Display options
Cite
Krapfenbauer K, Drucker E, Thurnher D. Identification of tumour-related proteins as potential screening markers by proteome analysis-protein profiles of human saliva as a predictive and prognostic tool. EPMA J. 2014;5(1):20doi: 10.1186/1878-5085-5-20.
Krapfenbauer, K., Drucker, E., & Thurnher, D. (2014). Identification of tumour-related proteins as potential screening markers by proteome analysis-protein profiles of human saliva as a predictive and prognostic tool. The EPMA journal, 5(1), 20. https://doi.org/10.1186/1878-5085-5-20
Krapfenbauer, Kurt, et al. "Identification of tumour-related proteins as potential screening markers by proteome analysis-protein profiles of human saliva as a predictive and prognostic tool." The EPMA journal vol. 5,1 (2014): 20. doi: https://doi.org/10.1186/1878-5085-5-20
Krapfenbauer K, Drucker E, Thurnher D. Identification of tumour-related proteins as potential screening markers by proteome analysis-protein profiles of human saliva as a predictive and prognostic tool. EPMA J. 2014 Nov 28;5(1):20. doi: 10.1186/1878-5085-5-20. eCollection 2014. PMID: 26269723; PMCID: PMC4534024.
Copy Download .nbib Format: NLM
Share it on

EPMA J. 2014 Nov 28;5(1):20. doi: 10.1186/1878-5085-5-20. eCollection 2014.

Identification of tumour-related proteins as potential screening markers by proteome analysis-protein profiles of human saliva as a predictive and prognostic tool.

The EPMA journal

Kurt Krapfenbauer, Elisabeth Drucker, Dietmar Thurnher

Affiliations

  1. Department of Otorhinolaryngology, Head and Neck Surgery, Medical University of Vienna, A-1090 Vienna, Austria.
  2. Department of Molecular Biotechnology, University of Applied Science Vienna, Helmut-Qualtinger-Gasse 2, A-1090 Vienna, Austria.

PMID: 26269723 PMCID: PMC4534024 DOI: 10.1186/1878-5085-5-20

Abstract

The analysis of biomarkers in saliva as a clinical application offers an attractive, simple and rapid diagnostic tool for the short- and long-term monitoring of pathological disorders and drug therapy. The collection of saliva, either in the pure or in its fractionated form, is a relatively easy and non-invasive procedure that is not harmful to the patients and has no complications at all. However, the fluid collection must be clearly defined due to variations in saliva composition, flow rate and day-to-day variability. In order to minimise possible variations, saliva from five patients without squamous cell carcinoma (SCC) pathology and five with suspicion of oral squamous carcinoma (OSCC) were collected and matched at different days and analysed by two-dimensional polyacrylamide gel electrophoresis (2DE-PAGE). Approximately 800 spots were identified, corresponding to 151 different gene products. The list of identified proteins includes a large number of structural proteins like keratins, keratin subunits, enzymes and enzyme inhibitors, cytokines, immunoglobulins as well as amylase and other salivary specific glycoproteins. The majority of proteins that are localised in oral epithelia cells were found as unsolved debris in saliva. One of the identified proteins was significantly overexpressed in OSCC and was selected for further validation by Western blot analysis.

Keywords: Disease monitoring; Individual patient profiles; Oral squamous cell carcinoma; Patient self-management; Predictive medicine; Saliva proteomics; Tailored therapy; Targeted prevention

References

  1. Clin Otolaryngol Allied Sci. 1998 Feb;23(1):82-6 - PubMed
  2. EPMA J. 2014 Apr 07;5(1):6 - PubMed
  3. Proteomics. 2004 Jan;4(1):271-8 - PubMed
  4. Cancer Res. 2003 Jul 15;63(14):3866-71 - PubMed
  5. Kaohsiung J Med Sci. 1999 Mar;15(3):152-8 - PubMed
  6. J Clin Oncol. 2003 Nov 1;21(21):3891-3 - PubMed
  7. EPMA J. 2012 Nov 01;3(1):14 - PubMed
  8. Cancer. 2003 Aug 15;98(4):737-44 - PubMed
  9. J Laryngol Otol. 2006 Feb;120(2):85-6 - PubMed
  10. J Oral Maxillofac Surg. 1998 Dec;56(12):1390-3 - PubMed
  11. Cell. 1991 Aug 9;66(3):589-600 - PubMed
  12. Crit Rev Oral Biol Med. 2001;12(1):55-63 - PubMed
  13. J Can Dent Assoc. 2002 Nov;68(10):617-21 - PubMed
  14. Int J Cancer. 1997 Jan 17;70(2):194-200 - PubMed
  15. Eur J Cancer. 2002 Jan;38(1):99-166 - PubMed
  16. J Proteome Res. 2008 Sep;7(9):3818-29 - PubMed
  17. Oral Oncol. 2000 May;36(3):272-6 - PubMed
  18. Tumour Biol. 1993;14(1):55-8 - PubMed
  19. Science. 1991 Jul 5;253(5015):49-53 - PubMed
  20. Ann N Y Acad Sci. 2001 Sep;945:172-8 - PubMed
  21. Dtsch Z Mund Kiefer Gesichtschir. 1990 Jul-Aug;14(4):254-9 - PubMed
  22. Proteomics. 2004 Aug;4(8):2465-75 - PubMed
  23. Nat Rev Cancer. 2002 Mar;2(3):210-9 - PubMed
  24. Cancer Lett. 2008 May 8;263(1):89-98 - PubMed
  25. J Proteome Res. 2005 Sep-Oct;4(5):1867-71 - PubMed
  26. J Oral Maxillofac Surg. 1997 Sep;55(9):964-6 - PubMed
  27. Cancer Cell. 2004 Apr;5(4):311-6 - PubMed
  28. Dis Markers. 2001;17(2):49-57 - PubMed
  29. Nat Genet. 2000 Mar;24(3):227-35 - PubMed
  30. Dtsch Z Mund Kiefer Gesichtschir. 1989 Jul-Aug;13(4):291-5 - PubMed
  31. Science. 1997 Nov 7;278(5340):1054-9 - PubMed
  32. Cancer Res. 2000 Nov 15;60(22):6293-7 - PubMed
  33. Dtsch Zahn Mund Kieferheilkd Zentralbl. 1992;80(6):351-7 - PubMed
  34. J Craniomaxillofac Surg. 1998 Aug;26(4):243-8 - PubMed
  35. EPMA J. 2010 Dec;1(4):627-31 - PubMed
  36. Proteomics. 2003 Oct;3(10):1912-9 - PubMed
  37. Anticancer Res. 1993 Jan-Feb;13(1):237-40 - PubMed
  38. Anal Quant Cytol Histol. 1993 Oct;15(5):335-40 - PubMed
  39. Cancer Metastasis Rev. 1999;18(1):65-73 - PubMed
  40. Int J Oral Maxillofac Surg. 1993 Feb;22(1):35-8 - PubMed
  41. J Mol Diagn. 2001 Nov;3(4):164-70 - PubMed
  42. Cancer. 1999 Mar 1;85(5):1018-25 - PubMed
  43. BMC Cancer. 2012 Feb 27;12:76 - PubMed
  44. Laryngoscope. 2000 Mar;110(3 Pt 1):374-81 - PubMed
  45. J Dent Res. 2004 Mar;83(3):199-203 - PubMed
  46. Environ Health Perspect. 1991 Jun;93:51-6 - PubMed
  47. J Can Dent Assoc. 2002 Mar;68(3):170-4 - PubMed
  48. J Cutan Pathol. 2000 Jan;27(1):24-9 - PubMed
  49. Biochem J. 2003 May 1;371(Pt 3):927-35 - PubMed
  50. Clin Cancer Res. 2006 Oct 15;12(20 Pt 1):5960-71 - PubMed
  51. Genes Chromosomes Cancer. 2000 Jan;27(1):17-25 - PubMed

Publication Types