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Burton JC, Wang S, Stewart CA, et al. High-resolution three-dimensional in vivo imaging of mouse oviduct using optical coherence tomography. Biomed Opt Express. 2015;6(7):2713-23doi: 10.1364/BOE.6.002713.
Burton, J. C., Wang, S., Stewart, C. A., Behringer, R. R., & Larina, I. V. (2015). High-resolution three-dimensional in vivo imaging of mouse oviduct using optical coherence tomography. Biomedical optics express, 6(7), 2713-23. https://doi.org/10.1364/BOE.6.002713
Burton, Jason C, et al. "High-resolution three-dimensional in vivo imaging of mouse oviduct using optical coherence tomography." Biomedical optics express vol. 6,7 (2015): 2713-23. doi: https://doi.org/10.1364/BOE.6.002713
Burton JC, Wang S, Stewart CA, Behringer RR, Larina IV. High-resolution three-dimensional in vivo imaging of mouse oviduct using optical coherence tomography. Biomed Opt Express. 2015 Jun 30;6(7):2713-23. doi: 10.1364/BOE.6.002713. eCollection 2015 Jul 01. PMID: 26203393; PMCID: PMC4505721.
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Biomed Opt Express. 2015 Jun 30;6(7):2713-23. doi: 10.1364/BOE.6.002713. eCollection 2015 Jul 01.

High-resolution three-dimensional in vivo imaging of mouse oviduct using optical coherence tomography.

Biomedical optics express

Jason C Burton, Shang Wang, C Allison Stewart, Richard R Behringer, Irina V Larina

Affiliations

  1. Department of Molecular Physiology and Biophysics, Baylor College of Medicine, One Baylor Plaza, Houston, Texas, 77030, USA ; Equal Contribution.
  2. Department of Genetics, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Blvd., Houston, Texas, 77030, USA.
  3. Department of Molecular Physiology and Biophysics, Baylor College of Medicine, One Baylor Plaza, Houston, Texas, 77030, USA.

PMID: 26203393 PMCID: PMC4505721 DOI: 10.1364/BOE.6.002713

Abstract

The understanding of the reproductive events and the molecular mechanisms regulating fertility and infertility in humans relies heavily on the analysis of the corresponding phenotypes in mouse models. While molecular genetic approaches provide significant insight into the molecular regulation of these processes, the lack of live imaging methods that allow for detailed visualization of the mouse reproductive organs limits our investigations of dynamic events taking place during the ovulation, the fertilization and the pre-implantation stages of embryonic development. Here we introduce an in vivo three-dimensional imaging approach for visualizing the mouse oviduct and reproductive events with micro-scale spatial resolution using optical coherence tomography (OCT). This method relies on the natural tissue optical contrast and does not require the application of any contrast agents. For the first time, we present live high-resolution images of the internal structural features of the oviduct, as well as other reproductive organs and the oocytes surrounded by cumulus cells. These results provide the basis for a wide range of live dynamic studies focused on understanding fertility and infertility.

Keywords: (110.4500) Optical coherence tomography; (170.3880) Medical and biological imaging; (170.5380) Physiology

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