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Penrod RD, Campagna J, Panneck T, et al. The presence of cortical neurons in striatal-cortical co-cultures alters the effects of dopamine and BDNF on medium spiny neuron dendritic development. Front Cell Neurosci. 2015;9:269doi: 10.3389/fncel.2015.00269.
Penrod, R. D., Campagna, J., Panneck, T., Preese, L., & Lanier, L. M. (2015). The presence of cortical neurons in striatal-cortical co-cultures alters the effects of dopamine and BDNF on medium spiny neuron dendritic development. Frontiers in cellular neuroscience, 9269. https://doi.org/10.3389/fncel.2015.00269
Penrod, Rachel D, et al. "The presence of cortical neurons in striatal-cortical co-cultures alters the effects of dopamine and BDNF on medium spiny neuron dendritic development." Frontiers in cellular neuroscience vol. 9 (2015): 269. doi: https://doi.org/10.3389/fncel.2015.00269
Penrod RD, Campagna J, Panneck T, Preese L, Lanier LM. The presence of cortical neurons in striatal-cortical co-cultures alters the effects of dopamine and BDNF on medium spiny neuron dendritic development. Front Cell Neurosci. 2015 Jul 20;9:269. doi: 10.3389/fncel.2015.00269. eCollection 2015. PMID: 26257605; PMCID: PMC4507052.
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Front Cell Neurosci. 2015 Jul 20;9:269. doi: 10.3389/fncel.2015.00269. eCollection 2015.

The presence of cortical neurons in striatal-cortical co-cultures alters the effects of dopamine and BDNF on medium spiny neuron dendritic development.

Frontiers in cellular neuroscience

Rachel D Penrod, Justin Campagna, Travis Panneck, Laura Preese, Lorene M Lanier

Affiliations

  1. Department of Neuroscience, University of Minnesota Minneapolis, MN, USA ; Graduate Program in Neuroscience, University of Minnesota Minneapolis, MN, USA.
  2. Department of Neuroscience, University of Minnesota Minneapolis, MN, USA.

PMID: 26257605 PMCID: PMC4507052 DOI: 10.3389/fncel.2015.00269

Abstract

Medium spiny neurons (MSNs) are the major striatal neuron and receive synaptic input from both glutamatergic and dopaminergic afferents. These synapses are made on MSN dendritic spines, which undergo density and morphology changes in association with numerous disease and experience-dependent states. Despite wide interest in the structure and function of mature MSNs, relatively little is known about MSN development. Furthermore, most in vitro studies of MSN development have been done in simple striatal cultures that lack any type of non-autologous synaptic input, leaving open the question of how MSN development is affected by a complex environment that includes other types of neurons, glia, and accompanying secreted and cell-associated cues. Here we characterize the development of MSNs in striatal-cortical co-culture, including quantitative morphological analysis of dendritic arborization and spine development, describing progressive changes in density and morphology of developing spines. Overall, MSN growth is much more robust in the striatal-cortical co-culture compared to striatal mono-culture. Inclusion of dopamine (DA) in the co-culture further enhances MSN dendritic arborization and spine density, but the effects of DA on dendritic branching are only significant at later times in development. In contrast, exogenous Brain Derived Neurotrophic Factor (BDNF) has only a minimal effect on MSN development in the co-culture, but significantly enhances MSN dendritic arborization in striatal mono-culture. Importantly, inhibition of NMDA receptors in the co-culture significantly enhances the effect of exogenous BDNF, suggesting that the efficacy of BDNF depends on the cellular environment. Combined, these studies identify specific periods of MSN development that may be particularly sensitive to perturbation by external factors and demonstrate the importance of studying MSN development in a complex signaling environment.

Keywords: BDNF; dendritic branching; dendritic spines; development; dopamine; in vitro; medium spiny neurons (MSN); striatum

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