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Sharma D, Philip G, Gabrani R, et al. Dual agents loaded polymeric nanoparticle: Effect of process variables. Int J Pharm Investig. 2015;5(3):155-60doi: 10.4103/2230-973X.160853.
Sharma, D., Philip, G., Gabrani, R., Ali, J., & Dang, S. (2015). Dual agents loaded polymeric nanoparticle: Effect of process variables. International journal of pharmaceutical investigation, 5(3), 155-60. https://doi.org/10.4103/2230-973X.160853
Sharma, Deepak, et al. "Dual agents loaded polymeric nanoparticle: Effect of process variables." International journal of pharmaceutical investigation vol. 5,3 (2015): 155-60. doi: https://doi.org/10.4103/2230-973X.160853
Sharma D, Philip G, Gabrani R, Ali J, Dang S. Dual agents loaded polymeric nanoparticle: Effect of process variables. Int J Pharm Investig. 2015 Jul-Sep;5(3):155-60. doi: 10.4103/2230-973X.160853. PMID: 26258057; PMCID: PMC4522865.
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Int J Pharm Investig. 2015 Jul-Sep;5(3):155-60. doi: 10.4103/2230-973X.160853.

Dual agents loaded polymeric nanoparticle: Effect of process variables.

International journal of pharmaceutical investigation

Deepak Sharma, Gilphy Philip, Reema Gabrani, Javed Ali, Shweta Dang

Affiliations

  1. Department of Biotechnology, Jaypee Institute of Information Technology, Noida, Uttar Pradesh, India.
  2. Faculty of Pharmacy, Jamia Hamdard, Hamdard Nagar, New Delhi, India.

PMID: 26258057 PMCID: PMC4522865 DOI: 10.4103/2230-973X.160853

Abstract

AIM AND OBJECTIVES: In the present investigation dual agents i.e., hesperidin and diazepam loaded polymeric nanoparticles (NPs) were formulated by nanoprecipitation method and optimized using three-level factorial design.

METHODS: The developed NPs were optimized keeping poly (lactic-co-glycolic) acid (PLGA), poloxamer amount as independent process variable and z-average, percentage drug entrapment as a dependent response. The optimized NP was subjected to in vitro drug release study to investigate drug release mechanism from NP. Cell viability assay was performed on Vero cell line to confirm the safety of NP.

RESULTS: Drug loaded NP showed z-average in the range of 189-307 d.nm with percentage drug entrapment for diazepam and hesperidin 62-89% and 68-92%, respectively. In vitro drug release studies showed controlled drug release behavior was observed from polymeric NP across dialysis membrane compared to aqueous drug solution. Cell viability assay showed drug dependent cytotoxicity on Vero cell line, however, polymeric NP showed less cytotoxicity compared with aqueous drug solution.

Keywords: Polymeric nanoparticles; Three levels factorial design; diazepam; hesperidin; optimization

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