.
  2. Department of Oral Health Sciences, Nihon University School of Dentistry, Tokyo, Japan ; Division of Functional Morphology, Dental Research Center, Nihon University School of Dentistry, Tokyo, Japan.
  3. Department of Oral Health Sciences, Nihon University School of Dentistry, Tokyo, Japan ; The Lion Foundation for Dental Health, Tokyo, Japan.
  4. The Lion Foundation for Dental Health, Tokyo, Japan.
  5. Department of Oral and Maxillofacial Surgery, Nihon University School of Dentistry, Tokyo, Japan.
  6. Department of Oral and Maxillofacial Surgery, Nihon University School of Dentistry, Tokyo, Japan ; Division of Immunology and Pathobiology, Dental Research Center, Nihon University School of Dentistry, Tokyo, Japan.

PMID: 26170858 PMCID: PMC4495158 DOI: 10.5114/aoms.2015.52369

Abstract

INTRODUCTION: Angiotensin II (Ang II) not only regulates systemic blood pressure through a vasoconstrictive effect, but also promotes bone resorption. We recently reported that Ang II (10(-6) M) stimulated the production of matrix metalloproteinases via the AT1 receptor in osteoblastic ROS17/2.8 cells, but suppressed alkaline phosphatase activity. However, the roles of Ang II in osteoblastic differentiation and the function of osteogenesis in osteoblasts are unclear. Therefore, we examined the effect of Ang II on the expression of osteogenesis-related transcription factors and extracellular matrix (ECM) proteins, as well as mineralized nodule formation in ROS17/2.8 cells.

MATERIAL AND METHODS: ROS17/2.8 cells were cultured with 0 (control) or 10(-6) M Ang II in the presence or absence of the AT1 receptor blocker losartan. Mineralized nodule formation was detected by Alizarin Red staining. Gene and protein expression levels of transcription factors and ECM proteins were determined using real-time PCR and Western blotting, respectively.

RESULTS: Runx2, Msx2, and osteocalcin expression significantly decreased with Ang II compared to the control, whereas AJ18 expression significantly increased. Osterix, Dlx5, type I collagen, bone sialoprotein, and osteopontin expression was unaffected. Mineralized nodule formation and calcium content in mineralized nodules decreased with Ang II. Losartan blocked suppressive or stimulatory effects of Ang II on Runx2, Msx2, osteocalcin, and AJ18 expression.

CONCLUSIONS: These results suggest that Ang II suppresses osteoblastic differentiation by altering the expression of osteogenesis-related transcription factors via the AT1 receptor and the function of osteogenesis in ROS17/2.8 cells.

Keywords: angiotensin; extracellular matrix proteins; osteoblast; transcription factors

References

  1. Lab Invest. 1989 Mar;60(3):320-30 - PubMed
  2. J Cell Biochem. 2006 Dec 1;99(5):1233-9 - PubMed
  3. Arch Oral Biol. 2011 Mar;56(3):205-11 - PubMed
  4. Arthritis Rheum. 2012 Oct;64(10 ):3355-63 - PubMed
  5. Med Oral Patol Oral Cir Bucal. 2013 Jan 01;18(1):e93-9 - PubMed
  6. Development. 1999 Sep;126(17):3795-809 - PubMed
  7. Nature. 1970 Aug 15;227(5259):680-5 - PubMed
  8. J Endocrinol. 1997 Jan;152(1):5-10 - PubMed
  9. J Cell Physiol. 1998 Apr;175(1):89-98 - PubMed
  10. Dev Biol. 1999 Jan 15;205(2):260-74 - PubMed
  11. Arch Oral Biol. 2008 Mar;53(3):214-9 - PubMed
  12. J Cell Physiol. 2007 May;211(2):392-8 - PubMed
  13. Int J Periodontics Restorative Dent. 2012 Jun;32(3):285-93 - PubMed
  14. Biochimie. 2013 Apr;95(4):922-33 - PubMed
  15. Mol Cell Biol. 2004 Oct;24(20):9248-61 - PubMed
  16. Crit Rev Oral Biol Med. 1999;10(1):79-98 - PubMed
  17. Development. 2003 Dec;130(24):6131-42 - PubMed
  18. J Oral Sci. 2012 Sep;54(3):251-9 - PubMed
  19. J Endocrinol. 1998 Mar;156(3):543-50 - PubMed
  20. N Engl J Med. 1983 Oct 13;309(15):888-91 - PubMed
  21. Cytokine. 2008 Aug;43(2):165-73 - PubMed
  22. FASEB J. 1997 Mar;11(4):227-33 - PubMed
  23. J Hypertens. 2006 Mar;24(3):581-9 - PubMed
  24. Cell. 1997 May 30;89(5):765-71 - PubMed
  25. Dev Biol. 1999 Apr 1;208(1):123-33 - PubMed
  26. J Dent Res. 2008 Oct;87(10):937-42 - PubMed
  27. Nat Rev Drug Discov. 2012 Mar 01;11(3):234-50 - PubMed
  28. EMBO J. 2003 Dec 15;22(24):6471-82 - PubMed
  29. Int J Mol Sci. 2011;12(7):4206-13 - PubMed
  30. Oral Dis. 2002 May;8(3):147-59 - PubMed
  31. J Nephrol. 2000 May-Jun;13(3):169-77 - PubMed
  32. Trends Mol Med. 2002 Jul;8(7):340-5 - PubMed
  33. Nat Genet. 2000 Apr;24(4):391-5 - PubMed
  34. FASEB J. 2008 Jul;22(7):2465-75 - PubMed
  35. J Bone Miner Res. 2011 Dec;26(12):2959-66 - PubMed
  36. Biochem Pharmacol. 2011 Feb 15;81(4):518-25 - PubMed
  37. Clin Orthop Relat Res. 1992 Aug;(281):275-94 - PubMed
  38. Semin Cell Dev Biol. 2000 Oct;11(5):343-6 - PubMed
  39. J Biol Chem. 2001 May 25;276(21):18282-9 - PubMed
  40. J Biol Chem. 2004 Aug 6;279(32):34015-22 - PubMed
  41. Bone. 2006 Apr;38(4):584-8 - PubMed
  42. J Bone Miner Res. 1993 Dec;8 Suppl 2:S483-7 - PubMed
  43. Biochem Biophys Res Commun. 2007 Oct 19;362(2):368-73 - PubMed
  44. Cell. 2002 Jan 11;108(1):17-29 - PubMed

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