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Front Plant Sci. 2015 Jul 10;6:471. doi: 10.3389/fpls.2015.00471. eCollection 2015.

Spatially resolved in vivo plant metabolomics by laser ablation-based mass spectrometry imaging (MSI) techniques: LDI-MSI and LAESI.

Frontiers in plant science

Benjamin Bartels, Aleš Svatoš

Affiliations

  1. Research Group Mass Spectrometry/Proteomics, Max Planck Institute for Chemical Ecology Jena, Germany.

PMID: 26217345 PMCID: PMC4498035 DOI: 10.3389/fpls.2015.00471

Abstract

This short review aims to summarize the current developments and applications of mass spectrometry-based methods for in situ profiling and imaging of plants with minimal or no sample pre-treatment or manipulation. Infrared-laser ablation electrospray ionization and UV-laser desorption/ionization methods are reviewed. The underlying mechanisms of the ionization techniques-namely, laser ablation of biological samples and electrospray ionization-as well as variations of the LAESI ion source for specific targets of interest are described.

Keywords: ambient; electrospray; ionization; laser ablation; mass spectrometry

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