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Dutta S, Reamtong O, Panvongsa W, et al. Proteomics profiling of cholangiocarcinoma exosomes: A potential role of oncogenic protein transferring in cancer progression. Biochim Biophys Acta. 2015;1852(9):1989-99doi: 10.1016/j.bbadis.2015.06.024.
Dutta, S., Reamtong, O., Panvongsa, W., Kitdumrongthum, S., Janpipatkul, K., Sangvanich, P., Piyachaturawat, P., & Chairoungdua, A. (2015). Proteomics profiling of cholangiocarcinoma exosomes: A potential role of oncogenic protein transferring in cancer progression. Biochimica et biophysica acta, 1852(9), 1989-99. https://doi.org/10.1016/j.bbadis.2015.06.024
Dutta, Suman, et al. "Proteomics profiling of cholangiocarcinoma exosomes: A potential role of oncogenic protein transferring in cancer progression." Biochimica et biophysica acta vol. 1852,9 (2015): 1989-99. doi: https://doi.org/10.1016/j.bbadis.2015.06.024
Dutta S, Reamtong O, Panvongsa W, Kitdumrongthum S, Janpipatkul K, Sangvanich P, Piyachaturawat P, Chairoungdua A. Proteomics profiling of cholangiocarcinoma exosomes: A potential role of oncogenic protein transferring in cancer progression. Biochim Biophys Acta. 2015 Sep;1852(9):1989-99. doi: 10.1016/j.bbadis.2015.06.024. Epub 2015 Jul 03. PMID: 26148937.
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Biochim Biophys Acta. 2015 Sep;1852(9):1989-99. doi: 10.1016/j.bbadis.2015.06.024. Epub 2015 Jul 03.

Proteomics profiling of cholangiocarcinoma exosomes: A potential role of oncogenic protein transferring in cancer progression.

Biochimica et biophysica acta

Suman Dutta, Onrapak Reamtong, Wittaya Panvongsa, Sarunya Kitdumrongthum, Keatdamrong Janpipatkul, Polkit Sangvanich, Pawinee Piyachaturawat, Arthit Chairoungdua

Affiliations

  1. Department of Physiology, Faculty of Science, Mahidol University, Bangkok, Thailand; Research Center of Transport Proteins for Medical Innovation, Faculty of Science, Mahidol University, Bangkok, Thailand.
  2. Department of Molecular Tropical Medicine and Genetics, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
  3. Research Center of Transport Proteins for Medical Innovation, Faculty of Science, Mahidol University, Bangkok, Thailand; Toxicology Graduate Program, Faculty of Science, Mahidol University, Bangkok, Thailand.
  4. Department of Physiology, Faculty of Science, Mahidol University, Bangkok, Thailand; Research Center of Transport Proteins for Medical Innovation, Faculty of Science, Mahidol University, Bangkok, Thailand; Department of Basic Medical Science, Faculty of Medicine Vajira Hospital, Navamindradhiraj University, Bangkok, Thailand.
  5. Department of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok, Thailand.
  6. Department of Physiology, Faculty of Science, Mahidol University, Bangkok, Thailand; Research Center of Transport Proteins for Medical Innovation, Faculty of Science, Mahidol University, Bangkok, Thailand; Toxicology Graduate Program, Faculty of Science, Mahidol University, Bangkok, Thailand; Chakri Naruebodindra Medical Institute, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand.
  7. Department of Physiology, Faculty of Science, Mahidol University, Bangkok, Thailand; Research Center of Transport Proteins for Medical Innovation, Faculty of Science, Mahidol University, Bangkok, Thailand; Toxicology Graduate Program, Faculty of Science, Mahidol University, Bangkok, Thailand. Electronic address: [email protected].

PMID: 26148937 DOI: 10.1016/j.bbadis.2015.06.024

Abstract

Cholangiocarcinoma (CCA), a common primary malignant tumor of bile duct epithelia, is highly prevalent in Asian countries and unresponsive to chemotherapeutic drugs. Thus, a newly recognized biological entity for early diagnosis and treatment is highly needed. Exosomes are small membrane bound vesicles found in body fluids and released by most cell types including cancer cells. The vesicles contain specific subset of proteins and nucleic acids corresponding to cell types and play essential roles in pathophysiological processes. The present study aimed to assess the protein profiles of CCA-derived exosomes and their potential roles. We have isolated exosomes from CCA cells namely KKU-M213 and KKU-100 derived from Thai patients and their roles were investigated by incubation with normal human cholangiocyte (H69) cells. Exosomes were internalized into H69 cells and had no effects on viability or proliferation of the host cells. Interestingly, the exosomes from KKU-M213 cells only induced migration and invasion of H69 cells. Proteomic analysis of the exosomes from KKU-M213 cells disclosed multiple cancer related proteins that are not present in H69 exosomes. Consistent with the protein profile, treatment with KKU-M213 exosomes induced β-catenin and reduced E-cadherin expressions in H69 cells. Collectively, our results suggest that a direct cell-to-cell transfer of oncogenic proteins via exosomal pathway may be a novel mechanism for CCA progression and metastasis.

Copyright © 2015 Elsevier B.V. All rights reserved.

Keywords: Cell–cell communication; Cholangiocarcinoma; Exosomes; Invasion; Migration; Proteomics

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