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Colin J, Garibal J, Clavier A, et al. Screening of suppressors of bax-induced cell death identifies glycerophosphate oxidase-1 as a mediator of debcl-induced apoptosis in Drosophila. Genes Cancer. 2015;6(5):241-253doi: 10.18632/genesandcancer.68.
Colin, J., Garibal, J., Clavier, A., Szuplewski, S., Risler, Y., Milet, C., Gaumer, S., Guénal, I., & Mignotte, B. (2015). Screening of suppressors of bax-induced cell death identifies glycerophosphate oxidase-1 as a mediator of debcl-induced apoptosis in Drosophila. Genes & cancer, 6(5), 241-253. https://doi.org/10.18632/genesandcancer.68
Colin, Jessie, et al. "Screening of suppressors of bax-induced cell death identifies glycerophosphate oxidase-1 as a mediator of debcl-induced apoptosis in Drosophila." Genes & cancer vol. 6,5 (2015): 241-253. doi: https://doi.org/10.18632/genesandcancer.68
Colin J, Garibal J, Clavier A, Szuplewski S, Risler Y, Milet C, Gaumer S, Guénal I, Mignotte B. Screening of suppressors of bax-induced cell death identifies glycerophosphate oxidase-1 as a mediator of debcl-induced apoptosis in Drosophila. Genes Cancer. 2015 May;6(5):241-253. doi: 10.18632/genesandcancer.68. PMID: 26124923; PMCID: PMC4482245.
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Genes Cancer. 2015 May;6(5):241-253. doi: 10.18632/genesandcancer.68.

Screening of suppressors of bax-induced cell death identifies glycerophosphate oxidase-1 as a mediator of debcl-induced apoptosis in Drosophila.

Genes & cancer

Jessie Colin, Julie Garibal, Amandine Clavier, Sébastien Szuplewski, Yanick Risler, Cécile Milet, Sébastien Gaumer, Isabelle Guénal, Bernard Mignotte

Affiliations

  1. Université Versailles St-Quentin, Laboratoire de Génétique et Biologie Cellulaire, Montigny-le-Bretonneux, France.
  2. Ecole Pratique des Hautes Etudes, Laboratoire de Génétique Moléculaire et Physiologique, Montigny-le-Bretonneux, France.
  3. Co-senior authors.

PMID: 26124923 PMCID: PMC4482245 DOI: 10.18632/genesandcancer.68

Abstract

Members of the Bcl-2 family are key elements of the apoptotic machinery. In mammals, this multigenic family contains about twenty members, which either promote or inhibit apoptosis. We have previously shown that the mammalian pro-apoptotic Bcl-2 family member Bax is very efficient in inducing apoptosis in Drosophila, allowing the study of bax-induced cell death in a genetic animal model. We report here the results of the screening of a P[UAS]-element insertion library performed to identify gene products that modify the phenotypes induced by the expression of bax in Drosophila melanogaster. We isolated 17 putative modifiers involved in various function or process: the ubiquitin/proteasome pathway; cell growth, proliferation and death; pathfinding and cell adhesion; secretion and extracellular signaling; metabolism and oxidative stress. Most of these suppressors also inhibit debcl-induced phenotypes, suggesting that the activities of both proteins can be modulated in part by common signaling or metabolic pathways. Among these suppressors, Glycerophosphate oxidase-1 is found to participate in debcl-induced apoptosis by increasing mitochondrial reactive oxygen species accumulation.

Keywords: Apoptosis; Bax; Debcl; Glycerophosphate oxidase; mutagenesis

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