Display options
Cite
Lee YS, Lee DY, Kim YB, et al. The Mechanism Underlying the Antibacterial Activity of Shikonin against Methicillin-Resistant Staphylococcus aureus. Evid Based Complement Alternat Med. 2015;2015:520578doi: 10.1155/2015/520578.
Lee, Y. S., Lee, D. Y., Kim, Y. B., Lee, S. W., Cha, S. W., Park, H. W., Kim, G. S., Kwon, D. Y., Lee, M. H., & Han, S. H. (2015). The Mechanism Underlying the Antibacterial Activity of Shikonin against Methicillin-Resistant Staphylococcus aureus. Evidence-based complementary and alternative medicine : eCAM, 2015520578. https://doi.org/10.1155/2015/520578
Lee, Young-Seob, et al. "The Mechanism Underlying the Antibacterial Activity of Shikonin against Methicillin-Resistant Staphylococcus aureus." Evidence-based complementary and alternative medicine : eCAM vol. 2015 (2015): 520578. doi: https://doi.org/10.1155/2015/520578
Lee YS, Lee DY, Kim YB, Lee SW, Cha SW, Park HW, Kim GS, Kwon DY, Lee MH, Han SH. The Mechanism Underlying the Antibacterial Activity of Shikonin against Methicillin-Resistant Staphylococcus aureus. Evid Based Complement Alternat Med. 2015;2015:520578. doi: 10.1155/2015/520578. Epub 2015 Jul 21. PMID: 26265924; PMCID: PMC4523682.
Copy Download .nbib Format: NLM
Share it on

Evid Based Complement Alternat Med. 2015;2015:520578. doi: 10.1155/2015/520578. Epub 2015 Jul 21.

The Mechanism Underlying the Antibacterial Activity of Shikonin against Methicillin-Resistant Staphylococcus aureus.

Evidence-based complementary and alternative medicine : eCAM

Young-Seob Lee, Dae-Young Lee, Yeon Bok Kim, Sang-Won Lee, Seon-Woo Cha, Hong-Woo Park, Geum-Soog Kim, Dong-Yeul Kwon, Min-Ho Lee, Sin-Hee Han

Affiliations

  1. Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration (RDA), Eumsung, Chungbuk 369-873, Republic of Korea.
  2. Department of Oriental Pharmacy, College of Pharmacy and Wonkwang-Oriental Medicines Research Institute, Wonkwang University, Iksan, Jeonbuk 570-749, Republic of Korea.
  3. Department of Food Technology and Services, Eulji University, Seongnam, Gyeonggi 461-713, Republic of Korea.

PMID: 26265924 PMCID: PMC4523682 DOI: 10.1155/2015/520578

Abstract

Shikonin (SKN), a highly liposoluble naphthoquinone pigment isolated from the roots of Lithospermum erythrorhizon, is known to exert antibacterial, wound-healing, anti-inflammatory, antithrombotic, and antitumor effects. The aim of this study was to examine SKN antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA). The SKN was analyzed in combination with membrane-permeabilizing agents Tris and Triton X-100, ATPase inhibitors sodium azide and N,N'-dicyclohexylcarbodiimide, and S. aureus-derived peptidoglycan; the effects on MRSA viability were evaluated by the broth microdilution method, time-kill test, and transmission electron microscopy. Addition of membrane-permeabilizing agents or ATPase inhibitors together with a low dose of SKN potentiated SKN anti-MRSA activity, as evidenced by the reduction of MRSA cell density by 75% compared to that observed when SKN was used alone; in contrast, addition of peptidoglycan blocked the antibacterial activity of SKN. The results indicate that the anti-MRSA effect of SKN is associated with its affinity to peptidoglycan, the permeability of the cytoplasmic membrane, and the activity of ATP-binding cassette (ABC) transporters. This study revealed the potential of SKN as an effective natural antibiotic and of its possible use to substantially reduce the use of existing antibiotic may also be important for understanding the mechanism underlying the antibacterial activity of natural compounds.

References

  1. J Infect Dis. 1978 Dec;138(6):865-71 - PubMed
  2. J Agric Food Chem. 1999 Oct;47(10):4117-20 - PubMed
  3. J Antimicrob Chemother. 2000 Apr;45(4):421-31 - PubMed
  4. Antimicrob Agents Chemother. 2001 Jun;45(6):1737-42 - PubMed
  5. Nat Struct Biol. 2002 Nov;9(11):870-6 - PubMed
  6. J Nat Prod. 2002 Dec;65(12):1857-62 - PubMed
  7. J Clin Invest. 2003 May;111(9):1265-73 - PubMed
  8. Clin Diagn Lab Immunol. 2003 Sep;10(5):950-3 - PubMed
  9. J Biol Chem. 2004 Feb 13;279(7):5877-85 - PubMed
  10. Antimicrob Agents Chemother. 2004 Apr;48(4):1361-4 - PubMed
  11. Biochemistry. 2004 Jun 15;43(23):7491-502 - PubMed
  12. Clin Infect Dis. 2004 Sep 15;39(6):776-82 - PubMed
  13. Am J Med. 2006 Jun;119(6 Suppl 1):S3-10; discussion S62-70 - PubMed
  14. Cell. 2007 Mar 23;128(6):1037-50 - PubMed
  15. BMC Infect Dis. 2007 Sep 21;7:111 - PubMed
  16. J Microbiol Biotechnol. 2007 Aug;17(8):1324-9 - PubMed
  17. J Microbiol. 2008 Aug;46(4):462-7 - PubMed
  18. Curr Mol Med. 2009 Mar;9(2):100-15 - PubMed
  19. Clin Infect Dis. 2009 Jul 1;49(1):65-71 - PubMed
  20. Foodborne Pathog Dis. 2010 Apr;7(4):435-41 - PubMed
  21. J Toxicol Sci. 2010 Jun;35(3):357-64 - PubMed
  22. Antimicrob Agents Chemother. 2011 Feb;55(2):910-2 - PubMed
  23. Expert Rev Anti Infect Ther. 2011 Aug;9(8):653-79 - PubMed
  24. Eur Rev Med Pharmacol Sci. 2012 Jul;16(7):853-9 - PubMed
  25. Exp Ther Med. 2012 Apr;3(4):608-612 - PubMed
  26. Molecules. 2012 Dec 03;17(12):14310-22 - PubMed
  27. Evid Based Complement Alternat Med. 2013;2013:379536 - PubMed
  28. Structure. 2013 Oct 8;21(10):1879-88 - PubMed
  29. Foodborne Pathog Dis. 2014 Mar;11(3):234-9 - PubMed
  30. Evid Based Complement Alternat Med. 2014;2014:716509 - PubMed
  31. Angew Chem Int Ed Engl. 1999 Feb 1;38(3):270-301 - PubMed
  32. J Hosp Infect. 1995 Feb;29(2):87-106 - PubMed

Publication Types