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Szymańska-Kałuża J, Cebula-Obrzut B, Smolewski P, et al. Imbalance of Th17 and T-regulatory cells in peripheral blood and synovial fluid in treatment naïve children with juvenile idiopathic arthritis. Cent Eur J Immunol. 2014;39(1):71-6doi: 10.5114/ceji.2014.42128.
Szymańska-Kałuża, J., Cebula-Obrzut, B., Smolewski, P., Stanczyk, J., & Smolewska, E. (2014). Imbalance of Th17 and T-regulatory cells in peripheral blood and synovial fluid in treatment naïve children with juvenile idiopathic arthritis. Central-European journal of immunology, 39(1), 71-6. https://doi.org/10.5114/ceji.2014.42128
Szymańska-Kałuża, Joanna, et al. "Imbalance of Th17 and T-regulatory cells in peripheral blood and synovial fluid in treatment naïve children with juvenile idiopathic arthritis." Central-European journal of immunology vol. 39,1 (2014): 71-6. doi: https://doi.org/10.5114/ceji.2014.42128
Szymańska-Kałuża J, Cebula-Obrzut B, Smolewski P, Stanczyk J, Smolewska E. Imbalance of Th17 and T-regulatory cells in peripheral blood and synovial fluid in treatment naïve children with juvenile idiopathic arthritis. Cent Eur J Immunol. 2014;39(1):71-6. doi: 10.5114/ceji.2014.42128. Epub 2014 Apr 17. PMID: 26155103; PMCID: PMC4439971.
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Cent Eur J Immunol. 2014;39(1):71-6. doi: 10.5114/ceji.2014.42128. Epub 2014 Apr 17.

Imbalance of Th17 and T-regulatory cells in peripheral blood and synovial fluid in treatment naïve children with juvenile idiopathic arthritis.

Central-European journal of immunology

Joanna Szymańska-Kałuża, Barbara Cebula-Obrzut, Piotr Smolewski, Jerzy Stanczyk, Elżbieta Smolewska

Affiliations

  1. Department of Pediatric Cardiology and Rheumatology, Medical University of Lodz, Poland.
  2. Department of Experimental Hematology, Medical University of Lodz, Poland.

PMID: 26155103 PMCID: PMC4439971 DOI: 10.5114/ceji.2014.42128

Abstract

OBJECTIVES: The imbalance between Th17 and T regulatory cells (Tregs) may be a key event in development of autoimmunity. The problem is poorly explored in juvenile idiopathic arthritis (JIA) so far. In this study, peripheral blood (PB) and synovial fluid (SF) Tregs and Th17 cells from were assessed in untreated JIA children.

MATERIAL AND METHODS: In 50 children with JIA the PB or SF percentages of Tregs and Th17 cells were assessed by flow cytometry, in comparison with PB Tregs and Th17 cells from 28 healthy controls. Additionally, in both groups the levels of proinfammatory cytokines, such as interleukin (IL)-1β, IL -6, IL -17, IL -21, IL -23 and tumor necrosis factor α (TN F-α) were assessed using ELI SA method.

RESULTS: The proportion of JIA PB Th17 cells was significantly higher than in the controls (p = 0.01). Serum levels of IL -1β, IL -6, IL -17, IL -23 were also significantly higher in JIA (p = 0.011, p = 0.007, p = 0.008 and p = 0.023, respectively). The highest serum IL -6 levels were observed in oligoarthritis JIA (p = 0.031). Synovial fluid IL -21 concentration was distinctly higher in polyarticular JIA. Synovial fluid levels of TN F-α, IL -1β and IL -6 were significantly higher than in JIA PB (p = 0.038, p = 0.013 and p < 0.001, respectively). There was a significant correlation between IL -6 and PB Tregs (p = 0.02).

CONCLUSIONS: The results of this comprehensive analysis indicate a role of Th17 cell activation in the pathogenesis of JIA.

Keywords: T-regulatory cells; Th17 cells; juvenile idiopathic arthritis; proinflammatory cytokines

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