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Milanova V, Ivanovska N, Dimitrova P. The effect of interleukin 17 and Toll-like receptor 2 on CD11b expression and apoptosis of neutrophils in zymosaninduced arthritis and paw oedema. Cent Eur J Immunol. 2014;39(2):131-41doi: 10.5114/ceji.2014.43712.
Milanova, V., Ivanovska, N., & Dimitrova, P. (2014). The effect of interleukin 17 and Toll-like receptor 2 on CD11b expression and apoptosis of neutrophils in zymosaninduced arthritis and paw oedema. Central-European journal of immunology, 39(2), 131-41. https://doi.org/10.5114/ceji.2014.43712
Milanova, Viktoriya, et al. "The effect of interleukin 17 and Toll-like receptor 2 on CD11b expression and apoptosis of neutrophils in zymosaninduced arthritis and paw oedema." Central-European journal of immunology vol. 39,2 (2014): 131-41. doi: https://doi.org/10.5114/ceji.2014.43712
Milanova V, Ivanovska N, Dimitrova P. The effect of interleukin 17 and Toll-like receptor 2 on CD11b expression and apoptosis of neutrophils in zymosaninduced arthritis and paw oedema. Cent Eur J Immunol. 2014;39(2):131-41. doi: 10.5114/ceji.2014.43712. Epub 2014 Jun 27. PMID: 26155114; PMCID: PMC4440028.
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Cent Eur J Immunol. 2014;39(2):131-41. doi: 10.5114/ceji.2014.43712. Epub 2014 Jun 27.

The effect of interleukin 17 and Toll-like receptor 2 on CD11b expression and apoptosis of neutrophils in zymosaninduced arthritis and paw oedema.

Central-European journal of immunology

Viktoriya Milanova, Nina Ivanovska, Petya Dimitrova

Affiliations

  1. Department of Immunology, Institute of Microbiology, Sofia, Bulgaria.

PMID: 26155114 PMCID: PMC4440028 DOI: 10.5114/ceji.2014.43712

Abstract

We investigated the effect of interleukin (IL)-17 on Ly6G(+) cell apoptosis in zymosan-induced arthritis (ZIA) and oedema (ZIO). Zymosan injection at the ankle joint caused swelling and coincided with histological joint alterations and IL-17A expression in areas with cell infiltrates. Flow cytometry of blood demonstrated increased frequencies of Ly6G(+)CD11b(+) cells and their decreased apoptosis in ZIA. Annexin V(+) neutrophils had lower CD11b expression, unlike Annexin V(-) cells. Cell survival for 12 hours was affected neither by IL-17 nor by zymosan alone, while both stimuli diminished Annexin V(+) cell frequencies and up-regulated CD11b on Annexin V(-) cells. Interleukin 17 antagonised to the effects of zymosan in 24-hour cultures. The administration of IL-17 in ZIO increased paw thickness, enlarged the blood Ly6G(+) pool, elevated CD11b expression and decreased apoptosis. We suggest that altered neutrophil apoptosis in arthritis can be overcome by anti-IL-17 therapy combined with an inhibition of Toll-like receptor (TLR) 2 and CD11b signalling.

Keywords: CD11b; TLR2 ligand; interleukin 17; neutrophils; paw oedema

References

  1. Nat Rev Rheumatol. 2009 Oct;5(10):549-53 - PubMed
  2. J Leukoc Biol. 2004 Nov;76(5):1047-56 - PubMed
  3. Mediators Inflamm. 2014;2014:643406 - PubMed
  4. J Leukoc Biol. 2001 Nov;70(5):783-92 - PubMed
  5. Blood. 2010 Jan 14;115(2):335-43 - PubMed
  6. Arthritis Rheum. 2010 Feb;62(2):383-91 - PubMed
  7. PLoS One. 2011;6(8):e24048 - PubMed
  8. J Invest Dermatol. 2014 May;134(5):1276-84 - PubMed
  9. Arthritis Res. 2001;3(3):168-77 - PubMed
  10. Nat Immunol. 2014 Feb;15(2):143-51 - PubMed
  11. Respiration. 2013;86(5):367-75 - PubMed
  12. Mol Immunol. 2008 Jan;45(1):160-8 - PubMed
  13. J Leukoc Biol. 2004 Mar;75(3):467-77 - PubMed
  14. PLoS One. 2013 May 06;8(5):e62231 - PubMed
  15. Arthritis Res Ther. 2006;8(4):R120 - PubMed
  16. J Immunol. 2000 Apr 15;164(8):4286-91 - PubMed
  17. Autoimmun Rev. 2010 Jun;9(8):531-5 - PubMed
  18. Proc Natl Acad Sci U S A. 2011 Aug 9;108(32):13135-40 - PubMed
  19. J Immunol. 2008 Sep 15;181(6):4279-86 - PubMed
  20. Eur J Immunol. 2003 May;33(5):1429-38 - PubMed
  21. J Cell Biol. 2000 Dec 11;151(6):1305-20 - PubMed
  22. Arthritis Rheum. 2005 Oct;52(10):3239-47 - PubMed
  23. Circ Res. 2008 Aug 15;103(4):352-9 - PubMed
  24. Ann N Y Acad Sci. 2011 Jan;1217:60-76 - PubMed
  25. Annu Rev Immunol. 2007;25:619-47 - PubMed
  26. J Histochem Cytochem. 2008 Feb;56(2):89-95 - PubMed
  27. FEBS Lett. 2001 Jan 5;487(3):318-22 - PubMed
  28. N Engl J Med. 2009 Oct 15;361(16):1570-83 - PubMed
  29. Ann Rheum Dis. 2013 Feb;72(2):265-70 - PubMed
  30. J Leukoc Biol. 2005 Dec;78(6):1408-18 - PubMed

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